| Basic Information | |
|---|---|
| Species | Arabidopsis thaliana |
| Cazyme ID | AT5G26570.1 |
| Family | CBM20 |
| Protein Properties | Length: 1197 Molecular Weight: 131323 Isoelectric Point: 6.1594 |
| Chromosome | Chromosome/Scaffold: 5 Start: 9261580 End: 9267729 |
| Description | phosphoglucan, water dikinase, chloroplast precursor, putative, expressed |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
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| Family | Start | End | Evalue |
| CBM20 | 73 | 157 | 1.5e-21 |
| KVRLNVRLDHQVNFGDHVAMFGSAKEIGSWKKKSPLNWSENGWVCELELDGGQVLEYKFVIVKNDGSLSWESGDNRVLKVPNSGN | |||
| Full Sequence |
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| Protein Sequence Length: 1197 Download |
| MESIGSHCCS SPFTFITRNS SSSLPRLVNI THRVNLSHQS HRLRNSNSRL TCTATSSSTI 60 EEQRKKKDGS GTKVRLNVRL DHQVNFGDHV AMFGSAKEIG SWKKKSPLNW SENGWVCELE 120 LDGGQVLEYK FVIVKNDGSL SWESGDNRVL KVPNSGNFSV VCHWDATRET LDLPQEVGND 180 DDVGDGGHER DNHDVGDDRV VGSENGAQLQ KSTLGGQWQG KDASFMRSND HGNREVGRNW 240 DTSGLEGTAL KMVEGDRNSK NWWRKLEMVR EVIVGSVERE ERLKALIYSA IYLKWINTGQ 300 IPCFEDGGHH RPNRHAEISR LIFRELEHIC SKKDATPEEV LVARKIHPCL PSFKAEFTAA 360 VPLTRIRDIA HRNDIPHDLK QEIKHTIQNK LHRNAGPEDL IATEAMLQRI TETPGKYSGD 420 FVEQFKIFHN ELKDFFNAGS LTEQLDSMKI SMDDRGLSAL NLFFECKKRL DTSGESSNVL 480 ELIKTMHSLA SLRETIIKEL NSGLRNDAPD TAIAMRQKWR LCEIGLEDYF FVLLSRFLNA 540 LETMGGADQL AKDVGSRNVA SWNDPLDALV LGVHQVGLSG WKQEECLAIG NELLAWRERD 600 LLEKEGEEDG KTIWAMRLKA TLDRARRLTA EYSDLLLQIF PPNVEILGKA LGIPENSVKT 660 YTEAEIRAGI IFQISKLCTV LLKAVRNSLG SEGWDVVVPG STSGTLVQVE SIVPGSLPAT 720 SGGPIILLVN KADGDEEVSA ANGNIAGVML LQELPHLSHL GVRARQEKIV FVTCDDDDKV 780 ADIRRLVGKF VRLEASPSHV NLILSTEGRS RTSKSSATKK TDKNSLSKKK TDKKSLSIDD 840 EESKPGSSSS NSLLYSSKDI PSGGIIALAD ADVPTSGSKS AACGLLASLA EASSKVHSEH 900 GVPASFKVPT GVVIPFGSME LALKQNNSEE KFASLLEKLE TARPEGGELD DICDQIHEVM 960 KTLQVPKETI NSISKAFLKD ARLIVRSSAN VEDLAGMSAA GLYESIPNVS PSDPLVFSDS 1020 VCQVWASLYT RRAVLSRRAA GVSQREASMA VLVQEMLSPD LSFVLHTVSP ADPDSNLVEA 1080 EIAPGLGETL ASGTRGTPWR LASGKLDGIV QTLAFANFSE ELLVSGTGPA DGKYVRLTVD 1140 YSKKRLTVDS VFRQQLGQRL GSVGFFLERN FGCAQDVEGC LVGEDVYIVQ SRPQPL* 1200 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01418 | PEP_synth | 7.0e-25 | 872 | 1194 | 345 | + phosphoenolpyruvate synthase. Also called pyruvate,water dikinase and PEP synthase. The member from Methanococcus jannaschii contains a large intein. This enzyme generates phosphoenolpyruvate (PEP) from pyruvate, hydrolyzing ATP to AMP and releasing inorganic phosphate in the process. The enzyme shows extensive homology to other enzymes that use PEP as substrate or product. This enzyme may provide PEP for gluconeogenesis, for PTS-type carbohydrate transport systems, or for other processes [Energy metabolism, Glycolysis/gluconeogenesis]. | ||
| cd05467 | CBM20 | 2.0e-25 | 73 | 164 | 98 | + The family 20 carbohydrate-binding module (CBM20), also known as the starch-binding domain, is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch. | ||
| PRK06241 | PRK06241 | 3.0e-37 | 904 | 1193 | 296 | + phosphoenolpyruvate synthase; Validated | ||
| cd05818 | CBM20_water_dikinase | 4.0e-55 | 73 | 164 | 92 | + Phosphoglucan water dikinase (also known as alpha-glucan water dikinase), N-terminal CBM20 (carbohydrate-binding module, family 20) domain. This domain is found in the chloroplast-encoded phosphoglucan water dikinase, one of two enzymes involved in the phosphorylation of plant starches. In addition to the CBM20 domain, phosphoglucan water dikinase contains a C-terminal pyruvate binding domain. The CBM20 domain is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch. | ||
| pfam01326 | PPDK_N | 6.0e-56 | 870 | 1194 | 329 | + Pyruvate phosphate dikinase, PEP/pyruvate binding domain. This enzyme catalyzes the reversible conversion of ATP to AMP, pyrophosphate and phosphoenolpyruvate (PEP). | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005524 | ATP binding |
| GO:0005975 | carbohydrate metabolic process |
| GO:0005982 | starch metabolic process |
| GO:0005983 | starch catabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAU93516.1 | 0 | 1 | 1196 | 1 | 1196 | chloroplast alpha-glucan water dikinase isoform 3 [Arabidopsis thaliana] |
| RefSeq | NP_001119280.1 | 0 | 1 | 858 | 1 | 858 | ATGWD3; carbohydrate kinase/ catalytic/ phosphoglucan, water dikinase [Arabidopsis thaliana] |
| RefSeq | NP_198009.3 | 0 | 1 | 1196 | 1 | 1196 | ATGWD3; carbohydrate kinase/ catalytic/ phosphoglucan, water dikinase [Arabidopsis thaliana] |
| RefSeq | XP_002265211.1 | 0 | 40 | 1195 | 39 | 1187 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002518612.1 | 0 | 21 | 1195 | 10 | 1174 | chloroplast alpha-glucan water dikinase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2ols_A | 0.00000001 | 887 | 1196 | 25 | 338 | A Chain A, The Crystal Structure Of The Phosphoenolpyruvate Synthase From Neisseria Meningitidis |
| PDB | 2vn7_A | 0.000002 | 86 | 153 | 509 | 583 | A Chain A, The Crystal Structure Of The Phosphoenolpyruvate Synthase From Neisseria Meningitidis |
| PDB | 2vn4_A | 0.000002 | 86 | 153 | 509 | 583 | A Chain A, Glycoside Hydrolase Family 15 Glucoamylase From Hypocrea Jecorina |
| PDB | 1kum_A | 0.000002 | 72 | 168 | 5 | 108 | A Chain A, Glycoside Hydrolase Family 15 Glucoamylase From Hypocrea Jecorina |
| PDB | 1kul_A | 0.000002 | 72 | 168 | 5 | 108 | A Chain A, Glycoside Hydrolase Family 15 Glucoamylase From Hypocrea Jecorina |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
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| Hit | Length | Start | End | EValue |
| HO778000 | 893 | 317 | 1197 | 0 |
| HO794745 | 736 | 467 | 1197 | 0 |
| EX050720 | 284 | 911 | 1194 | 0 |
| EX122454 | 287 | 865 | 1151 | 0 |
| HO794745 | 45 | 424 | 468 | 0.000000001 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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