| Basic Information | |
|---|---|
| Species | Arabidopsis thaliana |
| Cazyme ID | AT2G36190.1 |
| Family | GH32 |
| Protein Properties | Length: 592 Molecular Weight: 67412.7 Isoelectric Point: 8.0865 |
| Chromosome | Chromosome/Scaffold: 2 Start: 15174730 End: 15177870 |
| Description | glycosyl hydrolases, putative, expressed |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
 | |||
| Family | Start | End | Evalue |
| GH32 | 54 | 372 | 0 |
| HFQPPKHWINDPNGPVYYKGLYHLFYQYNTKGAVWGNIIWAHSVSKDLVNWEALEPALSPSKWFDIGGTWSGSITIVPGKGPIILYTGVNQNETQLQNYA IPEDPSDPYLRKWIKPDDNPIAIPDYTMNGSAFRDPTTAWFSKDGHWRTVVGSKRKRRGIAYIYRSRDFKHWVKAKHPVHSKQSTGMWECPDFFPVSLTD FRNGLDLDYVGPNTKHVLKVSLDITRYEYYTLGKYDLKKDRYIPDGNTPDGWEGLRFDYGNFYASKTFFDYKKNRRILWGWANESDTVEDDILKGWAGLQ VIPRTVLLDSSKKQLVFWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 592 Download |
| MAISNVISVL LLLLVLINLS NQNIKGIDAF HQIYEELQSE SVESVNHLHR PSFHFQPPKH 60 WINDPNGPVY YKGLYHLFYQ YNTKGAVWGN IIWAHSVSKD LVNWEALEPA LSPSKWFDIG 120 GTWSGSITIV PGKGPIILYT GVNQNETQLQ NYAIPEDPSD PYLRKWIKPD DNPIAIPDYT 180 MNGSAFRDPT TAWFSKDGHW RTVVGSKRKR RGIAYIYRSR DFKHWVKAKH PVHSKQSTGM 240 WECPDFFPVS LTDFRNGLDL DYVGPNTKHV LKVSLDITRY EYYTLGKYDL KKDRYIPDGN 300 TPDGWEGLRF DYGNFYASKT FFDYKKNRRI LWGWANESDT VEDDILKGWA GLQVIPRTVL 360 LDSSKKQLVF WPVEEIESLR GNYVRMNNHD IKMGQRIEVK GITPAQADVE VTFYVGSLEK 420 AEIFDPSFTW KPLELCNIKG SNVRGGVGPF GLITLATPDL EEYTPVFFRV FNDTKTHKPK 480 VLMCSDARPS SLKQDTGLLA KDRMYKPSFA GFVDVDMADG RISLRSLIDH SVVESFGALG 540 KTVITSRVYP VKAVKENAHL YVFNNGTQTV TIESLNAWNM DRPLQMNDGA L* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
 | ||||||||
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 2.0e-50 | 49 | 382 | 358 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 1.0e-62 | 31 | 550 | 538 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 3.0e-87 | 60 | 375 | 328 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 2.0e-144 | 54 | 372 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 5.0e-174 | 54 | 540 | 496 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0004575 | sucrose alpha-glucosidase activity |
| GO:0005618 | cell wall |
| GO:0005982 | starch metabolic process |
| GO:0010131 | sucrose catabolic process, using invertase or sucrose synthase |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAM61359.1 | 0 | 1 | 591 | 1 | 591 | putative beta-fructofuranosidase (invertase) [Arabidopsis thaliana] |
| GenBank | ABK28599.1 | 0 | 10 | 590 | 9 | 589 | unknown [Arabidopsis thaliana] |
| DDBJ | BAC42957.1 | 0 | 10 | 590 | 9 | 589 | putative beta-fructofuranosidase [Arabidopsis thaliana] |
| RefSeq | NP_190828.2 | 0 | 10 | 590 | 9 | 589 | AtcwINV2 (Arabidopsis thaliana cell wall invertase 2); hydrolase, hydrolyzing O-glycosyl compounds |
| RefSeq | NP_565837.1 | 0 | 1 | 591 | 1 | 591 | AtcwINV4 (Arabidopsis thaliana cell wall invertase 4); hydrolase, hydrolyzing O-glycosyl compounds |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2ac1_A | 0 | 44 | 581 | 3 | 536 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
| PDB | 2xqr_K | 0 | 46 | 581 | 1 | 532 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 46 | 581 | 1 | 532 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 46 | 581 | 1 | 532 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_E | 0 | 46 | 581 | 1 | 532 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
