| Basic Information | |
|---|---|
| Species | Arabidopsis lyrata |
| Cazyme ID | 875662 |
| Family | GH32 |
| Protein Properties | Length: 655 Molecular Weight: 73209.5 Isoelectric Point: 6.2898 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 116 | 436 | 0 |
| HFQPEKNWMNDPNGPLFYKGWYHFFYQYNPNAAVWGDIVWGHAVSKDLIHWLYLPIAMVPDQWYDANGVWTGSATFLDDGSIVMLYTGSTDKFVQVQNLA YPDDPSDPLLLKWAKFSGNPVLVPPPGIGAKDFRDPTTAWKTSTGKWRITIGSKINRTGISLIYDTTDFKTYEKHDTLLHQVPNTGMWECVDFYPVSKTQ VNGLDTSVNGPDVKHVIKASMDDTRIDHYAIGTYYDSNGTWVPDNPSIDVGISTALRYDYGKFYASKTFYDQNKGRRILWGWIGESDSEAADVQKGWSSV QGIPRTVVLDTRTRKNLVQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 655 Download |
| MASNEALLPV TSRQDLLSES RSDQIPETRR RRPIKVHLAV YSGLLLIALY VALIVTHDGS 60 TSQHGAKDET STESRARLAG VSEKSNDRVS RLSDDRKVEA FPWNNTMLSW QRTAFHFQPE 120 KNWMNDPNGP LFYKGWYHFF YQYNPNAAVW GDIVWGHAVS KDLIHWLYLP IAMVPDQWYD 180 ANGVWTGSAT FLDDGSIVML YTGSTDKFVQ VQNLAYPDDP SDPLLLKWAK FSGNPVLVPP 240 PGIGAKDFRD PTTAWKTSTG KWRITIGSKI NRTGISLIYD TTDFKTYEKH DTLLHQVPNT 300 GMWECVDFYP VSKTQVNGLD TSVNGPDVKH VIKASMDDTR IDHYAIGTYY DSNGTWVPDN 360 PSIDVGISTA LRYDYGKFYA SKTFYDQNKG RRILWGWIGE SDSEAADVQK GWSSVQGIPR 420 TVVLDTRTRK NLVQWPVEEI KSLRLSSNKF DMTIGPGTVV PVDVGSATQL DIEAEFEIKT 480 EDLELFFDDD SVEADKKFSC ETSGGSTARG ALGPFGFSVL ADESLSEQTP VYFYVTKGKH 540 SKLKTVFCTD TSRSTMANDV VKPIYGSFVP VLKGEKLTMR ILVDHSIVEG FAQGGRTCIT 600 SRVYPTKAIY GATKLFLFNN AIDATVTASF KVWQMNSAFI HPYSPDDLSV PSRT* 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 2.0e-55 | 112 | 605 | 512 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 1.0e-70 | 112 | 634 | 536 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 1.0e-94 | 122 | 439 | 324 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 2.0e-151 | 116 | 436 | 329 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 0 | 116 | 595 | 490 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004564 | beta-fructofuranosidase activity |
| GO:0004575 | sucrose alpha-glucosidase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAD43622.1 | 0 | 1 | 654 | 1 | 650 | AC005698_21 T3P18.21 [Arabidopsis thaliana] |
| GenBank | AAF19535.1 | 0 | 1 | 648 | 1 | 644 | AC007190_3 F23N19.3 [Arabidopsis thaliana] |
| GenBank | AAK62665.1 | 0 | 1 | 654 | 1 | 648 | At1g62660/F23N19_3 [Arabidopsis thaliana] |
| EMBL | CAA64781.1 | 0 | 10 | 654 | 1 | 639 | beta-fructosidase [Arabidopsis thaliana] |
| RefSeq | NP_564798.1 | 0 | 1 | 654 | 1 | 648 | beta-fructosidase (BFRUCT3) / beta-fructofuranosidase / invertase, vacuolar [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 101 | 643 | 8 | 544 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 3ugh_A | 0 | 101 | 643 | 8 | 544 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 3ugg_B | 0 | 101 | 643 | 8 | 544 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 3ugg_A | 0 | 101 | 643 | 8 | 544 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 3ugf_B | 0 | 101 | 643 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
