| Basic Information | |
|---|---|
| Species | Arabidopsis thaliana |
| Cazyme ID | AT2G36390.1 |
| Family | GH13 |
| Protein Properties | Length: 859 Molecular Weight: 97659.8 Isoelectric Point: 5.3124 |
| Chromosome | Chromosome/Scaffold: 2 Start: 15264237 End: 15270092 |
| Description | 1,4-alpha-glucan-branching enzyme, chloroplast precursor, putative, expressed |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH13 | 353 | 674 | 4.5e-29 |
| LPRIKKLGYNAVQIMAIQEHAYYASFGYHVTNFFAPSSRFGTPDDLKSLIDKAHELGLVVLMDIVHSHASKNTLDGLDMFDGTDGQYFHSGSRGYHWMWD SRLFNYGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLQVEFTGNYNEYFGYSTDVDAVVYLMLVNDLIHGLYPEAIVVGEDVSGMPAFCVP VEDGGVGFDYRLHMAVADKWIELLKKRDEDWQVGDITFTLTNRRWGEKCVVYAESHDQALVGDKTIAFWLMDKDMYDFMAVDRQATPRVDRGIALHKMIR LITMGLGGEGYLNFMGNEFGHP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 859 Download |
| MVYTISGVRF PHLPSIKKKN SSLHSFNEDL RRSNAVSFSL RKDSRSSGKV FARKPSYDSD 60 SSSLATTASE KLRGHQSDSS SSASDQVQSR DTVSDDTQVL GNVDVQKTEE AQETETLDQT 120 SALSTSGSIS YKEDFAKMSH SVDQEVGQRK IPPPGDGKRI YDIDPMLNSH RNHLDYRYGQ 180 YRKLREEIDK NEGGLEAFSR GYEIFGFTRS ATGITYREWA PGAKAASLIG DFNNWNAKSD 240 VMARNDFGVW EIFLPNNADG SPAIPHGSRV KIRMDTPSGI KDSIPAWIKY SVQPPGEIPY 300 NGVYYDPPEE DKYAFKHPRP KKPTSLRIYE SHVGMSSTEP KINTYANFRD DVLPRIKKLG 360 YNAVQIMAIQ EHAYYASFGY HVTNFFAPSS RFGTPDDLKS LIDKAHELGL VVLMDIVHSH 420 ASKNTLDGLD MFDGTDGQYF HSGSRGYHWM WDSRLFNYGS WEVLRYLLSN ARWWLEEYKF 480 DGFRFDGVTS MMYTHHGLQV EFTGNYNEYF GYSTDVDAVV YLMLVNDLIH GLYPEAIVVG 540 EDVSGMPAFC VPVEDGGVGF DYRLHMAVAD KWIELLKKRD EDWQVGDITF TLTNRRWGEK 600 CVVYAESHDQ ALVGDKTIAF WLMDKDMYDF MAVDRQATPR VDRGIALHKM IRLITMGLGG 660 EGYLNFMGNE FGHPEWIDFP RTDQHLPDGR VIAGNNGSYD KCRRRFDLGD AEYLRYHGLQ 720 EFDRAMQNLE ETYGFMTSEH QYISRKDEGD RVIVFERGNL LFVFNFHWTN SYSDYRIGCS 780 VPGKYKIVLD SDNSLFGGFN RLDDSAEFFT SDGRHDDRPC SFMVYAPCRT AVVYAAVDDD 840 DDDERSSLVP IGLLPEDV* 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN02960 | PLN02960 | 2.0e-10 | 177 | 254 | 84 | + alpha-amylase | ||
| PLN03244 | PLN03244 | 6.0e-137 | 262 | 830 | 578 | + alpha-amylase; Provisional | ||
| PLN02447 | PLN02447 | 0 | 150 | 830 | 685 | + 1,4-alpha-glucan-branching enzyme | ||
| cd11321 | AmyAc_bac_euk_BE | 0 | 309 | 724 | 417 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| PLN02960 | PLN02960 | 0 | 262 | 830 | 574 | + alpha-amylase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0003844 | 1,4-alpha-glucan branching enzyme activity |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| GO:0005982 | starch metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAB03099.1 | 0 | 8 | 858 | 4 | 854 | starch branching enzyme class II [Arabidopsis thaliana] |
| GenBank | ABO31358.1 | 0 | 1 | 838 | 1 | 840 | starch branching enzyme II-1 [Malus x domestica] |
| GenBank | ABO31359.1 | 0 | 1 | 838 | 1 | 840 | starch branching enzyme II-2 [Malus x domestica] |
| EMBL | CAA56319.1 | 0 | 1 | 838 | 1 | 846 | starch branching enzyme I [Pisum sativum] |
| RefSeq | NP_181180.1 | 0 | 1 | 858 | 1 | 858 | SBE2.1 (starch branching enzyme 2.1); 1,4-alpha-glucan branching enzyme [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3amk_A | 0 | 160 | 845 | 13 | 701 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 3vu2_B | 0 | 160 | 845 | 13 | 701 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 3vu2_A | 0 | 160 | 845 | 13 | 701 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 3aml_A | 0 | 160 | 845 | 13 | 701 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 1m7x_D | 0 | 200 | 800 | 9 | 579 | A Chain A, The X-Ray Crystallographic Structure Of Branching Enzyme |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch biosynthesis | RXN-7710 | EC-2.4.1.18 | 1,4-alpha-glucan branching enzyme |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO794536 | 677 | 154 | 830 | 0 |
| HO777638 | 621 | 210 | 830 | 0 |
| HO458123 | 388 | 443 | 830 | 0 |
| HO458123 | 296 | 154 | 442 | 0 |
| HO777638 | 47 | 163 | 209 | 0.0000000002 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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