y
Basic Information | |
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Species | Arabidopsis thaliana |
Cazyme ID | AT1G62660.1 |
Family | GH32 |
Protein Properties | Length: 649 Molecular Weight: 72228.3 Isoelectric Point: 5.2671 |
Chromosome | Chromosome/Scaffold: 1 Start: 23199718 End: 23203765 |
Description | glycosyl hydrolases, putative, expressed |
View CDS |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 110 | 430 | 0 |
HFQPEKNWMNDPNGPLFYKGWYHFFYQYNPNAAVWGDIVWGHAVSKDLIHWLYLPIAMVPDQWYDANGVWTGSATFLDDGSIVMLYTGSTDEFVQVQNLA YPEDPSDPLLLKWVKFSGNPVLVPPPGIGAKDFRDPTTAWKTSSGKWRITIGSKINRTGISLIYDTTDFKTYEKHETLLHQVPNTGMWECVDFYPVSKTQ LNGLDTSVNGPDVKHVIKASMDDTRIDHYAIGTYDDSNATWVPDNPSIDVGISTGLRYDYGKYYASKTFYDQNKGRRILWGWIGESDSEAADVQKGWSSV QGIPRTVVLDTRTHKNLVQWP |
Full Sequence |
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Protein Sequence Length: 649 Download |
MASTEALLPV TSLQDPLSES RSDQIPETRR RRPIKVHLAV YSGLLLIALY VTLIVTHDGS 60 KAEIATESRP RMAGVSEKSN DGVWISSDDG KVEAFPWNNT ILSWQRTAFH FQPEKNWMND 120 PNGPLFYKGW YHFFYQYNPN AAVWGDIVWG HAVSKDLIHW LYLPIAMVPD QWYDANGVWT 180 GSATFLDDGS IVMLYTGSTD EFVQVQNLAY PEDPSDPLLL KWVKFSGNPV LVPPPGIGAK 240 DFRDPTTAWK TSSGKWRITI GSKINRTGIS LIYDTTDFKT YEKHETLLHQ VPNTGMWECV 300 DFYPVSKTQL NGLDTSVNGP DVKHVIKASM DDTRIDHYAI GTYDDSNATW VPDNPSIDVG 360 ISTGLRYDYG KYYASKTFYD QNKGRRILWG WIGESDSEAA DVQKGWSSVQ GIPRTVVLDT 420 RTHKNLVQWP VEEIKSLRLS SKKFDMTIGP GTVVPVDVGS ATQLDIEAEF EIKTDDLKLF 480 FDDDSVEADN KFSCETNGGS TARGALGPFG FSVLADEGLS EQTPVYFYVT KGKHSKLNTV 540 FCTDTSRSTL ANDVVKPIYG SFVPVLKGEK LTMRILVDHS IVEGFAQGGR SCITSRVYPT 600 KAIYGATKLF LFNNAIDATV TASFTVWQMN NAFIHPYSSD DLGVPSST* 660 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 2.0e-53 | 106 | 599 | 512 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 4.0e-71 | 106 | 628 | 536 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 6.0e-96 | 116 | 433 | 324 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 5.0e-152 | 110 | 430 | 329 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 0 | 110 | 589 | 490 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
GO:0005773 | vacuole |
GO:0005975 | carbohydrate metabolic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAD43622.1 | 0 | 1 | 648 | 1 | 650 | AC005698_21 T3P18.21 [Arabidopsis thaliana] |
GenBank | AAF19535.1 | 0 | 1 | 643 | 1 | 645 | AC007190_3 F23N19.3 [Arabidopsis thaliana] |
GenBank | AAK62665.1 | 0 | 1 | 648 | 1 | 648 | At1g62660/F23N19_3 [Arabidopsis thaliana] |
EMBL | CAA64781.1 | 0 | 10 | 648 | 1 | 639 | beta-fructosidase [Arabidopsis thaliana] |
RefSeq | NP_564798.1 | 0 | 1 | 648 | 1 | 648 | beta-fructosidase (BFRUCT3) / beta-fructofuranosidase / invertase, vacuolar [Arabidopsis thaliana] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 95 | 637 | 8 | 544 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
PDB | 3ugh_A | 0 | 95 | 637 | 8 | 544 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
PDB | 3ugg_B | 0 | 95 | 637 | 8 | 544 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
PDB | 3ugg_A | 0 | 95 | 637 | 8 | 544 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
PDB | 3ugf_B | 0 | 95 | 637 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
fructan biosynthesis | RXN-1781 | EC-2.4.1.99 | sucrose:sucrose fructosyltransferase |
sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |