| Basic Information | |
|---|---|
| Species | Arabidopsis thaliana |
| Cazyme ID | AT1G60450.1 |
| Family | GT8 |
| Protein Properties | Length: 333 Molecular Weight: 37768.3 Isoelectric Point: 4.6732 |
| Chromosome | Chromosome/Scaffold: 1 Start: 22271226 End: 22273214 |
| Description | glycosyl transferase 8 domain containing protein, putative, expressed |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT8 | 11 | 267 | 0 |
| NASEKAPKERAYVTFLAGNGDYVKGVVGLAKGLRKVKSAYPLVVAMLPDVPEEHREILRSQGCIVREIEPVHPPDSQDAYARAYYIINYSKLRIWNFEEY NKMIYLDADIQVFGNIDDLFDMQDGYLHGVLSCFCEKIWSYTPLYSIGYCQYCPEKVVWPAEMESAPPSPYFNAGMFVFEPNPLTYESLLQTLQVTPPTP FAEQDFLNMFFGKVFKPVSPVYNLILSVLWRHPGKVDLESVKVVHYCPPGSKPWRYT | |||
| Full Sequence |
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| Protein Sequence Length: 333 Download |
| MTPETHVDMI NASEKAPKER AYVTFLAGNG DYVKGVVGLA KGLRKVKSAY PLVVAMLPDV 60 PEEHREILRS QGCIVREIEP VHPPDSQDAY ARAYYIINYS KLRIWNFEEY NKMIYLDADI 120 QVFGNIDDLF DMQDGYLHGV LSCFCEKIWS YTPLYSIGYC QYCPEKVVWP AEMESAPPSP 180 YFNAGMFVFE PNPLTYESLL QTLQVTPPTP FAEQDFLNMF FGKVFKPVSP VYNLILSVLW 240 RHPGKVDLES VKVVHYCPPG SKPWRYTGEE PNMDREDVKM LIKKWWDIYN DESLDFKPKS 300 PADLEATVLE STIIASVTEA PLSYSPAAPS AA* 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00505 | Glyco_transf_8 | 3.0e-13 | 23 | 266 | 264 | + Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+. | ||
| cd04194 | GT8_A4GalT_like | 7.0e-15 | 59 | 266 | 239 | + A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS. | ||
| pfam01501 | Glyco_transf_8 | 6.0e-60 | 23 | 268 | 264 | + Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase. | ||
| cd02537 | GT8_Glycogenin | 5.0e-80 | 20 | 292 | 276 | + Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen. Glycogenin initiates the biosynthesis of glycogen by incorporating glucose residues through a self-glucosylation reaction at a Tyr residue, and then acts as substrate for chain elongation by glycogen synthase and branching enzyme. It contains a conserved DxD motif and an N-terminal beta-alpha-beta Rossmann-like fold that are common to the nucleotide-binding domains of most glycosyltransferases. The DxD motif is essential for coordination of the catalytic divalent cation, most commonly Mn2+. Glycogenin can be classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. It is placed in glycosyltransferase family 8 which includes lipopolysaccharide glucose and galactose transferases and galactinol synthases. | ||
| PLN00176 | PLN00176 | 0 | 1 | 319 | 322 | + galactinol synthase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0005575 | cellular_component |
| GO:0016051 | carbohydrate biosynthetic process |
| GO:0016757 | transferase activity, transferring glycosyl groups |
| GO:0016758 | transferase activity, transferring hexosyl groups |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAC24075.1 | 0 | 9 | 332 | 1 | 345 | Strong similarity to water stress-induced protein, WSI76 isolog T08I13.2 gb |
| GenBank | ABK93731.1 | 0 | 1 | 332 | 1 | 336 | unknown [Populus trichocarpa] |
| DDBJ | BAE99313.1 | 0 | 1 | 332 | 1 | 334 | hypothetical protein [Arabidopsis thaliana] |
| RefSeq | NP_176248.1 | 0 | 1 | 332 | 1 | 332 | AtGolS7 (Arabidopsis thaliana galactinol synthase 7); transferase, transferring glycosyl groups / transferase, transferring hexosyl groups |
| RefSeq | NP_176250.1 | 0 | 1 | 332 | 1 | 334 | AtGolS4 (Arabidopsis thaliana galactinol synthase 4); transferase, transferring glycosyl groups / transferase, transferring hexosyl groups |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3u2w_B | 4e-21 | 19 | 294 | 4 | 259 | PP Chain p, Structural Characterization Of An Lpa1 Second Extracellular Loop Mimetic With A Self-assembling Coiled-coil Folding Constraint |
| PDB | 3u2w_A | 4e-21 | 19 | 294 | 4 | 259 | PP Chain p, Structural Characterization Of An Lpa1 Second Extracellular Loop Mimetic With A Self-assembling Coiled-coil Folding Constraint |
| PDB | 3qvb_A | 4e-21 | 19 | 294 | 4 | 259 | PP Chain p, Structural Characterization Of An Lpa1 Second Extracellular Loop Mimetic With A Self-assembling Coiled-coil Folding Constraint |
| PDB | 3q4s_A | 4e-21 | 19 | 294 | 4 | 259 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1), Apo Form |
| PDB | 3u2x_B | 7e-21 | 19 | 294 | 4 | 259 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1), Apo Form |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CV236809 | 290 | 19 | 308 | 0 |
| GR883155 | 325 | 1 | 319 | 0 |
| CV276648 | 278 | 19 | 296 | 0 |
| CV237441 | 286 | 23 | 308 | 0 |
| CV236822 | 286 | 23 | 308 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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