Basic Information | |
---|---|
Species | Arabidopsis lyrata |
Cazyme ID | 497439 |
Family | GH85 |
Protein Properties | Length: 643 Molecular Weight: 72362.5 Isoelectric Point: 7.4587 |
Chromosome | Chromosome/Scaffold: 467 Start: 2647 End: 6190 |
Description | Glycosyl hydrolase family 85 |
View CDS |
External Links |
---|
NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
---|---|---|---|
Family | Start | End | Evalue |
GH85 | 204 | 302 | 6.3e-22 |
AEVAGDRKFDVYMGIDVFGRGSFGGGQWTVNAALDLLKRNNVSAAIFAPGWVYETAQPPNFHTAQNKWWSLVEKSWGIVQTYPQVLPFYSDFNQGFGYH | |||
GH85 | 76 | 201 | 1.7e-32 |
MKGGYVDDKWVQGCENDAGFAIWHWYLMDIFVYFSHSLVTLPPPCWTNTAHRHGVKVKMLATKESAQMYAERMAELATALGFDGWLINIENDIDEEQIPN LKEFVSHLKKVLHLSTPGALVICYPK |
Full Sequence |
---|
Protein Sequence Length: 643 Download |
MPKSNDADVA ESEAVPLLDL AKPSLPISFP IKSLQDLKSR SYFVSFHFQF NRSTVPLRRD 60 SDDLPNRPRV LVCHDMKGGY VDDKWVQGCE NDAGFAIWHW YLMDIFVYFS HSLVTLPPPC 120 WTNTAHRHGV KVKMLATKES AQMYAERMAE LATALGFDGW LINIENDIDE EQIPNLKEFV 180 SHLKKVLHLS TPGALVICYP KLSAEVAGDR KFDVYMGIDV FGRGSFGGGQ WTVNAALDLL 240 KRNNVSAAIF APGWVYETAQ PPNFHTAQNK WWSLVEKSWG IVQTYPQVLP FYSDFNQGFG 300 YHVSLEGRQL SDAPWYNISC QSLQNSMKKT TKISSTSLLR KMFLTFLSKH LNNYYEFDSA 360 REGSFNRGGN IAFRGKLKGD AYFTTRLFKP HLQLSSSPIT ISYSVKSDET SKLGILLSFS 420 SPSQKTKSIL VAPQESIRRF NDMFLQCLAT SAQTVSEWTV HETSLVMDGH TLTEISAFCY 480 RPENLTKSVE YVALLGHISI KDHVQYQQKS ETLLPASSWV IKADNVELVP GNSGSKILRA 540 KLEWRQKHLE DSVLPRYNVY AENVNSTDLR PRKVLEKPRS ETVFLGVVHV PAYYVAELVV 600 ESDVKAVRFV VQACAEDGSL GKLDEALNLL VELEGISSKY IS* |
Functional Domains Download unfiltered results here | ||||||||
---|---|---|---|---|---|---|---|---|
Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
COG4724 | COG4724 | 3.0e-13 | 50 | 425 | 469 | + Endo-beta-N-acetylglucosaminidase D [Carbohydrate transport and metabolism] | ||
pfam03644 | Glyco_hydro_85 | 6.0e-72 | 76 | 302 | 301 | + Glycosyl hydrolase family 85. Family of endo-beta-N-acetylglucosaminidases. These enzymes work on a broad spectrum of substrates. | ||
cd06547 | GH85_ENGase | 2.0e-88 | 71 | 324 | 329 | + Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the protein and N-acetylglucosamine 2 forms the reducing end of the released glycan. ENGase is a key enzyme in the processing of free oligosaccharides in the cytosol of eukaryotes. Oligosaccharides formed in the lumen of the endoplasmic reticulum are transported into the cytosol where they are catabolized by cytosolic ENGases and other enzymes, possibly to maximize the reutilization of the component sugars. ENGases have an eight-stranded alpha/beta barrel topology and are classified as a family 85 glycosyl hydrolase (GH85) domain. The GH85 ENGases are sequence-similar to the family 18 glycosyl hydrolases, also known as GH18 chitinases. An ENGase-like protein is also found in bacteria and is included in this alignment model. |
Gene Ontology | |
---|---|
GO Term | Description |
GO:0005737 | cytoplasm |
GO:0033925 | mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity |
Annotations - NR Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
RefSeq | NP_187715.1 | 0 | 1 | 637 | 1 | 697 | glycosyl hydrolase family 85 protein [Arabidopsis thaliana] |
RefSeq | NP_196165.3 | 0 | 21 | 639 | 16 | 673 | hydrolase, acting on glycosyl bonds / mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase [Arabidopsis thaliana] |
RefSeq | XP_002273683.1 | 0 | 10 | 635 | 8 | 689 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002315137.1 | 0 | 24 | 635 | 26 | 698 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002315138.1 | 0 | 16 | 635 | 19 | 695 | predicted protein [Populus trichocarpa] |
Annotations - PDB Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3fhq_F | 0.0000000001 | 99 | 456 | 83 | 500 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
PDB | 3fhq_D | 0.0000000001 | 99 | 456 | 83 | 500 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
PDB | 3fhq_B | 0.0000000001 | 99 | 456 | 83 | 500 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
PDB | 3fhq_A | 0.0000000001 | 99 | 456 | 83 | 500 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
PDB | 3fha_D | 0.0000000001 | 99 | 456 | 83 | 500 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
EST Download unfiltered results here | ||||
---|---|---|---|---|
Hit | Length | Start | End | EValue |
EY940981 | 258 | 224 | 481 | 0 |
FY440931 | 231 | 409 | 636 | 0 |
EV160308 | 245 | 118 | 307 | 0 |
EX907560 | 209 | 431 | 637 | 0 |
BY824593 | 179 | 1 | 159 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
---|