| Basic Information | |
|---|---|
| Species | Arabidopsis thaliana |
| Cazyme ID | AT1G49660.1 |
| Family | CE10 |
| Protein Properties | Length: 320 Molecular Weight: 35196.9 Isoelectric Point: 5.242 |
| Chromosome | Chromosome/Scaffold: 1 Start: 18378697 End: 18379799 |
| Description | CXE carboxylesterase, putative, expressed |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CE10 | 35 | 317 | 0 |
| DPTYDVVSKDVIYSPENNLSVRLFLPHKSTKLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPVPAAYEDVWSAIQW IFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKKLDLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDD PLFNVNGSGSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNPKSDKALKFLKKFVEFI | |||
| Full Sequence |
|---|
| Protein Sequence Length: 320 Download |
| MESEIASEFL PFCRIYKDGR VERLIGTDTI PASLDPTYDV VSKDVIYSPE NNLSVRLFLP 60 HKSTKLTAGN KLPLLIYIHG GAWIIESPFS PLYHNYLTEV VKSANCLAVS VQYRRAPEDP 120 VPAAYEDVWS AIQWIFAHSN GSGPVDWINK HADFGKVFLG GDSAGGNISH HMAMKAGKEK 180 KLDLKIKGIA VVHPAFWGTD PVDEYDVQDK ETRSGIAEIW EKIASPNSVN GTDDPLFNVN 240 GSGSDFSGLG CDKVLVAVAG KDVFVRQGLA YAAKLEKCEW EGTVEVVEEE GEDHVFHLQN 300 PKSDKALKFL KKFVEFIIG* 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00135 | COesterase | 1.0e-5 | 46 | 178 | 147 | + Carboxylesterase family. | ||
| COG2272 | PnbA | 1.0e-7 | 31 | 166 | 147 | + Carboxylesterase type B [Lipid metabolism] | ||
| cd00312 | Esterase_lipase | 4.0e-8 | 35 | 178 | 158 | + Esterases and lipases (includes fungal lipases, cholinesterases, etc.) These enzymes act on carboxylic esters (EC: 3.1.1.-). The catalytic apparatus involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine.These catalytic residues are responsible for the nucleophilic attack on the carbonyl carbon atom of the ester bond. In contrast with other alpha/beta hydrolase fold family members, p-nitrobenzyl esterase and acetylcholine esterase have a Glu instead of Asp at the active site carboxylate. | ||
| COG0657 | Aes | 2.0e-27 | 55 | 314 | 268 | + Esterase/lipase [Lipid metabolism] | ||
| pfam07859 | Abhydrolase_3 | 1.0e-55 | 75 | 298 | 230 | + alpha/beta hydrolase fold. This catalytic domain is found in a very wide range of enzymes. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004091 | carboxylesterase activity |
| GO:0005575 | cellular_component |
| GO:0008152 | metabolic process |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAM61628.1 | 0 | 11 | 319 | 67 | 374 | putative esterase [Arabidopsis thaliana] |
| GenBank | AAT68324.1 | 0 | 1 | 318 | 1 | 314 | hypothetical protein At1g49640 [Arabidopsis thaliana] |
| RefSeq | NP_175387.1 | 0 | 1 | 318 | 1 | 314 | hydrolase [Arabidopsis thaliana] |
| RefSeq | NP_175389.1 | 0 | 1 | 319 | 1 | 319 | AtCXE5 (Arabidopsis thaliana carboxyesterase 5); carboxylesterase |
| RefSeq | NP_564550.1 | 0 | 11 | 319 | 67 | 374 | cell death associated protein-related [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2o7v_A | 4e-35 | 8 | 314 | 20 | 327 | A Chain A, Crystal Structure Of The C-Terminal Globular Domain Of Oligosaccharyltransferase (Af_0329) From Archaeoglobus Fulgidus |
| PDB | 2o7r_A | 4e-35 | 8 | 314 | 20 | 327 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 2zsi_A | 7e-26 | 30 | 281 | 60 | 315 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 2zsh_A | 7e-26 | 30 | 281 | 60 | 315 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| PDB | 3ed1_F | 3e-25 | 18 | 298 | 37 | 329 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| formononetin biosynthesis | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| formononetin biosynthesis | RXN-3625 | - | 2,7-dihydroxy-4'-methoxyisoflavanone dehydratase |
| isoflavonoid biosynthesis I | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-3303 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-5502 | - | 2,7,5-trihydroxy-4'-methoxyisoflavanone dehydratase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| DK458597 | 270 | 1 | 270 | 0 |
| EE438263 | 261 | 1 | 261 | 0 |
| DY023972 | 262 | 3 | 264 | 0 |
| GR719789 | 250 | 1 | 250 | 0 |
| FY446802 | 247 | 3 | 249 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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