| Basic Information | |
|---|---|
| Species | Arabidopsis thaliana |
| Cazyme ID | AT5G07830.1 |
| Family | GH79 |
| Protein Properties | Length: 544 Molecular Weight: 60250.3 Isoelectric Point: 9.1953 |
| Chromosome | Chromosome/Scaffold: 5 Start: 2503323 End: 2506688 |
| Description | heparanase-like protein precursor, putative, expressed |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH79 | 44 | 537 | 0 |
| DENFVCATLDWWPHDKCNYDQCPWGYSSVINMDLTRPLLTKAIKAFKPLRIRIGGSLQDQVIYDVGNLKTPCRPFQKMNSGLFGFSKGCLHMKRWDELNS FLTATGAVVTFGLNALRGRHKLRGKAWGGAWDHINTQDFLNYTVSKGYVIDSWEFGNELSGSGVGASVSAELYGKDLIVLKDVINKVYKNSWLHKPILVA PGGFYEQQWYTKLLEISGPSVVDVVTHHIYNLGSGNDPALVKKIMDPSYLSQVSKTFKDVNQTIQEHGPWASPWVGESGGAYNSGGRHVSDTFIDSFWYL DQLGMSARHNTKVYCRQTLVGGFYGLLEKGTFVPNPDYYSALLWHRLMGKGVLAVQTDGPPQLRVYAHCSKGRAGVTLLLINLSNQSDFTVSVSNGINVV LNAESRKKKSLLDTLKRPFSWIGSKASDGYLNREEYHLTPENGVLRSKTMVLNGKSLKPTATGDIPSLEPVLRSVNSPLNVLPLSMSFIVLPNF | |||
| Full Sequence |
|---|
| Protein Sequence Length: 544 Download |
| MGFRVCVIVV FLGCLLLVPE KTMAQEMKRA SIVIQGARRV CETDENFVCA TLDWWPHDKC 60 NYDQCPWGYS SVINMDLTRP LLTKAIKAFK PLRIRIGGSL QDQVIYDVGN LKTPCRPFQK 120 MNSGLFGFSK GCLHMKRWDE LNSFLTATGA VVTFGLNALR GRHKLRGKAW GGAWDHINTQ 180 DFLNYTVSKG YVIDSWEFGN ELSGSGVGAS VSAELYGKDL IVLKDVINKV YKNSWLHKPI 240 LVAPGGFYEQ QWYTKLLEIS GPSVVDVVTH HIYNLGSGND PALVKKIMDP SYLSQVSKTF 300 KDVNQTIQEH GPWASPWVGE SGGAYNSGGR HVSDTFIDSF WYLDQLGMSA RHNTKVYCRQ 360 TLVGGFYGLL EKGTFVPNPD YYSALLWHRL MGKGVLAVQT DGPPQLRVYA HCSKGRAGVT 420 LLLINLSNQS DFTVSVSNGI NVVLNAESRK KKSLLDTLKR PFSWIGSKAS DGYLNREEYH 480 LTPENGVLRS KTMVLNGKSL KPTATGDIPS LEPVLRSVNS PLNVLPLSMS FIVLPNFDAS 540 ACS* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam03662 | Glyco_hydro_79n | 0 | 27 | 347 | 321 | + Glycosyl hydrolase family 79, N-terminal domain. Family of endo-beta-N-glucuronidase, or heparanase. Heparan sulfate proteoglycans (HSPGs) play a key role in the self- assembly, insolubility and barrier properties of basement membranes and extracellular matrices. Hence, cleavage of heparan sulfate (HS) affects the integrity and functional state of tissues and thereby fundamental normal and pathological phenomena involving cell migration and response to changes in the extracellular micro-environment. Heparanase degrades HS at specific intra-chain sites. The enzyme is synthesised as a latent approximately 65 kDa protein that is processed at the N-terminus into a highly active approximately 50 kDa form. Experimental evidence suggests that heparanase may facilitate both tumour cell invasion and neovascularization, both critical steps in cancer progression. The enzyme is also involved in cell migration associated with inflammation and autoimmunity. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004566 | beta-glucuronidase activity |
| GO:0005618 | cell wall |
| GO:0009505 | plant-type cell wall |
| GO:0016020 | membrane |
| GO:0016798 | hydrolase activity, acting on glycosyl bonds |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAB08480.1 | 0 | 27 | 543 | 1 | 516 | unnamed protein product [Arabidopsis thaliana] |
| EMBL | CAB62595.1 | 0 | 23 | 543 | 1 | 521 | putative protein [Arabidopsis thaliana] |
| RefSeq | NP_196400.2 | 0 | 1 | 543 | 1 | 543 | AtGUS2 (Arabidopsis thaliana glucuronidase 2); beta-glucuronidase |
| RefSeq | NP_200933.2 | 0 | 1 | 543 | 1 | 539 | AtGUS1 (Arabidopsis thaliana glucuronidase 1); beta-glucuronidase |
| RefSeq | XP_002514696.1 | 0 | 23 | 543 | 18 | 539 | Heparanase-2, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3vo0_A | 0.005 | 265 | 456 | 235 | 431 | A Chain A, Crystal Structure Of A Beta-Galactosidase From Bacteroides Thetaiotaomicron |
| PDB | 3vnz_A | 0.005 | 265 | 456 | 235 | 431 | A Chain A, Crystal Structure Of A Beta-Galactosidase From Bacteroides Thetaiotaomicron |
| PDB | 3vny_A | 0.005 | 265 | 456 | 235 | 431 | A Chain A, Crystal Structure Of Beta-Glucuronidase From Acidobacterium Capsulatum |
