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Basic Information | |
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Species | Arabidopsis thaliana |
Cazyme ID | AT5G11720.1 |
Family | GH31 |
Protein Properties | Length: 903 Molecular Weight: 101118 Isoelectric Point: 5.7228 |
Chromosome | Chromosome/Scaffold: 5 Start: 3776814 End: 3780146 |
Description | glycosyl hydrolase, family 31, putative, expressed |
View CDS |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH31 | 283 | 750 | 0 |
YVFAGPSPEMVMNQYTELIGRPAPMPYWSFGFHQCRYGYKNVSDLEYVVDGYAKAGIPLEVMWTDIDYMDGYKDFTLDPVNFPEDKMQSFVDTLHKNGQK YVLILDPGIGVDSSYGTYNRGMEADVFIKRNGEPYLGEVWPGKVYFPDFLNPAAATFWSNEIKMFQEILPLDGLWIDMNELSNFITSPLSSGSSLDDPPY KINNSGDKRPINNKTVPATSIHFGNISEYDAHNLYGLLEAKATHQAVVDITGKRPFILSRSTFVSSGKYTAHWTGDNAAKWEDLAYSIPGILNFGLFGIP MVGADICGFSHDTTEELCRRWIQLGAFYPFARDHSSLGTARQELYLWDSVASSARKVLGLRMRLLPHLYTLMYEAHVSGNPIARPLFFSFPQDTKTYEID SQFLIGKSIMVSPALKQGAVAVDAYFPAGNWFDLFNYSFAVGGDSGKHVRLDTPADHVNVHVREGSIV |
Full Sequence |
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Protein Sequence Length: 903 Download |
MSSLHWFPNI FIVVVVFFSL RSSQVVLEEE ESTVVGYGYV VRSVGVDSNR QVLTAKLDLI 60 KPSSVYAPDI KSLNLHVSLE TSERLRIRIT DSSQQRWEIP ETVIPRAGNH SPRRFSTEED 120 GGNSPENNFL ADPSSDLVFT LHNTTPFGFS VSRRSSGDIL FDTSPDSSDS NTYFIFKDQF 180 LQLSSALPEN RSNLYGIGEH TKRSFRLIPG ETMTLWNADI GSENPDVNLY GSHPFYMDVR 240 GSKGNEEAGT THGVLLLNSN GMDVKYEGHR ITYNVIGGVI DLYVFAGPSP EMVMNQYTEL 300 IGRPAPMPYW SFGFHQCRYG YKNVSDLEYV VDGYAKAGIP LEVMWTDIDY MDGYKDFTLD 360 PVNFPEDKMQ SFVDTLHKNG QKYVLILDPG IGVDSSYGTY NRGMEADVFI KRNGEPYLGE 420 VWPGKVYFPD FLNPAAATFW SNEIKMFQEI LPLDGLWIDM NELSNFITSP LSSGSSLDDP 480 PYKINNSGDK RPINNKTVPA TSIHFGNISE YDAHNLYGLL EAKATHQAVV DITGKRPFIL 540 SRSTFVSSGK YTAHWTGDNA AKWEDLAYSI PGILNFGLFG IPMVGADICG FSHDTTEELC 600 RRWIQLGAFY PFARDHSSLG TARQELYLWD SVASSARKVL GLRMRLLPHL YTLMYEAHVS 660 GNPIARPLFF SFPQDTKTYE IDSQFLIGKS IMVSPALKQG AVAVDAYFPA GNWFDLFNYS 720 FAVGGDSGKH VRLDTPADHV NVHVREGSIV AMQGEALTTR DARKTPYQLL VVASRLENIS 780 GELFLDDGEN LRMGAGGGNR DWTLVKFRCY VTGKSVVLRS EVVNPEYASK MKWSIGKVTF 840 VGFENVENVK TYEVRTSERL RSPRISLIKT VSDNDDPRFL SVEVSKLSLL VGKKFEMRLR 900 LT* 960 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd06600 | GH31_MGAM-like | 7.0e-112 | 302 | 661 | 363 | + This family includes the following closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), lysosomal acid alpha-glucosidase (GAA), neutral alpha-glucosidase C (GANC), the alpha subunit of neutral alpha-glucosidase AB (GANAB), and alpha-glucosidase II. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. Alpha-glucosidase II is a GH31 enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. The MGAM-like family corresponds to subgroup 1 in the Ernst et al classification of GH31 enzymes. | ||
cd06604 | GH31_glucosidase_II_MalA | 2.0e-122 | 302 | 661 | 366 | + Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. | ||
COG1501 | COG1501 | 1.0e-133 | 146 | 789 | 655 | + Alpha-glucosidases, family 31 of glycosyl hydrolases [Carbohydrate transport and metabolism] | ||
pfam01055 | Glyco_hydro_31 | 0 | 283 | 750 | 472 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
cd06602 | GH31_MGAM_SI_GAA | 0 | 302 | 679 | 383 | + This family includes the following three closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), and lysosomal acid alpha-glucosidase (GAA), also known as acid-maltase. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end, and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005773 | vacuole |
GO:0005975 | carbohydrate metabolic process |
GO:0009505 | plant-type cell wall |
GO:0048046 | apoplast |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAB82656.1 | 0 | 1 | 902 | 1 | 902 | alpha-glucosidase 1 [Arabidopsis thaliana] |
EMBL | CBI39013.1 | 0 | 11 | 902 | 902 | 1760 | unnamed protein product [Vitis vinifera] |
RefSeq | NP_196733.1 | 0 | 1 | 902 | 1 | 902 | alpha-glucosidase 1 (AGLU1) [Arabidopsis thaliana] |
RefSeq | XP_002317679.1 | 0 | 10 | 902 | 23 | 905 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002333838.1 | 0 | 29 | 902 | 27 | 896 | predicted protein [Populus trichocarpa] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3w38_A | 0 | 10 | 901 | 16 | 908 | A Chain A, Arabidopsis Thaliana Peroxidase A2 |
PDB | 3w37_A | 0 | 10 | 901 | 16 | 908 | A Chain A, Arabidopsis Thaliana Peroxidase A2 |
PDB | 3ctt_A | 0 | 53 | 849 | 65 | 827 | A Chain A, Arabidopsis Thaliana Peroxidase A2 |
PDB | 2qmj_A | 0 | 53 | 849 | 65 | 827 | A Chain A, Arabidopsis Thaliana Peroxidase A2 |
PDB | 2qly_A | 0 | 53 | 849 | 65 | 827 | A Chain A, Crystral Structure Of The N-Terminal Subunit Of Human Maltase- Glucoamylase |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
starch degradation I | RXN-2141 | EC-3.2.1.20 | α-glucosidase |