y
Basic Information | |
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Species | Arabidopsis lyrata |
Cazyme ID | 888545 |
Family | GH32 |
Protein Properties | Length: 664 Molecular Weight: 73835.4 Isoelectric Point: 5.8278 |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 124 | 444 | 0 |
HFQPEQNWMNDPNGPLFYKGWYHFFYQYNPNAAVWGDIVWGHAVSRDLIHWVHLPIAMVADQWYDSNGVWTGSATILPDGSIVMLYTGSTDKAVQVQNLA YPEDPSDPLLLKWVKFPGNPVLVPPPGILPKDFRDPTTAWKTSEGKWRITIGSKLNRTGISLVYDTTDFKTYEKLDTLLHRVPNTGMWECVDFYPVSKTA VNGLDTSVKGPDVKHIVKASMDDTRFDHYAVGTYFDSNGTWIPDDPTIDVGMSTSLRYDYGKFYASKTFYDQNKGRRVLWSWIGESDSEAADVQKGWSSV QGIPRTVVLDTKTGKNLVQWP |
Full Sequence |
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Protein Sequence Length: 664 Download |
MASSDALLPI SAREEEPLCP YTRLPMADPN QETHGRRRRP FKGLLAVSFG LLFIAFYVAL 60 IATHDGSRSN DAGIDETETI TSRARLAGVS EKSNDGLWKL SGDRNTPAFE WNNSMLSWQR 120 TAFHFQPEQN WMNDPNGPLF YKGWYHFFYQ YNPNAAVWGD IVWGHAVSRD LIHWVHLPIA 180 MVADQWYDSN GVWTGSATIL PDGSIVMLYT GSTDKAVQVQ NLAYPEDPSD PLLLKWVKFP 240 GNPVLVPPPG ILPKDFRDPT TAWKTSEGKW RITIGSKLNR TGISLVYDTT DFKTYEKLDT 300 LLHRVPNTGM WECVDFYPVS KTAVNGLDTS VKGPDVKHIV KASMDDTRFD HYAVGTYFDS 360 NGTWIPDDPT IDVGMSTSLR YDYGKFYASK TFYDQNKGRR VLWSWIGESD SEAADVQKGW 420 SSVQGIPRTV VLDTKTGKNL VQWPVEEIKS LRLSSKQFDI KVGPGSVVPV DVGSAAQLDI 480 EAEFEINKES LDKILGNASV MAEAEEFSCE KSGGSTVRGA LGPFGFSVLA TESLSEQTPV 540 YFYVAKGKDS ELKTFFCIDT SRSSVANDVV KLIYGSVVPV LKGEKLTMRI LVDHSIVEAF 600 GQGGRTCITS RVYPTSAIYG AAKLFLFNNA LDATITASFR VWQMNSAFIH PYTEEAFRAL 660 TRT* 720 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 2.0e-56 | 120 | 614 | 513 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 1.0e-73 | 120 | 615 | 512 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 2.0e-95 | 130 | 447 | 324 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 1.0e-154 | 124 | 444 | 329 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 0 | 124 | 604 | 491 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAG36942.1 | 0 | 1 | 663 | 1 | 663 | AF274298_1 acid invertase [Brassica oleracea] |
GenBank | AAG36943.1 | 0 | 1 | 663 | 1 | 662 | AF274299_1 acid invertase [Brassica oleracea] |
EMBL | CAA66330.1 | 0 | 5 | 663 | 1 | 660 | beta-fructosidase [Arabidopsis thaliana] |
RefSeq | NP_563901.1 | 0 | 1 | 663 | 1 | 664 | ATBETAFRUCT4; beta-fructofuranosidase/ hydrolase, hydrolyzing O-glycosyl compounds [Arabidopsis thaliana] |
RefSeq | NP_564798.1 | 0 | 1 | 655 | 1 | 640 | beta-fructosidase (BFRUCT3) / beta-fructofuranosidase / invertase, vacuolar [Arabidopsis thaliana] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 105 | 652 | 4 | 544 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
PDB | 3ugh_A | 0 | 105 | 652 | 4 | 544 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
PDB | 3ugg_B | 0 | 105 | 652 | 4 | 544 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
PDB | 3ugg_A | 0 | 105 | 652 | 4 | 544 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
PDB | 3ugf_B | 0 | 105 | 652 | 4 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |