| Basic Information | |
|---|---|
| Species | Arabidopsis thaliana |
| Cazyme ID | AT1G69830.1 |
| Family | CBM45 |
| Protein Properties | Length: 888 Molecular Weight: 99842.1 Isoelectric Point: 5.9625 |
| Chromosome | Chromosome/Scaffold: 1 Start: 26288278 End: 26293053 |
| Description | alpha-amylase precursor, putative, expressed |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CBM45 | 305 | 381 | 6.6e-25 |
| VHWGVCKNGTKKWEIPSEPYPEETSLFKNKALRTRLQRKDDGNGSFGLFSLDGKLEGLCFVLKLNENTWLNYRGEDF | |||
| CBM45 | 126 | 209 | 3.8e-29 |
| LHWGVSYVGDTGSEWDQPPEDMRPPGSIAIKDYAIETPLKKLSEGDSFFEVAINLNLESSVAALNFVLKDEETGAWYQHKGRDF | |||
| GH13 | 517 | 806 | 2.4e-38 |
| QEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGGRLNWDDRAVVADDP HFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVD WINATSGAAGAFDVTTKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQGYAYILTHPGTPAVF | |||
| Full Sequence |
|---|
| Protein Sequence Length: 888 Download |
| MSTVPIESLL HHSYLRHNSK VNRGNRSFIP ISLNLRSHFT SNKLLHSIGK SVGVSSMNKS 60 PVAIRATSSD TAVVETAQSD DVIFKEIFPV QRIEKAEGKI YVRLKEVKEK NWELSVGCSI 120 PGKWILHWGV SYVGDTGSEW DQPPEDMRPP GSIAIKDYAI ETPLKKLSEG DSFFEVAINL 180 NLESSVAALN FVLKDEETGA WYQHKGRDFK VPLVDDVPDN GNLIGAKKGF GALGQLSNIP 240 LKQDKSSAET DSIEERKGLQ EFYEEMPISK RVADDNSVSV TARKCPETSK NIVSIETDLP 300 GDVTVHWGVC KNGTKKWEIP SEPYPEETSL FKNKALRTRL QRKDDGNGSF GLFSLDGKLE 360 GLCFVLKLNE NTWLNYRGED FYVPFLTSSS SPVETEAAQV SKPKRKTDKE VSASGFTKEI 420 ITEIRNLAID ISSHKNQKTN VKEVQENILQ EIEKLAAEAY SIFRSTTPAF SEEGVLEAEA 480 DKPDIKISSG TGSGFEILCQ GFNWESNKSG RWYLELQEKA DELASLGFTV LWLPPPTESV 540 SPEGYMPKDL YNLNSRYGTI DELKDTVKKF HKVGIKVLGD AVLNHRCAHF KNQNGVWNLF 600 GGRLNWDDRA VVADDPHFQG RGNKSSGDNF HAAPNIDHSQ DFVRKDIKEW LCWMMEEVGY 660 DGWRLDFVRG FWGGYVKDYM DASKPYFAVG EYWDSLSYTY GEMDYNQDAH RQRIVDWINA 720 TSGAAGAFDV TTKGILHTAL QKCEYWRLSD PKGKPPGVVG WWPSRAVTFI ENHDTGSTQG 780 HWRFPEGKEM QGYAYILTHP GTPAVFFDHI FSDYHSEIAA LLSLRNRQKL HCRSEVNIDK 840 SERDVYAAII DEKVAMKIGP GHYEPPNGSQ NWSVAVEGRD YKVWETS* 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PRK09441 | PRK09441 | 5.0e-48 | 496 | 827 | 413 | + cytoplasmic alpha-amylase; Reviewed | ||
| PLN00196 | PLN00196 | 7.0e-137 | 487 | 885 | 416 | + alpha-amylase; Provisional | ||
| cd11314 | AmyAc_arch_bac_plant_AmyA | 2.0e-163 | 497 | 836 | 343 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| PLN02361 | PLN02361 | 1.0e-170 | 493 | 885 | 399 | + alpha-amylase | ||
| PLN02784 | PLN02784 | 0 | 1 | 887 | 900 | + alpha-amylase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004556 | alpha-amylase activity |
| GO:0005509 | calcium ion binding |
| GO:0005975 | carbohydrate metabolic process |
| GO:0005983 | starch catabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAG52558.1 | 0 | 1 | 120 | 1 | 119 | AC010675_6 putative alpha-amylase; 60344-64829 [Arabidopsis thaliana] |
| GenBank | AAG52558.1 | 0.0008 | 53 | 214 | 171 | 325 | AC010675_6 putative alpha-amylase; 60344-64829 [Arabidopsis thaliana] |
| GenBank | AAG52558.1 | 0 | 156 | 887 | 95 | 826 | AC010675_6 putative alpha-amylase; 60344-64829 [Arabidopsis thaliana] |
| RefSeq | NP_564977.1 | 0 | 1 | 887 | 1 | 887 | AMY3 (ALPHA-AMYLASE-LIKE 3); alpha-amylase [Arabidopsis thaliana] |
| RefSeq | XP_002520134.1 | 0 | 1 | 887 | 1 | 900 | alpha-amylase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2qpu_C | 0 | 496 | 885 | 2 | 403 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
| PDB | 2qpu_B | 0 | 496 | 885 | 2 | 403 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
| PDB | 2qpu_A | 0 | 496 | 885 | 2 | 403 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
| PDB | 3bsg_A | 0 | 496 | 885 | 2 | 403 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
| PDB | 1rpk_A | 0 | 496 | 885 | 2 | 403 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-1823 | EC-3.2.1.1 | alpha-amylase |
| starch degradation I | RXN-1825 | EC-3.2.1.1 | alpha-amylase |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EG631183 | 912 | 1 | 888 | 0 |
| HO826981 | 407 | 482 | 888 | 0 |
| GR441435 | 293 | 440 | 732 | 0 |
| ES805448 | 328 | 453 | 780 | 0 |
| HO826981 | 27 | 451 | 477 | 1.6 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
