| Basic Information | |
|---|---|
| Species | Arabidopsis thaliana |
| Cazyme ID | AT1G34575.1 |
| Family | AA7 |
| Protein Properties | Length: 528 Molecular Weight: 59054 Isoelectric Point: 9.4116 |
| Chromosome | Chromosome/Scaffold: 1 Start: 12657149 End: 12658732 |
| Description | reticuline oxidase-like protein precursor, putative, expressed |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 67 | 521 | 0 |
| NLRYFNGTTRKPVAIVAAAHFTHIQATINCAKKLGLQLRIRSGGHDYDGMSYLSTVDFVVLDMFNLRAIEIDPKLDTAWVQSGATLGEIYYNVANKSNNL RGFPAGICPGLGAGGHFSGGGYGNMMRKYGLSIDNIIDAKIVDANARVLDRSSMGEDLFWALRGGGAASFCVVLAWKIKLVPVPEKVTVFNVETIGNRGV IPTDLAAKWQEIADKIDNDLFIRLTLSSSNKTVKASFMGMYLGNSEKLLEIMNAKFPELGLNKTECIEMKWIESVLFWLSIPPGTAPTSVMLNRIPQKQI YLKRKSDYVQKPISKPGLESIFKILSENENVSMAWNPYGGRMSEIPATETAFPHRAGNMFKIQYSSNWFVPGEEAASDCLSQTERVFEAMSPYVSKNPRE AFLNYRDIDIGKNLNSTYEEGKVYGVKYFKNNFERLVQVKTRVDPDNIFRYEQSI | |||
| Full Sequence |
|---|
| Protein Sequence Length: 528 Download |
| MKSSTTQTLI FTVFLLLIPT SFAAPPKLKD SFTQCVTVFK PSVPIQNFTY TQQNPNFLTI 60 LNNYVRNLRY FNGTTRKPVA IVAAAHFTHI QATINCAKKL GLQLRIRSGG HDYDGMSYLS 120 TVDFVVLDMF NLRAIEIDPK LDTAWVQSGA TLGEIYYNVA NKSNNLRGFP AGICPGLGAG 180 GHFSGGGYGN MMRKYGLSID NIIDAKIVDA NARVLDRSSM GEDLFWALRG GGAASFCVVL 240 AWKIKLVPVP EKVTVFNVET IGNRGVIPTD LAAKWQEIAD KIDNDLFIRL TLSSSNKTVK 300 ASFMGMYLGN SEKLLEIMNA KFPELGLNKT ECIEMKWIES VLFWLSIPPG TAPTSVMLNR 360 IPQKQIYLKR KSDYVQKPIS KPGLESIFKI LSENENVSMA WNPYGGRMSE IPATETAFPH 420 RAGNMFKIQY SSNWFVPGEE AASDCLSQTE RVFEAMSPYV SKNPREAFLN YRDIDIGKNL 480 NSTYEEGKVY GVKYFKNNFE RLVQVKTRVD PDNIFRYEQS IPVHVSR* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG0277 | GlcD | 6.0e-13 | 61 | 283 | 235 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam08031 | BBE | 1.0e-16 | 467 | 522 | 56 | + Berberine and berberine like. This domain is found in the berberine bridge and berberine bridge- like enzymes which are involved in the biosynthesis of numerous isoquinoline alkaloids. They catalyze the transformation of the N-methyl group of (S)-reticuline into the C-8 berberine bridge carbon of (S)-scoulerine. | ||
| pfam01565 | FAD_binding_4 | 9.0e-19 | 78 | 218 | 142 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009055 | electron carrier activity |
| GO:0012505 | endomembrane system |
| GO:0016491 | oxidoreductase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAF79255.1 | 0 | 1 | 527 | 1 | 527 | AC023279_4 F12K21.9 [Arabidopsis thaliana] |
| RefSeq | NP_174358.1 | 0 | 1 | 527 | 1 | 531 | FAD-binding domain-containing protein [Arabidopsis thaliana] |
| RefSeq | NP_564449.1 | 0 | 1 | 527 | 1 | 527 | FAD-binding domain-containing protein [Arabidopsis thaliana] |
| RefSeq | XP_002317086.1 | 0 | 15 | 526 | 10 | 528 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002523155.1 | 0 | 11 | 524 | 9 | 524 | Reticuline oxidase precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 4dns_B | 0 | 23 | 522 | 1 | 494 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 4dns_A | 0 | 23 | 522 | 1 | 494 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 3vte_A | 0 | 29 | 522 | 4 | 511 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| PDB | 3tsj_B | 0 | 25 | 522 | 4 | 494 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| PDB | 3tsj_A | 0 | 25 | 522 | 4 | 494 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cannabinoid biosynthesis | RXN-7854 | EC-1.21.3 | tetrahydrocannabinolic acid synthase |
