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Basic Information | |
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Species | Arabidopsis thaliana |
Cazyme ID | AT1G68560.1 |
Family | GH31 |
Protein Properties | Length: 916 Molecular Weight: 102399 Isoelectric Point: 6.7616 |
Chromosome | Chromosome/Scaffold: 1 Start: 25734182 End: 25738002 |
Description | glycosyl hydrolase, family 31, putative, expressed |
View CDS |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH31 | 263 | 755 | 0 |
FYFIAGPSPLNVVDQYTQLIGRPAPMPYWSLGFHQCRWGYHNLSVVEDVVDNYKKAKIPLDVIWNDDDHMDGHKDFTLNPVAYPRAKLLAFLDKIHKIGM KYIVINDPGIGVNASYGTFQRAMAADVFIKYEGKPFLAQVWPGPVYFPDFLNPKTVSWWGDEIKRFHDLVPIDGLWIDMNEVSNFCSGLCTIPEGKQCPS GEGPGWVCCLDCKNITKTRWDDPPYKINATGVVAPVGFKTIATSATHYNGVREYDAHSIYGFSETIATHKGLLNVQGKRPFILSRSTFVGSGQYAAHWTG DNQGTWQSLQVSISTMLNFGIFGVPMVGSDICGFYPQPTEELCNRWIEVGAFYPFSRDHANYYSPRQELYQWDTVADSARNALGMRYKILPFLYTLNYEA HMTGAPIARPLFFSFPEYTECYGNSRQFLLGSSFMISPVLEQGKTEVEALFPPGSWYHMFDMTQAVVSKNGKRVTLPAPLNFVNVHLYQNTIL |
Full Sequence |
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Protein Sequence Length: 916 Download |
MASSSSSLAF SLSLLLALIL CFSPTQSYKT IGKGYRLVSI EESPDGGFIG YLQVKQKNKI 60 YGSDITTLRL FVKHETDSRL RVHITDAKQQ RWEVPYNLLP REQPPQVGKV IGKSRKSPIT 120 VQEISGSELI FSYTTDPFTF AVKRRSNHET LFNTTSSLVF KDQYLEISTS LPKEASLYGL 180 GENSQANGIK LVPNEPYTLY TEDVSAINLN TDLYGSHPMY MDLRNVGGKA YAHAVLLLNS 240 NGMDVFYRGD SLTYKVIGGV FDFYFIAGPS PLNVVDQYTQ LIGRPAPMPY WSLGFHQCRW 300 GYHNLSVVED VVDNYKKAKI PLDVIWNDDD HMDGHKDFTL NPVAYPRAKL LAFLDKIHKI 360 GMKYIVINDP GIGVNASYGT FQRAMAADVF IKYEGKPFLA QVWPGPVYFP DFLNPKTVSW 420 WGDEIKRFHD LVPIDGLWID MNEVSNFCSG LCTIPEGKQC PSGEGPGWVC CLDCKNITKT 480 RWDDPPYKIN ATGVVAPVGF KTIATSATHY NGVREYDAHS IYGFSETIAT HKGLLNVQGK 540 RPFILSRSTF VGSGQYAAHW TGDNQGTWQS LQVSISTMLN FGIFGVPMVG SDICGFYPQP 600 TEELCNRWIE VGAFYPFSRD HANYYSPRQE LYQWDTVADS ARNALGMRYK ILPFLYTLNY 660 EAHMTGAPIA RPLFFSFPEY TECYGNSRQF LLGSSFMISP VLEQGKTEVE ALFPPGSWYH 720 MFDMTQAVVS KNGKRVTLPA PLNFVNVHLY QNTILPTQQG GLISKDARTT PFSLVIAFPA 780 GASEGYATGK LYLDEDELPE MKLGNGQSTY VDFYASVGNG TMKMWSQVKE GKFALSKGWV 840 IEKVSVLGLR GAGQVSEIQI NGSPMTKKIE VSSKEHTYVI GLEDEEENKS VMVEVRGLEM 900 LVGKDFNMSW KMGIN* 960 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
pfam01055 | Glyco_hydro_31 | 3.0e-85 | 264 | 450 | 187 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
cd06602 | GH31_MGAM_SI_GAA | 4.0e-89 | 516 | 684 | 170 | + This family includes the following three closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), and lysosomal acid alpha-glucosidase (GAA), also known as acid-maltase. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end, and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). | ||
cd06603 | GH31_GANC_GANAB_alpha | 3.0e-96 | 283 | 666 | 390 | + This family includes the closely related glycosyl hydrolase family 31 (GH31) isozymes, neutral alpha-glucosidase C (GANC) and the alpha subunit of heterodimeric neutral alpha-glucosidase AB (GANAB). Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. The GANC/GANAB family includes the alpha-glucosidase II (ModA) from Dictyostelium discoideum as well as the alpha-glucosidase II (GLS2, or ROT2 - Reversal of TOR2 lethality protein 2) from Saccharomyces cerevisiae. | ||
pfam01055 | Glyco_hydro_31 | 2.0e-105 | 508 | 755 | 252 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
cd06604 | GH31_glucosidase_II_MalA | 4.0e-113 | 283 | 666 | 390 | + Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005618 | cell wall |
GO:0005975 | carbohydrate metabolic process |
GO:0009044 | xylan 1,4-beta-xylosidase activity |
GO:0009505 | plant-type cell wall |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAD05539.1 | 0 | 9 | 915 | 1 | 907 | alpha-xylosidase precursor [Arabidopsis thaliana] |
RefSeq | NP_177023.1 | 0 | 1 | 915 | 1 | 915 | XYL1 (ALPHA-XYLOSIDASE 1); alpha-N-arabinofuranosidase/ hydrolase, hydrolyzing O-glycosyl compounds / xylan 1,4-beta-xylosidase [Arabidopsis thaliana] |
RefSeq | NP_190180.1 | 0 | 8 | 913 | 1 | 853 | alpha-xylosidase, putative [Arabidopsis thaliana] |
RefSeq | XP_002311455.1 | 0 | 31 | 915 | 15 | 909 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002531635.1 | 0 | 1 | 915 | 1 | 929 | alpha-glucosidase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3w38_A | 0 | 11 | 908 | 13 | 904 | A Chain A, Heme Ligand Mutant Of Recombinant Horseradish Peroxidase In Complex With Benzhydroxamic Acid |
PDB | 3w37_A | 0 | 11 | 908 | 13 | 904 | A Chain A, Heme Ligand Mutant Of Recombinant Horseradish Peroxidase In Complex With Benzhydroxamic Acid |
PDB | 3lpp_D | 0 | 60 | 910 | 105 | 898 | A Chain A, Heme Ligand Mutant Of Recombinant Horseradish Peroxidase In Complex With Benzhydroxamic Acid |
PDB | 3lpp_C | 0 | 60 | 910 | 105 | 898 | A Chain A, Heme Ligand Mutant Of Recombinant Horseradish Peroxidase In Complex With Benzhydroxamic Acid |
PDB | 3lpp_B | 0 | 60 | 910 | 105 | 898 | A Chain A, Heme Ligand Mutant Of Recombinant Horseradish Peroxidase In Complex With Benzhydroxamic Acid |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
(1,4)-β-xylan degradation | 3.2.1.37-RXN | EC-3.2.1.37 | xylan 1,4-beta-xylosidase |
starch degradation I | RXN-2141 | EC-3.2.1.20 | α-glucosidase |