| Basic Information | |
|---|---|
| Species | Arabidopsis thaliana |
| Cazyme ID | AT3G18660.1 |
| Family | GT8 |
| Protein Properties | Length: 656 Molecular Weight: 75643.1 Isoelectric Point: 7.8968 |
| Chromosome | Chromosome/Scaffold: 3 Start: 6419060 End: 6421983 |
| Description | glycosyl transferase, putative, expressed |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT8 | 322 | 530 | 2.09999e-41 |
| TILHSAHVYVCGAIAAAQSIRQSGSTRDLVILVDDNISGYHRSGLEAAGWQIRTIQRIRNPKAEKDAYNEWNYSKFRLWQLTDYDKIIFIDADLLILRNI DFLFSMPEISATGNNGTLFNSGVMVIEPCNCTFQLLMEHINEIESYNGGDQGYLNEVFTWWHRIPKHMNFLKHFWIGDEDDAKRKKTELFGAEPPVLYVL HYLGMKPWL | |||
| Full Sequence |
|---|
| Protein Sequence Length: 656 Download |
| MANSPAAPAP TTTTGGDSRR RLSASIEAIC KRRFRRNSKG GGRSDMVKPF NIINFSTQDK 60 NSSCCCFTKF QIVKLLLFIL LSATLFTIIY SPEAYHHSLS HSSSRRQDPR YFSDLDINWD 120 DVTKTLENIE EGRTIGVLNF DSNEIQRWRE VSKSKDNGDE EKVVVLNLDY ADKNVTWDAL 180 YPEWIDEEQE TEVPVCPNIP NIKVPTRRLD LIVVKLPCRK EGNWSRDVGR LHLQLAAATV 240 AASAKGFFRG HVFFVSRCFP IPNLFRCKDL VSRRGDVWLY KPNLDTLRDK LQLPVGSCEL 300 SLPLGIQDRP SLGNPKREAY ATILHSAHVY VCGAIAAAQS IRQSGSTRDL VILVDDNISG 360 YHRSGLEAAG WQIRTIQRIR NPKAEKDAYN EWNYSKFRLW QLTDYDKIIF IDADLLILRN 420 IDFLFSMPEI SATGNNGTLF NSGVMVIEPC NCTFQLLMEH INEIESYNGG DQGYLNEVFT 480 WWHRIPKHMN FLKHFWIGDE DDAKRKKTEL FGAEPPVLYV LHYLGMKPWL CYRDYDCNFN 540 SDIFVEFATD IAHRKWWMVH DAMPQELHQF CYLRSKQKAQ LEYDRRQAEA ANYADGHWKI 600 RVKDPRFKIC IDKLCNWKSM LRHWGESNWT DYESFVPTPP AITVDRRSSL PGHNL* 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00505 | Glyco_transf_8 | 8.0e-13 | 323 | 529 | 245 | + Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+. | ||
| cd06914 | GT8_GNT1 | 2.0e-15 | 330 | 480 | 164 | + GNT1 is a fungal enzyme that belongs to the GT 8 family. N-acetylglucosaminyltransferase is a fungal enzyme that catalyzes the addition of N-acetyl-D-glucosamine to mannotetraose side chains by an alpha 1-2 linkage during the synthesis of mannan. The N-acetyl-D-glucosamine moiety in mannan plays a role in the attachment of mannan to asparagine residues in proteins. The mannotetraose and its N-acetyl-D-glucosamine derivative side chains of mannan are the principle immunochemical determinants on the cell surface. N-acetylglucosaminyltransferase is a member of glycosyltransferase family 8, which are, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed, retaining glycosyltransferases. | ||
| PLN00176 | PLN00176 | 6.0e-16 | 299 | 428 | 133 | + galactinol synthase | ||
| pfam01501 | Glyco_transf_8 | 2.0e-43 | 321 | 532 | 256 | + Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase. | ||
| cd02537 | GT8_Glycogenin | 1.0e-71 | 318 | 563 | 256 | + Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen. Glycogenin initiates the biosynthesis of glycogen by incorporating glucose residues through a self-glucosylation reaction at a Tyr residue, and then acts as substrate for chain elongation by glycogen synthase and branching enzyme. It contains a conserved DxD motif and an N-terminal beta-alpha-beta Rossmann-like fold that are common to the nucleotide-binding domains of most glycosyltransferases. The DxD motif is essential for coordination of the catalytic divalent cation, most commonly Mn2+. Glycogenin can be classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. It is placed in glycosyltransferase family 8 which includes lipopolysaccharide glucose and galactose transferases and galactinol synthases. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0005982 | starch metabolic process |
| GO:0009058 | biosynthetic process |
| GO:0009507 | chloroplast |
| GO:0016757 | transferase activity, transferring glycosyl groups |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | NP_001030722.1 | 0 | 1 | 655 | 1 | 659 | PGSIP1 (PLANT GLYCOGENIN-LIKE STARCH INITIATION PROTEIN 1); transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | NP_566615.1 | 0 | 1 | 655 | 1 | 655 | PGSIP1 (PLANT GLYCOGENIN-LIKE STARCH INITIATION PROTEIN 1); transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002275240.1 | 0 | 11 | 652 | 3 | 636 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002310513.1 | 0 | 17 | 652 | 9 | 629 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002517237.1 | 0 | 12 | 652 | 8 | 629 | glycogenin, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3u2w_B | 8e-22 | 318 | 529 | 5 | 221 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1), Apo Form |
| PDB | 3u2w_A | 8e-22 | 318 | 529 | 5 | 221 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1), Apo Form |
| PDB | 3qvb_A | 8e-22 | 318 | 529 | 5 | 221 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1), Apo Form |
| PDB | 3q4s_A | 8e-22 | 318 | 529 | 5 | 221 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1), Apo Form |
| PDB | 3u2x_B | 8e-22 | 318 | 529 | 5 | 221 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1), Apo Form |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| FG552677 | 319 | 222 | 538 | 0 |
| FG568830 | 242 | 377 | 618 | 0 |
| DY825283 | 302 | 336 | 635 | 0 |
| DV711190 | 267 | 348 | 614 | 0 |
| DV711190 | 44 | 599 | 642 | 0.0005 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
