| Basic Information | |
|---|---|
| Species | Arabidopsis thaliana |
| Cazyme ID | AT1G78580.1 |
| Family | GT20 |
| Protein Properties | Length: 943 Molecular Weight: 105976 Isoelectric Point: 7.1607 |
| Chromosome | Chromosome/Scaffold: 1 Start: 29552340 End: 29560912 |
| Description | trehalose-6-phosphate synthase, putative, expressed |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 90 | 558 | 0 |
| NRQRLLVVANRLPVSAVRRGEDSWSLEISAGGLVSALLGVKEFEARWIGWAGVNVPDEVGQKALSKALAEKRCIPVFLDEEIVHQYYNGYCNNILWPLFH YLGLPQEDRLATTRSFQSQFAAYKKANQMFADVVNEHYEEGDVVWCHDYHLMFLPKCLKEYNSKMKVGWFLHTPFPSSEIHRTLPSRSELLRSVLAADLV GFHTYDYARHFVSACTRILGLEGTPEGVEDQGRLTRVAAFPIGIDSDRFIRALEVPEVIQHMKELKERFAGRKVMLGVDRLDMIKGIPQKILAFEKFLEE NANWRDKVVLLQIAVPTRTDVPEYQKLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVACQEAKKGVLI LSEFAGAAQSLGAGAILVNPWNITEVAASIGQALNMTAEEREKRHRHNFHHVKTHTAQEWAETFVSELN | |||
| Full Sequence |
|---|
| Protein Sequence Length: 943 Download |
| MPGNKYNCSS SHIPLSRTER LLRDRELREK RKSNRARNPN DVAGSSENSE NDLRLEGDSS 60 RQYVEQYLEG AAAAMAHDDA CERQEVRPYN RQRLLVVANR LPVSAVRRGE DSWSLEISAG 120 GLVSALLGVK EFEARWIGWA GVNVPDEVGQ KALSKALAEK RCIPVFLDEE IVHQYYNGYC 180 NNILWPLFHY LGLPQEDRLA TTRSFQSQFA AYKKANQMFA DVVNEHYEEG DVVWCHDYHL 240 MFLPKCLKEY NSKMKVGWFL HTPFPSSEIH RTLPSRSELL RSVLAADLVG FHTYDYARHF 300 VSACTRILGL EGTPEGVEDQ GRLTRVAAFP IGIDSDRFIR ALEVPEVIQH MKELKERFAG 360 RKVMLGVDRL DMIKGIPQKI LAFEKFLEEN ANWRDKVVLL QIAVPTRTDV PEYQKLTSQV 420 HEIVGRINGR FGTLTAVPIH HLDRSLDFHA LCALYAVTDV ALVTSLRDGM NLVSYEFVAC 480 QEAKKGVLIL SEFAGAAQSL GAGAILVNPW NITEVAASIG QALNMTAEER EKRHRHNFHH 540 VKTHTAQEWA ETFVSELNDT VIEAQLRISK VPPELPQHDA IQRYSKSNNR LLILGFNATL 600 TEPVDNQGRR GDQIKEMDLN LHPELKGPLK ALCSDPSTTI VVLSGSSRSV LDKNFGEYDM 660 WLAAENGMFL RLTNGEWMTT MPEHLNMEWV DSVKHVFKYF TERTPRSHFE TRDTSLIWNY 720 KYADIEFGRL QARDLLQHLW TGPISNASVD VVQGSRSVEV RAVGVTKGAA IDRILGEIVH 780 SKSMTTPIDY VLCIGHFLGK DEDVYTFFEP ELPSDMPAIA RSRPSSDSGA KSSSGDRRPP 840 SKSTHNNNKS GSKSSSSSNS NNNNKSSQRS LQSERKSGSN HSLGNSRRPS PEKISWNVLD 900 LKGENYFSCA VGRTRTNARY LLGSPDDVVC FLEKLADTTS SP* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03063 | PLN03063 | 1.0e-12 | 871 | 941 | 73 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03064 | PLN03064 | 0 | 1 | 941 | 945 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03063 | PLN03063 | 0 | 91 | 814 | 724 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| TIGR02400 | trehalose_OtsA | 0 | 93 | 559 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| pfam00982 | Glyco_transf_20 | 0 | 92 | 559 | 474 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0003825 | alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity |
| GO:0005991 | trehalose metabolic process |
| GO:0005992 | trehalose biosynthetic process |
| GO:0009790 | embryo development |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAA69879.1 | 0 | 1 | 942 | 1 | 942 | trehalose-6-phosphate synthase [Arabidopsis thaliana] |
| RefSeq | NP_177979.1 | 0 | 1 | 942 | 1 | 942 | ATTPS1 (TREHALOSE-6-PHOSPHATE SYNTHASE); alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002285632.1 | 0 | 1 | 941 | 1 | 926 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002331300.1 | 0 | 1 | 940 | 1 | 922 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002531237.1 | 0 | 1 | 940 | 1 | 924 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1gz5_D | 0 | 93 | 561 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_C | 0 | 93 | 561 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_B | 0 | 93 | 561 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_A | 0 | 93 | 561 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 2wtx_D | 0 | 93 | 561 | 3 | 455 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| ES813375 | 361 | 454 | 814 | 0 |
| CX546763 | 302 | 81 | 382 | 0 |
| CV711307 | 294 | 90 | 383 | 0 |
| DW069910 | 288 | 179 | 466 | 0 |
| CX545499 | 305 | 396 | 700 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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