| Basic Information | |
|---|---|
| Species | Arabidopsis thaliana |
| Cazyme ID | AT1G75620.1 |
| Family | AA5 |
| Protein Properties | Length: 548 Molecular Weight: 60780.3 Isoelectric Point: 7.3516 |
| Chromosome | Chromosome/Scaffold: 1 Start: 28394779 End: 28396596 |
| Description | glyoxal oxidase-related, putative, expressed |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA5 | 34 | 546 | 0 |
| EGTWELLLPNVGISAMHSQLLHNDRVIMYDRTNFGPSNISLPNGACRSSPGDAVSKTDCTAHSVEYDVALNRIRPLTVQSNTWCSSGGVTPDGTLLQTGG DLDGERKVRLMDPCDDNSCDWIEVDNGLAARRWYATNHILPDGRQIIIGGRGQFNYEFFPKTNAPNFYSIPFLSETNDPGDENNLYPFVFLNTDGNLFIF ANNRAILLDYSTNTVVRTYPEIPGGDPRSYPSTGSAVLLPIKNLVLEVLVCGGAPKGSYNLSWRNTFVKALDTCARININDVNPQWIVEKMPRARVMGDM MLLPDGNVLLINGGSSGTAAWELGREPVLHPDLYHPDKPVGSRFEVQNPSTIPRMYHSIATLLRDGRILVGGSNPHAFYNFTGVLFPTELRLEAFSPSYL DTKYSSLRPSIVDPRPQTTVNYGRVLRLRFIVSGRVKSPVKVTMLFPSFTTHSFSMHQRLLVLDHVISFKLGISKIYEVRVRTPSSAILAPPGYYMVFVV NQDIPSEGLWVRL | |||
| Full Sequence |
|---|
| Protein Sequence Length: 548 Download |
| MAAKATTQIV SDVNHLRYFA VFFLLSCHVA SGDEGTWELL LPNVGISAMH SQLLHNDRVI 60 MYDRTNFGPS NISLPNGACR SSPGDAVSKT DCTAHSVEYD VALNRIRPLT VQSNTWCSSG 120 GVTPDGTLLQ TGGDLDGERK VRLMDPCDDN SCDWIEVDNG LAARRWYATN HILPDGRQII 180 IGGRGQFNYE FFPKTNAPNF YSIPFLSETN DPGDENNLYP FVFLNTDGNL FIFANNRAIL 240 LDYSTNTVVR TYPEIPGGDP RSYPSTGSAV LLPIKNLVLE VLVCGGAPKG SYNLSWRNTF 300 VKALDTCARI NINDVNPQWI VEKMPRARVM GDMMLLPDGN VLLINGGSSG TAAWELGREP 360 VLHPDLYHPD KPVGSRFEVQ NPSTIPRMYH SIATLLRDGR ILVGGSNPHA FYNFTGVLFP 420 TELRLEAFSP SYLDTKYSSL RPSIVDPRPQ TTVNYGRVLR LRFIVSGRVK SPVKVTMLFP 480 SFTTHSFSMH QRLLVLDHVI SFKLGISKIY EVRVRTPSSA ILAPPGYYMV FVVNQDIPSE 540 GLWVRLQ* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd02851 | E_set_GO_C | 4.0e-25 | 437 | 546 | 111 | + C-terminal Early set domain associated with the catalytic domain of galactose oxidase. E or "early" set domains are associated with the catalytic domain of galactose oxidase at the C-terminal end. Galactose oxidase is an extracellular monomeric enzyme which catalyzes the stereospecific oxidation of a broad range of primary alcohol substrates and possesses a unique mononuclear copper site essential for catalyzing a two-electron transfer reaction during the oxidation of primary alcohols to corresponding aldehydes. The second redox active center necessary for the reaction was found to be situated at a tyrosine residue. The C-terminal domain of galactose oxidase may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase, among others. | ||
| pfam09118 | DUF1929 | 4.0e-26 | 441 | 546 | 107 | + Domain of unknown function (DUF1929). Members of this family adopt a secondary structure consisting of a bundle of seven, mostly antiparallel, beta-strands surrounding a hydrophobic core. The 7 strands are arranged in 2 sheets, in a Greek-key topology. Their precise function, has not, as yet, been defined, though they are mostly found in sugar-utilising enzymes, such as galactose oxidase. | ||
| pfam07250 | Glyoxal_oxid_N | 1.0e-121 | 49 | 286 | 245 | + Glyoxal oxidase N-terminus. This family represents the N-terminus (approximately 300 residues) of a number of plant and fungal glyoxal oxidase enzymes. Glyoxal oxidase catalyzes the oxidation of aldehydes to carboxylic acids, coupled with reduction of dioxygen to hydrogen peroxide. It is an essential component of the extracellular lignin degradation pathways of the wood-rot fungus Phanerochaete chrysosporium. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003674 | molecular_function |
| GO:0008150 | biological_process |
| GO:0012505 | endomembrane system |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAG12543.1 | 0 | 49 | 547 | 1 | 504 | AC007797_3 Unknown Protein [Arabidopsis thaliana] |
| RefSeq | NP_173419.1 | 0 | 28 | 547 | 24 | 548 | glyoxal oxidase-related [Arabidopsis thaliana] |
| RefSeq | NP_177692.1 | 0 | 1 | 547 | 1 | 547 | glyoxal oxidase-related [Arabidopsis thaliana] |
| RefSeq | XP_002301997.1 | 0 | 35 | 546 | 5 | 523 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002510488.1 | 0 | 26 | 547 | 21 | 547 | Galactose oxidase precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2eid_A | 1e-17 | 107 | 546 | 217 | 637 | A Chain A, Galactose Oxidase W290g Mutant |
| PDB | 2eic_A | 5e-17 | 107 | 546 | 217 | 637 | A Chain A, Crystal Structure Of Galactose Oxidase Mutant W290f |
| PDB | 2wq8_A | 5e-17 | 107 | 546 | 239 | 659 | A Chain A, Glycan Labelling Using Engineered Variants Of Galactose Oxidase Obtained By Directed Evolution |
| PDB | 2eib_A | 8e-17 | 107 | 546 | 217 | 637 | A Chain A, Crystal Structure Of Galactose Oxidase, W290h Mutant |
| PDB | 1t2x_A | 1e-16 | 107 | 546 | 217 | 637 | A Chain A, Glactose Oxidase C383s Mutant Identified By Directed Evolution |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| DW517578 | 306 | 197 | 496 | 0 |
| EV120499 | 264 | 286 | 548 | 0 |
| CO101135 | 277 | 138 | 409 | 0 |
| ES816331 | 306 | 249 | 548 | 0 |
| CO118470 | 291 | 246 | 529 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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