| Basic Information | |
|---|---|
| Species | Arabidopsis thaliana |
| Cazyme ID | AT3G29320.1 |
| Family | GT35 |
| Protein Properties | Length: 963 Molecular Weight: 108585 Isoelectric Point: 5.1277 |
| Chromosome | Chromosome/Scaffold: 3 Start: 11252807 End: 11257814 |
| Description | alpha-glucan phosphorylast isozyme, putative, expressed |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT35 | 180 | 957 | 0 |
| ALKRLGFDLESVASQEPDPALGNGGLGRLASCFLDSMATLNYPAWGYGLRYKYGLFKQRITKDGQEEAAEDWLELSNPWEIVRNDVSYPIKFYGKVVFGS DGKKRWIGGEDIVAVAYDVPIPGYKTKTTINLRLWSTKAPSEDFDLSSYNSGKHTEAAEALFNAEKICFVLYPGDESTEGKALRLKQQYTLCSASLQDIV ARFETRSGGNVNWEEFPEKVAVQMNDTHPTLCIPELMRILMDLKGLSWEDAWKITQRTVAYTNHTVLPEALEKWSLELMEKLLPRHVEIIEKIDEELVRT IVSEYGTADPDLLEEKLKAMRILENVELPSAFADVIVKPVNKPVTAKDAQNGVKTEQEEEKTAGEEEEDEVIPEPTVEPPKMVRMANLAVVGGHAVNGVA EIHSEIVKQDVFNDFVQLWPEKFQNKTNGVTPRRWIRFCNPYLSDIITNWIGTEDWVLNTEKVAELRKFADNEDLQSEWRAAKKKNKLKVVSLIKERTGY TVSPDAMFDIQIKRIHEYKRQLLNILGIVYRYKKMKEMSASEREKAFVPRVCIFGGKAFATYVQAKRIVKFITDVASTINHDPEIGDLLKVIFVPDYNVS VAELLIPASELSQHISTAGMEASGTSNMKFSMNGCVLIGTLDGANVEIREEVGEENFFLFGAKADQIVNLRKERAEGKFVPDPTFEEVKKFVGSGVFGSN SYDELIGSLEGNEGFGRADYFLVGKDFPSYIECQEKVDEAYRDQKRWTRMSIMNTAGSFKFSSDRTIHEYAKDIWNIK | |||
| Full Sequence |
|---|
| Protein Sequence Length: 963 Download |
| MDTMRISGVS TGAEVLIQCN SLSSLVSRRC DDGKWRTRMF PARNRDLRPS PTRRSFLSVK 60 SISSEPKAKV TDAVLDSEQE VFISSMNPFA PDAASVASSI KYHAEFTPLF SPEKFELPKA 120 FFATAQSVRD ALIMNWNATY EYYNRVNVKQ AYYLSMEFLQ GRALSNAVGN LGLNSAYGDA 180 LKRLGFDLES VASQEPDPAL GNGGLGRLAS CFLDSMATLN YPAWGYGLRY KYGLFKQRIT 240 KDGQEEAAED WLELSNPWEI VRNDVSYPIK FYGKVVFGSD GKKRWIGGED IVAVAYDVPI 300 PGYKTKTTIN LRLWSTKAPS EDFDLSSYNS GKHTEAAEAL FNAEKICFVL YPGDESTEGK 360 ALRLKQQYTL CSASLQDIVA RFETRSGGNV NWEEFPEKVA VQMNDTHPTL CIPELMRILM 420 DLKGLSWEDA WKITQRTVAY TNHTVLPEAL EKWSLELMEK LLPRHVEIIE KIDEELVRTI 480 VSEYGTADPD LLEEKLKAMR ILENVELPSA FADVIVKPVN KPVTAKDAQN GVKTEQEEEK 540 TAGEEEEDEV IPEPTVEPPK MVRMANLAVV GGHAVNGVAE IHSEIVKQDV FNDFVQLWPE 600 KFQNKTNGVT PRRWIRFCNP YLSDIITNWI GTEDWVLNTE KVAELRKFAD NEDLQSEWRA 660 AKKKNKLKVV SLIKERTGYT VSPDAMFDIQ IKRIHEYKRQ LLNILGIVYR YKKMKEMSAS 720 EREKAFVPRV CIFGGKAFAT YVQAKRIVKF ITDVASTINH DPEIGDLLKV IFVPDYNVSV 780 AELLIPASEL SQHISTAGME ASGTSNMKFS MNGCVLIGTL DGANVEIREE VGEENFFLFG 840 AKADQIVNLR KERAEGKFVP DPTFEEVKKF VGSGVFGSNS YDELIGSLEG NEGFGRADYF 900 LVGKDFPSYI ECQEKVDEAY RDQKRWTRMS IMNTAGSFKF SSDRTIHEYA KDIWNIKQVE 960 LP* 1020 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 0 | 97 | 956 | 869 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| pfam00343 | Phosphorylase | 0 | 180 | 957 | 783 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| PRK14986 | PRK14986 | 0 | 123 | 961 | 845 | + glycogen phosphorylase; Provisional | ||
| PRK14985 | PRK14985 | 0 | 153 | 957 | 820 | + maltodextrin phosphorylase; Provisional | ||
| COG0058 | GlgP | 0 | 91 | 957 | 872 | + Glucan phosphorylase [Carbohydrate transport and metabolism] | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004645 | phosphorylase activity |
| GO:0005975 | carbohydrate metabolic process |
| GO:0009266 | response to temperature stimulus |
| GO:0009414 | response to water deprivation |
| GO:0009507 | chloroplast |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACJ11757.1 | 0 | 57 | 962 | 4 | 935 | alpha-1,4 glucan phosphorylase [Gossypium hirsutum] |
| RefSeq | NP_189578.1 | 0 | 1 | 962 | 1 | 962 | glucan phosphorylase, putative [Arabidopsis thaliana] |
| RefSeq | XP_002279075.1 | 0 | 36 | 962 | 36 | 958 | PREDICTED: similar to Alpha-1,4 glucan phosphorylase L-1 isozyme, chloroplastic/amyloplastic [Vitis vinifera] |
| RefSeq | XP_002305367.1 | 0 | 53 | 962 | 9 | 949 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002526085.1 | 0 | 18 | 962 | 16 | 977 | glycogen phosphorylase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1z8d_A | 0 | 103 | 957 | 35 | 829 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| PDB | 3cem_B | 0 | 92 | 957 | 1 | 806 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| PDB | 3cem_A | 0 | 92 | 957 | 1 | 806 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| PDB | 3cej_B | 0 | 92 | 957 | 1 | 806 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| PDB | 3cej_A | 0 | 92 | 957 | 1 | 806 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-1826 | EC-2.4.1.1 | phosphorylase |
| Hydropathy |
|---|
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO778303 | 864 | 120 | 963 | 0 |
| HO797178 | 384 | 580 | 963 | 0 |
| HO613954 | 507 | 457 | 963 | 0 |
| HO418036 | 580 | 306 | 882 | 0 |
| HO778303 | 123 | 3 | 122 | 1e-16 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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