| Basic Information | |
|---|---|
| Species | Arabidopsis thaliana |
| Cazyme ID | AT5G03650.1 |
| Family | GH13 |
| Protein Properties | Length: 806 Molecular Weight: 92591.4 Isoelectric Point: 5.6793 |
| Chromosome | Chromosome/Scaffold: 5 Start: 931904 End: 937641 |
| Description | 1,4-alpha-glucan-branching enzyme, chloroplast precursor, putative, expressed |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH13 | 318 | 639 | 3e-29 |
| LPRIKKLGYNAVQIMAIQEHSYYASFGYHVTNFFAPSSRCGTPEELKSLIDRAHELGLVVLMDIVHSHASKNTLDGLNMFDGTDAHYFHSGPRGYHWMWD SRLFNYGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLSVGFTGNYTEYFGLETDVDAVNYLMLVNDMIHGLYPEAITVGEDVSGMPTFCIP VQDGGVGFDYRLHMAIADKWIEMLKKRDEDWQMGDIIYTLTNRRWSEKCISYAESHDQALVGDKTIAFWLMDKDMYDFMAVDRPSTPLIDRGIALHKMIR LITMGLGGEGYLNFMGNEFGHP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 806 Download |
| MVVIHGVSLT PRFTLPSRPL NTGFNAGNST LSFFFKKHPL SRKIFAGKQS AEFDSSSQAI 60 SASEKVLVPD NLDDDPRGFS QIFDLESQTM EYTEAVRTED QTMNVVKERG VKPRIVPPPG 120 DGKKIYEIDP MLRTYNNHLD YRYGQYKRLR EEIDKYEGGL EAFSRGYEKL GFSRSDAGIT 180 YREWAPGAKA ASLIGDFNNW NSNADIMTRN EFGVWEIFLP NNTDGSPAIP HGSRVKIRMD 240 TPSGIKDSIP AWIKFSVQAP GEIPFNGIYY DPPEEEKYVF KHPQPKRPKS LRIYEAHVGM 300 SSTEPMVNTY ANFRDDVLPR IKKLGYNAVQ IMAIQEHSYY ASFGYHVTNF FAPSSRCGTP 360 EELKSLIDRA HELGLVVLMD IVHSHASKNT LDGLNMFDGT DAHYFHSGPR GYHWMWDSRL 420 FNYGSWEVLR YLLSNARWWL EEYKFDGFRF DGVTSMMYTH HGLSVGFTGN YTEYFGLETD 480 VDAVNYLMLV NDMIHGLYPE AITVGEDVSG MPTFCIPVQD GGVGFDYRLH MAIADKWIEM 540 LKKRDEDWQM GDIIYTLTNR RWSEKCISYA ESHDQALVGD KTIAFWLMDK DMYDFMAVDR 600 PSTPLIDRGI ALHKMIRLIT MGLGGEGYLN FMGNEFGHPE WIDFPRGEQR LSDGSVIPGN 660 NFSYDKCRRR FDLGDADYLR YRGLQEFDQA MQHLEENYGF MTSEHQFISR KDEADRVIVF 720 ERGDLVFVFN FHWTSSYFDY RIGCSKPGKY KIVLDSDDPL FGGFNRLDRK AEYFTYDGLY 780 DERPCSFMVY APCRTAVVYA LANHD* 840 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN02960 | PLN02960 | 3.0e-9 | 133 | 219 | 93 | + alpha-amylase | ||
| PLN03244 | PLN03244 | 2.0e-136 | 227 | 802 | 587 | + alpha-amylase; Provisional | ||
| PLN02447 | PLN02447 | 0 | 61 | 805 | 749 | + 1,4-alpha-glucan-branching enzyme | ||
| cd11321 | AmyAc_bac_euk_BE | 0 | 274 | 689 | 417 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| PLN02960 | PLN02960 | 0 | 227 | 802 | 580 | + alpha-amylase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0003844 | 1,4-alpha-glucan branching enzyme activity |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| GO:0005982 | starch metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAB03100.1 | 0 | 8 | 805 | 3 | 800 | starch branching enzyme class II [Arabidopsis thaliana] |
| GenBank | ABN05322.1 | 0 | 31 | 802 | 36 | 824 | starch branching enzyme II [Populus trichocarpa] |
| GenBank | ABO31358.1 | 0 | 31 | 802 | 33 | 839 | starch branching enzyme II-1 [Malus x domestica] |
| GenBank | ABO31359.1 | 0 | 28 | 801 | 29 | 838 | starch branching enzyme II-2 [Malus x domestica] |
| RefSeq | NP_195985.3 | 0 | 1 | 805 | 1 | 805 | SBE2.2 (starch branching enzyme 2.2); 1,4-alpha-glucan branching enzyme [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3amk_A | 0 | 121 | 805 | 9 | 696 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 3vu2_B | 0 | 121 | 805 | 9 | 696 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 3vu2_A | 0 | 121 | 805 | 9 | 696 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 3aml_A | 0 | 125 | 805 | 13 | 696 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 3k1d_A | 0 | 106 | 797 | 81 | 717 | A Chain A, Crystal Structure Of Glycogen Branching Enzyme Synonym: 1,4- Glucan:1,4-Alpha-D-Glucan 6-Glucosyl-Transferase From Mycob Tuberculosis H3 |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch biosynthesis | RXN-7710 | EC-2.4.1.18 | 1,4-alpha-glucan branching enzyme |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO794536 | 685 | 119 | 803 | 0 |
| HO777638 | 629 | 175 | 803 | 0 |
| HO458123 | 393 | 408 | 800 | 0 |
| HO458123 | 294 | 119 | 407 | 0 |
| HO777638 | 47 | 128 | 174 | 0.000000002 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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