| Basic Information | |
|---|---|
| Species | Arabidopsis thaliana |
| Cazyme ID | AT4G20820.1 |
| Family | AA7 |
| Protein Properties | Length: 533 Molecular Weight: 60392.3 Isoelectric Point: 9.1384 |
| Chromosome | Chromosome/Scaffold: 4 Start: 11150160 End: 11151758 |
| Description | reticuline oxidase-like protein precursor, putative, expressed |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 77 | 523 | 0 |
| SAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPA GVCPTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIH RWQFVADKVSDDLFIRVMLQRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAELGEEPINVLTKRTRASLAFKAKSDFVQ EPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYWRGDVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLG MYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSI | |||
| Full Sequence |
|---|
| Protein Sequence Length: 533 Download |
| MKFQSFFSSV LIFFTTSTLL LSIPHPVSAN RSNQSSFLQC LSLQLNDSNI VSKVIHTPND 60 TSFSSVLASS IQNQRFSAPD VPKPVLILTP VQPSDVQSAV KCARRFGIHI RTRSGGHDYE 120 GLSYVTHKPF VILDLRNLRS ITVDVDNRSV WVQTGATIGE LYYEIGKKNR TLAFPAGVCP 180 TVGVGGHFSG GGYGTLLRKH GLAADHVIDA RVVDARGRIL ERREMGEDFF WAIRGGGGSS 240 FCVVLSWKIG LINVPSTVTV FNVTKFSEQS ALKIIHRWQF VADKVSDDLF IRVMLQRYKN 300 MVRASFPGLY LGSVKNLLKM VNKEFPELGL EEDDCTEMSW IESVIWFAEL GEEPINVLTK 360 RTRASLAFKA KSDFVQEPMP KTAISKLWRR LQEPEAEHAQ LIFTPFGGKM SEIADYETPF 420 PHRKGNIYEI QYLNYWRGDV KEKYMRWVER VYDDMSEFVA KSPRGAYINL RDLDLGMYVG 480 VKRSKYEEGK SWGVKYFKNN FERLVRVKTS VDPSDFFCDE QSIPPFTFVE VI* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam08031 | BBE | 1.0e-14 | 466 | 524 | 59 | + Berberine and berberine like. This domain is found in the berberine bridge and berberine bridge- like enzymes which are involved in the biosynthesis of numerous isoquinoline alkaloids. They catalyze the transformation of the N-methyl group of (S)-reticuline into the C-8 berberine bridge carbon of (S)-scoulerine. | ||
| COG0277 | GlcD | 4.0e-17 | 84 | 526 | 460 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam01565 | FAD_binding_4 | 7.0e-26 | 84 | 221 | 139 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009055 | electron carrier activity |
| GO:0016491 | oxidoreductase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAO29955.1 | 0 | 45 | 524 | 1 | 489 | berberine bridge enzyme-like protein [Arabidopsis thaliana] |
| DDBJ | BAE99575.1 | 0 | 27 | 532 | 5 | 513 | berberine bridge enzyme-like protein [Arabidopsis thaliana] |
| RefSeq | NP_193814.1 | 0 | 1 | 532 | 1 | 532 | FAD-binding domain-containing protein [Arabidopsis thaliana] |
| RefSeq | NP_199254.1 | 0 | 1 | 532 | 1 | 535 | FAD-binding domain-containing protein [Arabidopsis thaliana] |
| RefSeq | NP_199257.1 | 0 | 1 | 524 | 1 | 527 | FAD-binding domain-containing protein [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3vte_A | 0 | 34 | 526 | 4 | 513 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| PDB | 4dns_B | 0 | 26 | 526 | 2 | 496 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 4dns_A | 0 | 26 | 526 | 2 | 496 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 3tsj_B | 0 | 34 | 526 | 8 | 496 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 3tsj_A | 0 | 34 | 526 | 8 | 496 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cannabinoid biosynthesis | RXN-7854 | EC-1.21.3 | tetrahydrocannabinolic acid synthase |
