| Basic Information | |
|---|---|
| Species | Arabidopsis thaliana |
| Cazyme ID | AT1G14185.1 |
| Family | AA3 |
| Protein Properties | Length: 504 Molecular Weight: 55420.2 Isoelectric Point: 8.3213 |
| Chromosome | Chromosome/Scaffold: 1 Start: 4850274 End: 4852096 |
| Description | HOTHEAD precursor, putative, expressed |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA3 | 33 | 501 | 0 |
| SGKSFDYIVVGGGTAGCSLAATLSEKYSVLVIERGGSPFGDPLVEDKKYYGYSLINTDEYSSVAQSFTSVDGIKNHRGRVLGGSSAINGGFYSRASDEFV KKAGWDKDLVQESYKWVESKVVFMPELTRWQSIVQFGFLEAGFYPYNGYSLEHTQGTKIGGSIFDQCGKRHTSADLLGYGKPNCITVLLNATVKSIIFDA NKTRAVGVRFMESDGNSSKSYKVHVEQHRGEVILAAGALGSPQILLLSGIGPENHLNDFDIPVIVNLKEVGKQMSDNPAISLLVDRFSQNLTVDPPQVVA ITEGFKFILQSLVLPTNITTTRTAISAKIAFPKSKGRLKLNNTNPRENPSVTFNYLENKADLDACQEMVLHLQHVARSKTVTFFLGTQAQDKLVAGDEEL KKFCIKNVRTYYHYHGGCVVGSVVNEEYKVNGVKRLRVVDGSTFEESPGTNPMATVLMLGRYQGIKILK | |||
| Full Sequence |
|---|
| Protein Sequence Length: 504 Download |
| MSHFVFLFII SMFINLSQGA QMPYMTTDPK EVSGKSFDYI VVGGGTAGCS LAATLSEKYS 60 VLVIERGGSP FGDPLVEDKK YYGYSLINTD EYSSVAQSFT SVDGIKNHRG RVLGGSSAIN 120 GGFYSRASDE FVKKAGWDKD LVQESYKWVE SKVVFMPELT RWQSIVQFGF LEAGFYPYNG 180 YSLEHTQGTK IGGSIFDQCG KRHTSADLLG YGKPNCITVL LNATVKSIIF DANKTRAVGV 240 RFMESDGNSS KSYKVHVEQH RGEVILAAGA LGSPQILLLS GIGPENHLND FDIPVIVNLK 300 EVGKQMSDNP AISLLVDRFS QNLTVDPPQV VAITEGFKFI LQSLVLPTNI TTTRTAISAK 360 IAFPKSKGRL KLNNTNPREN PSVTFNYLEN KADLDACQEM VLHLQHVARS KTVTFFLGTQ 420 AQDKLVAGDE ELKKFCIKNV RTYYHYHGGC VVGSVVNEEY KVNGVKRLRV VDGSTFEESP 480 GTNPMATVLM LGRYQGIKIL KEH* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PRK02106 | PRK02106 | 1.0e-37 | 35 | 308 | 323 | + choline dehydrogenase; Validated | ||
| TIGR03970 | Rv0697 | 2.0e-42 | 38 | 492 | 510 | + dehydrogenase, Rv0697 family. This model describes a set of dehydrogenases belonging to the glucose-methanol-choline oxidoreductase (GMC oxidoreductase) family. Members of the present family are restricted to Actinobacterial genome contexts containing also members of families TIGR03962 and TIGR03969 (the mycofactocin system), and are proposed to be uniform in function. | ||
| TIGR01810 | betA | 3.0e-45 | 38 | 492 | 547 | + choline dehydrogenase. Choline dehydrogenase catalyzes the conversion of exogenously supplied choline into the intermediate glycine betaine aldehyde, as part of a two-step oxidative reaction leading to the formation of osmoprotectant betaine. This enzymatic system can be found in both gram-positive and gram-negative bacteria. As in Escherichia coli , Staphylococcus xylosus , and Sinorhizobium meliloti, this enzyme is found associated in a transciptionally co-induced gene cluster with betaine aldehyde dehydrogenase, the second catalytic enzyme in this reaction. Other gram-positive organisms have been shown to employ a different enzymatic system, utlizing a soluable choline oxidase or type III alcohol dehydrogenase instead of choline dehydrogenase. This enzyme is a member of the GMC oxidoreductase family (pfam00732 and pfam05199), sharing a common evoluntionary origin and enzymatic reaction with alcohol dehydrogenase. Outgrouping from this model, Caulobacter crescentus shares sequence homology with choline dehydrogenase, yet other genes participating in this enzymatic reaction have not currently been identified [Cellular processes, Adaptations to atypical conditions]. | ||
| COG2303 | BetA | 9.0e-52 | 30 | 503 | 555 | + Choline dehydrogenase and related flavoproteins [Amino acid transport and metabolism] | ||
| PLN02785 | PLN02785 | 4.0e-162 | 36 | 502 | 534 | + Protein HOTHEAD | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0006066 | alcohol metabolic process |
| GO:0012505 | endomembrane system |
| GO:0016614 | oxidoreductase activity, acting on CH-OH group of donors |
| GO:0016832 | aldehyde-lyase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | NP_172871.1 | 0 | 22 | 503 | 1 | 483 | glucose-methanol-choline (GMC) oxidoreductase family protein [Arabidopsis thaliana] |
| RefSeq | NP_563939.1 | 0 | 1 | 503 | 1 | 503 | glucose-methanol-choline (GMC) oxidoreductase family protein [Arabidopsis thaliana] |
| RefSeq | XP_002312249.1 | 0 | 25 | 503 | 1 | 487 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002315047.1 | 0 | 19 | 502 | 1 | 489 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002522448.1 | 0 | 3 | 503 | 10 | 525 | glucose-methanol-choline (gmc) oxidoreductase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3red_L | 0 | 36 | 502 | 26 | 517 | A Chain A, 3.0 A Structure Of The Prunus Mume Hydroxynitrile Lyase Isozyme-1 |
| PDB | 3red_K | 0 | 36 | 502 | 26 | 517 | A Chain A, 3.0 A Structure Of The Prunus Mume Hydroxynitrile Lyase Isozyme-1 |
| PDB | 3red_J | 0 | 36 | 502 | 26 | 517 | A Chain A, 3.0 A Structure Of The Prunus Mume Hydroxynitrile Lyase Isozyme-1 |
| PDB | 3red_I | 0 | 36 | 502 | 26 | 517 | A Chain A, 3.0 A Structure Of The Prunus Mume Hydroxynitrile Lyase Isozyme-1 |
| PDB | 3red_H | 0 | 36 | 502 | 26 | 517 | A Chain A, 3.0 A Structure Of The Prunus Mume Hydroxynitrile Lyase Isozyme-1 |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cutin biosynthesis | RXN-2121 | EC-1.1.1 | 18-hydroxyoleate dehydrogenase |
| cutin biosynthesis | RXN-9802 | EC-1.1.1 | 16-hydroxypalmitate dehydrogenase |
| suberin biosynthesis | RXN-2121 | EC-1.1.1 | 18-hydroxyoleate dehydrogenase |
