| Basic Information | |
|---|---|
| Species | Arabidopsis thaliana |
| Cazyme ID | AT2G06850.1 |
| Family | GH16 |
| Protein Properties | Length: 297 Molecular Weight: 34291.1 Isoelectric Point: 9.1999 |
| Chromosome | Chromosome/Scaffold: 2 Start: 2763526 End: 2765720 |
| Description | glycosyl hydrolases family 16, putative, expressed |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH16 | 52 | 213 | 1.5e-27 |
| NGGSELQLILDKYTGTGFQSKGSYLFGHFSMHIKLPAGDTAGVVTAFYLSSTNNEHDEIDFEFLGNRTGQPAILQTNVFTGGKGNREQRIYLWFDPSKAY HTYSILWNMYQIVFFVDNIPIRTFKNAKDLGVRFPFNQPMKLYSSLWNADDWATRGGLEKTN | |||
| Full Sequence |
|---|
| Protein Sequence Length: 297 Download |
| MTVSSSPWAL MALFLMVSST MVMAIPPRKA IDVPFGRNYV PTWAFDHQKQ FNGGSELQLI 60 LDKYTGTGFQ SKGSYLFGHF SMHIKLPAGD TAGVVTAFYL SSTNNEHDEI DFEFLGNRTG 120 QPAILQTNVF TGGKGNREQR IYLWFDPSKA YHTYSILWNM YQIVFFVDNI PIRTFKNAKD 180 LGVRFPFNQP MKLYSSLWNA DDWATRGGLE KTNWANAPFV ASYKGFHIDG CQASVEAKYC 240 ATQGRMWWDQ KEFRDLDAEQ WRRLKWVRMK WTIYNYCTDR TRFPVMPAEC KRDRDA* 300 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd02175 | GH16_lichenase | 1.0e-25 | 36 | 204 | 180 | + lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure. | ||
| cd02183 | GH16_fungal_CRH1_transglycosylase | 5.0e-26 | 52 | 224 | 188 | + glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
| pfam00722 | Glyco_hydro_16 | 3.0e-75 | 35 | 215 | 183 | + Glycosyl hydrolases family 16. | ||
| PLN03161 | PLN03161 | 6.0e-97 | 15 | 290 | 280 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional | ||
| cd02176 | GH16_XET | 2.0e-158 | 30 | 290 | 263 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005576 | extracellular region |
| GO:0005618 | cell wall |
| GO:0005737 | cytoplasm |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACD03215.1 | 0 | 11 | 296 | 10 | 293 | xyloglucan endotransglucosylase/hydrolase 5 [Actinidia deliciosa] |
| GenBank | ACD03216.1 | 0 | 3 | 296 | 1 | 293 | xyloglucan endotransglucosylase/hydrolase 6 [Actinidia deliciosa] |
| RefSeq | NP_178708.1 | 0 | 1 | 296 | 1 | 296 | EXGT-A1 (ENDOXYLOGLUCAN TRANSFERASE); hydrolase, acting on glycosyl bonds / xyloglucan:xyloglucosyl transferase [Arabidopsis thaliana] |
| RefSeq | NP_196891.1 | 0 | 9 | 295 | 6 | 292 | EXGT-A4 (ENDOXYLOGLUCAN TRANSFERASE A4); hydrolase, acting on glycosyl bonds / hydrolase, hydrolyzing O-glycosyl compounds / xyloglucan:xyloglucosyl transferase [Arabidopsis thaliana] |
| Swiss-Prot | Q6YDN9 | 0 | 1 | 296 | 1 | 295 | XTH_BRAOB RecName: Full=Xyloglucan endotransglucosylase/hydrolase; AltName: Full=BobXET16A; Flags: Precursor |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1un1_B | 0 | 28 | 295 | 10 | 277 | A Chain A, Crystal Structure Of The Polygalacturonase From Colletotrichum Lupini And Its Implications For The Interaction With Polygalacturonase- Inhibiting Proteins |
| PDB | 1un1_A | 0 | 28 | 295 | 10 | 277 | A Chain A, Crystal Structure Of The Polygalacturonase From Colletotrichum Lupini And Its Implications For The Interaction With Polygalacturonase- Inhibiting Proteins |
| PDB | 1umz_B | 0 | 28 | 295 | 10 | 277 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 1umz_A | 0 | 28 | 295 | 10 | 277 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 2vh9_B | 0 | 14 | 290 | 15 | 290 | A Chain A, Crystal Structure Of Nxg1-Deltayniig In Complex With Xllg, A Xyloglucan Derived Oligosaccharide |
