Basic Information | |
---|---|
Species | Arabidopsis thaliana |
Cazyme ID | AT1G12240.1 |
Family | GH32 |
Protein Properties | Length: 665 Molecular Weight: 73844.2 Isoelectric Point: 5.3023 |
Chromosome | Chromosome/Scaffold: 1 Start: 4153652 End: 4157644 |
Description | glycosyl hydrolases, putative, expressed |
View CDS |
Signature Domain Download full data set without filtering | |||
---|---|---|---|
Family | Start | End | Evalue |
GH32 | 125 | 445 | 0 |
HFQPEQNWMNDPNGPLFYKGWYHFFYQYNPNAAVWGDIVWGHAVSRDLIHWVHLPIAMVADQWYDSNGVWTGSATFLPDGSIVMLYTGSTDKAVQVQNLA YPEDPNDPLLLKWVKFPGNPVLVPPPGILPKDFRDPTTAWKTSEGKWRITIGSKLNKTGISLVYDTIDFKTYEKLDTLLHRVPNTGMWECVDFYPVSKTA GNGLDTSVNGPDVKHIVKASMDDTRFDHYAVGTYFDSNGTWIPDDPTIDVGMTASLRYDYGKFYASKSFYDQNKGRRVLWSWIGESDSEASDVQKGWSSL QGIPRTVVLDTKTGKNLVQWP |
Full Sequence |
---|
Protein Sequence Length: 665 Download |
MASSDALLPI SAREEEPLCP YTRLPMADPN QETHGPRRRR PFKGLLAVSF GLLFIAFYVA 60 LIATHDGSRS NDEGIDETET ITSRARLAGV SEKRNDGLWK LSGDRNTPAF EWNNSMLSWQ 120 RTAFHFQPEQ NWMNDPNGPL FYKGWYHFFY QYNPNAAVWG DIVWGHAVSR DLIHWVHLPI 180 AMVADQWYDS NGVWTGSATF LPDGSIVMLY TGSTDKAVQV QNLAYPEDPN DPLLLKWVKF 240 PGNPVLVPPP GILPKDFRDP TTAWKTSEGK WRITIGSKLN KTGISLVYDT IDFKTYEKLD 300 TLLHRVPNTG MWECVDFYPV SKTAGNGLDT SVNGPDVKHI VKASMDDTRF DHYAVGTYFD 360 SNGTWIPDDP TIDVGMTASL RYDYGKFYAS KSFYDQNKGR RVLWSWIGES DSEASDVQKG 420 WSSLQGIPRT VVLDTKTGKN LVQWPVEEIK SLRLSSKQFD LEVGPGSVVP VDVGSAAQLD 480 IEAEFEINKE SLDKIIGNAS VVAEAEEFSC EKSGGSTVRG ALGPFGFSVL ATESLSEQTP 540 VYFYVAKGKD SELKTFFCTD TSRSSVANDV VKPIYGSVVP VLKGEKLTMR ILVDHSIVEA 600 FGQGGRTCIT SRVYPTTAIY GAAKLFLFNN ALDATVTASF TVWQMNSAFI HPYSDEAVRA 660 LSRT* |
Functional Domains Download unfiltered results here | ||||||||
---|---|---|---|---|---|---|---|---|
Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 9.0e-56 | 121 | 615 | 511 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 4.0e-71 | 121 | 644 | 535 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 2.0e-93 | 131 | 448 | 324 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 3.0e-152 | 125 | 445 | 329 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 0 | 125 | 605 | 491 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
---|---|
GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
GO:0005773 | vacuole |
GO:0005975 | carbohydrate metabolic process |
Annotations - NR Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAG36942.1 | 0 | 1 | 664 | 1 | 663 | AF274298_1 acid invertase [Brassica oleracea] |
GenBank | AAG36943.1 | 0 | 1 | 664 | 1 | 662 | AF274299_1 acid invertase [Brassica oleracea] |
EMBL | CAA66330.1 | 0 | 5 | 664 | 1 | 660 | beta-fructosidase [Arabidopsis thaliana] |
RefSeq | NP_563901.1 | 0 | 1 | 664 | 1 | 664 | ATBETAFRUCT4; beta-fructofuranosidase/ hydrolase, hydrolyzing O-glycosyl compounds [Arabidopsis thaliana] |
RefSeq | NP_564798.1 | 0 | 1 | 664 | 1 | 648 | beta-fructosidase (BFRUCT3) / beta-fructofuranosidase / invertase, vacuolar [Arabidopsis thaliana] |
Annotations - PDB Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 106 | 653 | 4 | 544 | A Chain A, Crystal Structure At 1.45- Resolution Of The Major Allergen Endo-Beta-1,3-Glucanase Of Banana As A Molecular Basis For The Latex-Fruit Syndrome |
PDB | 3ugh_A | 0 | 106 | 653 | 4 | 544 | A Chain A, Crystal Structure At 1.45- Resolution Of The Major Allergen Endo-Beta-1,3-Glucanase Of Banana As A Molecular Basis For The Latex-Fruit Syndrome |
PDB | 3ugg_B | 0 | 106 | 653 | 4 | 544 | A Chain A, Crystal Structure At 1.45- Resolution Of The Major Allergen Endo-Beta-1,3-Glucanase Of Banana As A Molecular Basis For The Latex-Fruit Syndrome |
PDB | 3ugg_A | 0 | 106 | 653 | 4 | 544 | A Chain A, Crystal Structure At 1.45- Resolution Of The Major Allergen Endo-Beta-1,3-Glucanase Of Banana As A Molecular Basis For The Latex-Fruit Syndrome |
PDB | 3ugf_B | 0 | 106 | 653 | 4 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
Metabolic Pathways | |||
---|---|---|---|
Pathway Name | Reaction | EC | Protein Name |
fructan biosynthesis | RXN-1781 | EC-2.4.1.99 | sucrose:sucrose fructosyltransferase |
sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |