y
Basic Information | |
---|---|
Species | Arabidopsis lyrata |
Cazyme ID | 909139 |
Family | GH31 |
Protein Properties | Length: 906 Molecular Weight: 101451 Isoelectric Point: 5.7888 |
View CDS |
External Links |
---|
NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
---|---|---|---|
Family | Start | End | Evalue |
GH31 | 286 | 753 | 0 |
YVFTGPSPEMVMNQYTELIGRPAPMPYWSFGFHQCRYGYKNVSDLESVVDGYAKAGIPLEVMWTDIDYMDGYKDFTLDPVNFPEDKMKSFVDTLHKSGQK YVLILDPGIGVDSSYGTYNRGMEVDVFIKRNGEPYLGEVWPGKVYFPDFLNPAAATFWSNEIKMFQEILPLDGLWIDMNEISNFITSPLSSGSSLDDPPY KINNSGDKRPINNKTVPATSIHFGNISEYDVHNLYGLLEAKATHQAVVDITGKRPFILSRSTFVSSGKYTAHWTGDNAAKWEDLAYSIPGILNFGLFGIP MVGADICGFSHDTTEELCRRWIQLGAFYPFARDHSSLGTARQELYLWDSVASSARKVLGLRMRLLPHLYTLMYEAHISGNPIARPLFFSFPRDTKTYEID SQFLIGKNIMVSPALKQGTVAVDAYFPAGNWFDVFNYSFAVGGDSGKHVRLDTPADHVNVHVREGSIV |
Full Sequence |
---|
Protein Sequence Length: 906 Download |
MSSLHWFPNF FIVFVVFFSS HGSGSSQVVL EEEEATVVGY GYVVRSVAVD SNRQVLTAKL 60 DLIKPSSVYA PDIKSLSLHV SLETSERLRI RITDSSQQRW EIPETVIPRA GNHSPRRFLT 120 EEDGGNSSEN NFLADPSSDL VFTLHNTTPF GFSVSRRSSG DILFDTSPDQ SDSNTYFVFK 180 DQFLQLSSAL PENRSNLYGL GEHTKRSFKL ISGDTMTLWN ADIGSENPDV NLYGSHPFYM 240 DVRGSNGHEE AGTTHGVLLL NSNGMDVKYE GHRITYNVIG GVIDLYVFTG PSPEMVMNQY 300 TELIGRPAPM PYWSFGFHQC RYGYKNVSDL ESVVDGYAKA GIPLEVMWTD IDYMDGYKDF 360 TLDPVNFPED KMKSFVDTLH KSGQKYVLIL DPGIGVDSSY GTYNRGMEVD VFIKRNGEPY 420 LGEVWPGKVY FPDFLNPAAA TFWSNEIKMF QEILPLDGLW IDMNEISNFI TSPLSSGSSL 480 DDPPYKINNS GDKRPINNKT VPATSIHFGN ISEYDVHNLY GLLEAKATHQ AVVDITGKRP 540 FILSRSTFVS SGKYTAHWTG DNAAKWEDLA YSIPGILNFG LFGIPMVGAD ICGFSHDTTE 600 ELCRRWIQLG AFYPFARDHS SLGTARQELY LWDSVASSAR KVLGLRMRLL PHLYTLMYEA 660 HISGNPIARP LFFSFPRDTK TYEIDSQFLI GKNIMVSPAL KQGTVAVDAY FPAGNWFDVF 720 NYSFAVGGDS GKHVRLDTPA DHVNVHVREG SIVAMQGEAL TTRDARKTPY QLLVVASRLE 780 NISGELFLDE GENVRMGAGG GNRDWTLVKF RCFVTGKSVV LRSEVVNPEY ASKMKWSIEK 840 VTFVGFENVE SVKTYEVRTS ERLRSPRISL IKTVLDNDDP RFLSVEVSKL SLLVGKKFEM 900 RLRLT* 960 |
Functional Domains Download unfiltered results here | ||||||||
---|---|---|---|---|---|---|---|---|
Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd06600 | GH31_MGAM-like | 1.0e-112 | 305 | 664 | 363 | + This family includes the following closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), lysosomal acid alpha-glucosidase (GAA), neutral alpha-glucosidase C (GANC), the alpha subunit of neutral alpha-glucosidase AB (GANAB), and alpha-glucosidase II. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. Alpha-glucosidase II is a GH31 enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. The MGAM-like family corresponds to subgroup 1 in the Ernst et al classification of GH31 enzymes. | ||
cd06604 | GH31_glucosidase_II_MalA | 2.0e-124 | 305 | 664 | 366 | + Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. | ||
COG1501 | COG1501 | 5.0e-130 | 149 | 792 | 655 | + Alpha-glucosidases, family 31 of glycosyl hydrolases [Carbohydrate transport and metabolism] | ||
pfam01055 | Glyco_hydro_31 | 0 | 286 | 753 | 472 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
cd06602 | GH31_MGAM_SI_GAA | 0 | 305 | 682 | 383 | + This family includes the following three closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), and lysosomal acid alpha-glucosidase (GAA), also known as acid-maltase. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end, and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). |
Gene Ontology | |
---|---|
GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
Annotations - NR Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAB82656.1 | 0 | 1 | 905 | 1 | 902 | alpha-glucosidase 1 [Arabidopsis thaliana] |
RefSeq | NP_196733.1 | 0 | 1 | 905 | 1 | 902 | alpha-glucosidase 1 (AGLU1) [Arabidopsis thaliana] |
RefSeq | XP_002317678.1 | 0 | 37 | 904 | 46 | 912 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002317679.1 | 0 | 5 | 905 | 17 | 905 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002333838.1 | 0 | 32 | 905 | 27 | 896 | predicted protein [Populus trichocarpa] |
Annotations - PDB Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3w38_A | 0 | 32 | 904 | 35 | 908 | A Chain A, The Structure Of A Protein In Glycosyl Transferase Family 8 From Anaerococcus Prevotii. |
PDB | 3w37_A | 0 | 32 | 904 | 35 | 908 | A Chain A, The Structure Of A Protein In Glycosyl Transferase Family 8 From Anaerococcus Prevotii. |
PDB | 3ctt_A | 0 | 40 | 792 | 50 | 774 | A Chain A, The Structure Of A Protein In Glycosyl Transferase Family 8 From Anaerococcus Prevotii. |
PDB | 2qmj_A | 0 | 40 | 792 | 50 | 774 | A Chain A, The Structure Of A Protein In Glycosyl Transferase Family 8 From Anaerococcus Prevotii. |
PDB | 2qly_A | 0 | 40 | 792 | 50 | 774 | A Chain A, Crystral Structure Of The N-Terminal Subunit Of Human Maltase- Glucoamylase |