| Basic Information | |
|---|---|
| Species | Arabidopsis thaliana |
| Cazyme ID | AT1G33700.2 |
| Family | GH116 |
| Protein Properties | Length: 948 Molecular Weight: 105750 Isoelectric Point: 6.6885 |
| Chromosome | Chromosome/Scaffold: 1 Start: 12208308 End: 12214182 |
| Description | non-lysosomal glucosylceramidase, putative, expressed |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
 | |||
| Family | Start | End | Evalue |
| GH116 | 520 | 881 | 0 |
| GQFLYLEGVQYLMYNTYDVHFYSSFALLMLFPKIELSIQRDFAAAVLMHDSSKKQVMSSGEFVTRKVLGAVPHDIGLNDPWFELNAYNLFNTDRWKDLNS KFVLQVYRDVVATGDLNFAKAVWPSVYTAIAYLDQFDKDGDGMIENEGFPDQTYDAWSCSGVSAYCGGLWVAALQAGSALAREIGDNGAAVYFNAKYEKA RSVYEKLWNGSYFNYDNSRSGSSSSILADQMAGQWYARACGLKPIAKEEWIKKALETVYDFNVMRVRDGTRGAVNGMLPDGRVDTSTMVSREVWAGTTYS VAACMIQEGLADKGFRTASGIYEAAWSDRGLGCAFQTPEAWTTNDEYRSLCYMRPLAIWGIQ | |||
| Full Sequence |
|---|
| Protein Sequence Length: 948 Download |
| MQNVPEDQNK MVIDDKLPPF SWERKLNSQA KTPSEFKLSK RDHMHLFPLG YRLWRHTKDE 60 AAKGRASIFD IFRKHHITGD HGVPLGGIGS GSIGRSYKGE FQQFKLFPKI CEEAPILTNQ 120 FSAFVSRPGG VKHSTVLCPS KPQVVKDNGG YLCQGQAPNM GIESWDWNMT GEKSTYHALY 180 PRSWTVYDGE PDPELRIVSR QVSPFIPHNY EESSLPVSVF NFTVTNTGAE PAIVTLLFTW 240 ENSVGGASGL TGQHFNSTMK AKDGVHAVAL QHKTANGHPP VSYAIAAKET EDVRVSSCPC 300 FIVSGTTPNQ ITAGDMWDEI KKNASFDKLT SNACSPSKPG TSIGAAIAAK VKVPPGCDRT 360 VTFSLSWDCP EARFDEKTYH RRYTRFYGSL GNAAVAMAHD ALLNFPEWET QIEEWQAPVL 420 ADTTLPEWYR VTLFNELYYF NSGGTMWTDG LPPKQSLDSI GRRKISLGLS TIDKNDQDQN 480 NVALDILGRI DAVCSQIHAP LTSNAALGNT MIQNTTENIG QFLYLEGVQY LMYNTYDVHF 540 YSSFALLMLF PKIELSIQRD FAAAVLMHDS SKKQVMSSGE FVTRKVLGAV PHDIGLNDPW 600 FELNAYNLFN TDRWKDLNSK FVLQVYRDVV ATGDLNFAKA VWPSVYTAIA YLDQFDKDGD 660 GMIENEGFPD QTYDAWSCSG VSAYCGGLWV AALQAGSALA REIGDNGAAV YFNAKYEKAR 720 SVYEKLWNGS YFNYDNSRSG SSSSILADQM AGQWYARACG LKPIAKEEWI KKALETVYDF 780 NVMRVRDGTR GAVNGMLPDG RVDTSTMVSR EVWAGTTYSV AACMIQEGLA DKGFRTASGI 840 YEAAWSDRGL GCAFQTPEAW TTNDEYRSLC YMRPLAIWGI QWAHTMPKPN REQEVSLRPQ 900 EEDATSVLFQ QHAGFIKVAH YLKNTKGKDH RNRLQTAYET FLKTIRL* |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
 | ||||||||
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG3408 | GDB1 | 0.0004 | 588 | 736 | 163 | + Glycogen debranching enzyme [Carbohydrate transport and metabolism] | ||
| COG4354 | COG4354 | 2.0e-26 | 145 | 449 | 317 | + Predicted bile acid beta-glucosidase [Carbohydrate transport and metabolism] | ||
| pfam12215 | GBA2_N | 2.0e-59 | 82 | 408 | 340 | + beta-Glucocerebrosidase 2 N terminal. This domain is found in bacteria, archaea and eukaryotes. This domain is typically between 320 to 354 amino acids in length. This domain is found associated with pfam04685. This domain is found in the protein beta-Glucocerebrosidase 2. It is found just after the extreme N terminus. This protein is located in the ER. The N terminal is thought to be the luminal domain while the C terminal is the cytosolic domain. The catalytic domain of GBA-2 is unknown. | ||
| COG4354 | COG4354 | 3.0e-71 | 506 | 880 | 398 | + Predicted bile acid beta-glucosidase [Carbohydrate transport and metabolism] | ||
| pfam04685 | DUF608 | 2.0e-170 | 520 | 882 | 367 | + Protein of unknown function, DUF608. This family represents a conserved region with a pankaryotic distribution in a number of uncharacterized proteins. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004348 | glucosylceramidase activity |
| GO:0005773 | vacuole |
| GO:0006665 | sphingolipid metabolic process |
| GO:0006680 | glucosylceramide catabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAF97289.1 | 0 | 123 | 947 | 23 | 790 | AC010164_11 Hypothetical protein [Arabidopsis thaliana] |
| GenBank | AAM19831.1 | 0 | 6 | 947 | 10 | 922 | AT4g10060/T5L19_190 [Arabidopsis thaliana] |
| GenBank | AAO42222.1 | 0 | 1 | 947 | 1 | 947 | unknown protein [Arabidopsis thaliana] |
| RefSeq | NP_174631.2 | 0 | 1 | 947 | 1 | 947 | catalytic/ glucosylceramidase [Arabidopsis thaliana] |
| RefSeq | NP_192744.6 | 0 | 6 | 947 | 10 | 922 | catalytic/ glucosylceramidase [Arabidopsis thaliana] |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
 | ||||
| Hit | Length | Start | End | EValue |
| DY292040 | 366 | 533 | 895 | 0 |
| JG703027 | 328 | 538 | 865 | 0 |
| FC917648 | 306 | 533 | 838 | 0 |
| FC895322 | 303 | 397 | 695 | 0 |
| DY268096 | 310 | 431 | 736 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
