| Basic Information | |
|---|---|
| Species | Arabidopsis thaliana |
| Cazyme ID | AT5G11920.1 |
| Family | GH32 |
| Protein Properties | Length: 551 Molecular Weight: 62097.9 Isoelectric Point: 4.7932 |
| Chromosome | Chromosome/Scaffold: 5 Start: 3839391 End: 3842371 |
| Description | glycosyl hydrolases, putative, expressed |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 21 | 341 | 0 |
| HFQPQRNWLNDPNAPMYYKGFYHLFYQNNPLAPEFSRTRIIWGHSVSQDMVNWIQLEPALVPSESFDINSCWSGSATILPDGRPVILYTGLDVNNKQQVT VVAEPKDVSDPLLREWVKPKYNPVMVPPSNVPFNCFRDPTEAWKGQDGKWRVLIGAKEKDTEKGMAILYRSDDFVQWTKYPVPLLESEGTGMWECPDFFP VSITGKEGVDTSVNNASVRHVLKASFGGNDCYVIGKYSSETEDFSADYEFTNTSADLRYDHGTFYASKAFFDSVKNRRINWGWVIETDSKEDDFKKGWAG LMTLPREIWMDTSGKKLMQWP | |||
| Full Sequence |
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| Protein Sequence Length: 551 Download |
| MADVMEQNLL QTAVLNRTSF HFQPQRNWLN DPNAPMYYKG FYHLFYQNNP LAPEFSRTRI 60 IWGHSVSQDM VNWIQLEPAL VPSESFDINS CWSGSATILP DGRPVILYTG LDVNNKQQVT 120 VVAEPKDVSD PLLREWVKPK YNPVMVPPSN VPFNCFRDPT EAWKGQDGKW RVLIGAKEKD 180 TEKGMAILYR SDDFVQWTKY PVPLLESEGT GMWECPDFFP VSITGKEGVD TSVNNASVRH 240 VLKASFGGND CYVIGKYSSE TEDFSADYEF TNTSADLRYD HGTFYASKAF FDSVKNRRIN 300 WGWVIETDSK EDDFKKGWAG LMTLPREIWM DTSGKKLMQW PIEEINNLRT KSVSLDDCYE 360 FKTGSTFEIS GITAAQADVE VTFNLPFLEN NPEILDADQV DDATLFDRDS SVGCVYGPFG 420 LLALASSDLS EQTAIFFKVI RRGNGYAVVM CSSEKRSSLR DNIKKSSHGA FLDIDPRHEK 480 ISLRCLIDHS IIESYGVGGK TVITSRVYPK LAIGEAAKLY VFNDGENGVI MTSLEAWSMR 540 NAQINSNPTY * 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 1.0e-56 | 17 | 510 | 511 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 1.0e-62 | 17 | 509 | 510 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 2.0e-86 | 27 | 344 | 322 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 1.0e-134 | 21 | 341 | 330 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 4.0e-161 | 21 | 499 | 489 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| GO:0031219 | levanase activity |
| GO:0051670 | inulinase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAB87665.1 | 0 | 1 | 550 | 1 | 547 | fructosidase-like protein [Arabidopsis thaliana] |
| RefSeq | NP_001078574.1 | 0 | 1 | 410 | 1 | 410 | AtcwINV6 (6-&1-fructan exohydrolase); hydrolase, hydrolyzing O-glycosyl compounds / inulinase/ levanase [Arabidopsis thaliana] |
| RefSeq | NP_568254.1 | 0 | 1 | 550 | 1 | 550 | AtcwINV6 (6-&1-fructan exohydrolase); hydrolase, hydrolyzing O-glycosyl compounds / inulinase/ levanase [Arabidopsis thaliana] |
| RefSeq | XP_002309496.1 | 0 | 7 | 546 | 28 | 561 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002309497.1 | 0 | 9 | 550 | 39 | 572 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2aey_A | 0 | 17 | 544 | 8 | 533 | A Chain A, Structure And Activity Of A Flavonoid 3-O Glucosyltransferase Reveals The Basis For Plant Natural Product Modification |
| PDB | 2ade_A | 0 | 17 | 544 | 8 | 533 | A Chain A, Structure And Activity Of A Flavonoid 3-O Glucosyltransferase Reveals The Basis For Plant Natural Product Modification |
| PDB | 2add_A | 0 | 17 | 544 | 8 | 533 | A Chain A, Structure And Activity Of A Flavonoid 3-O Glucosyltransferase Reveals The Basis For Plant Natural Product Modification |
| PDB | 1st8_A | 0 | 17 | 544 | 8 | 533 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
| PDB | 2aez_A | 0 | 17 | 544 | 8 | 533 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| fructan degradation | RXN-1841 | EC-3.2.1.80 | fructan β-fructosidase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CT842376 | 545 | 15 | 545 | 0 |
| FD956604 | 220 | 278 | 497 | 0 |
| FD949297 | 205 | 14 | 215 | 0 |
| DY273985 | 324 | 36 | 355 | 0 |
| DR690484 | 299 | 24 | 320 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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