| Basic Information | |
|---|---|
| Species | Arabidopsis thaliana |
| Cazyme ID | AT4G20830.2 |
| Family | AA7 |
| Protein Properties | Length: 541 Molecular Weight: 60287.2 Isoelectric Point: 10.0367 |
| Chromosome | Chromosome/Scaffold: 4 Start: 11155460 End: 11157322 |
| Description | reticuline oxidase-like protein precursor, putative, expressed |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 76 | 533 | 0 |
| NARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISDKPFFILDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVH GFPAGVCPTVGVGGHLSGGGYGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKLVPVPSVVTVFRVEQYMDSGAV DMVHKWQSVGPKTDPNLFMRMLIQPVTRKKVKTVRASVVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRNLDT SSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHRNKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPR SSYFNYRDVDIGVNDHGANSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSI | |||
| Full Sequence |
|---|
| Protein Sequence Length: 541 Download |
| MLTTPPRTFV SVPFFFFFLL FLSLPLSSFS QSNSVYNSFL KCFSDKTKSP QSQITDNVFS 60 QTNPAFSSVL RAYIRNARFN TSSTLKPTII ITPRSESHVS AAVTCSKTLN FLLKIRSGGH 120 DYDGLSYISD KPFFILDMSN IRDVSVDIAS NSAWISAGAT LGEVYYRIWE KSRVHGFPAG 180 VCPTVGVGGH LSGGGYGNMV RKFGLSVDYV EDAKIVDVNG RVLDRKAMGE DLFWAITGGG 240 GGSYGVVLGY KVKLVPVPSV VTVFRVEQYM DSGAVDMVHK WQSVGPKTDP NLFMRMLIQP 300 VTRKKVKTVR ASVVALFLGR ADEVVALLSK EFPELGLKKE NCSEMTWFQS ALWWDNRLNA 360 TQVDPKVFLD RNLDTSSFGK RKSDYVATAI PKKGIESLFK KMIELGKIGL VFNPYGGKMA 420 EVAVNAKPFP HRNKLFKIQY SVNWKENSAE IEKGYLNQAK VLYSFMTGFV SKNPRSSYFN 480 YRDVDIGVND HGANSYKEGE VYGRKYFGEN FDRLVKIKTA VDPGNFFRNE QSIPTLKSKA 540 * 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG0277 | GlcD | 3.0e-7 | 68 | 235 | 179 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam08031 | BBE | 3.0e-17 | 477 | 534 | 58 | + Berberine and berberine like. This domain is found in the berberine bridge and berberine bridge- like enzymes which are involved in the biosynthesis of numerous isoquinoline alkaloids. They catalyze the transformation of the N-methyl group of (S)-reticuline into the C-8 berberine bridge carbon of (S)-scoulerine. | ||
| pfam01565 | FAD_binding_4 | 3.0e-19 | 87 | 224 | 139 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005739 | mitochondrion |
| GO:0005773 | vacuole |
| GO:0005886 | plasma membrane |
| GO:0006979 | response to oxidative stress |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAD94511.1 | 0 | 32 | 539 | 16 | 528 | hypothetical protein [Arabidopsis thaliana] |
| RefSeq | NP_172642.3 | 0 | 15 | 539 | 8 | 532 | FAD binding / catalytic/ electron carrier/ oxidoreductase [Arabidopsis thaliana] |
| RefSeq | NP_193815.2 | 0 | 1 | 539 | 1 | 539 | FAD-binding domain-containing protein [Arabidopsis thaliana] |
| RefSeq | NP_193816.1 | 0 | 35 | 540 | 34 | 539 | FAD-binding domain-containing protein [Arabidopsis thaliana] |
| RefSeq | NP_974580.1 | 0 | 1 | 540 | 1 | 540 | FAD-binding domain-containing protein [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 4dns_B | 0 | 32 | 536 | 6 | 496 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 4dns_A | 0 | 32 | 536 | 6 | 496 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 3vte_A | 0 | 38 | 536 | 6 | 513 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| PDB | 3tsj_B | 0 | 58 | 539 | 24 | 499 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| PDB | 3tsj_A | 0 | 58 | 539 | 24 | 499 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cannabinoid biosynthesis | RXN-7854 | EC-1.21.3 | tetrahydrocannabinolic acid synthase |
