| Basic Information | |
|---|---|
| Species | Arabidopsis thaliana |
| Cazyme ID | AT5G26570.2 |
| Family | CBM20 |
| Protein Properties | Length: 866 Molecular Weight: 96315.8 Isoelectric Point: 6.8408 |
| Chromosome | Chromosome/Scaffold: 5 Start: 9261580 End: 9267729 |
| Description | phosphoglucan, water dikinase, chloroplast precursor, putative, expressed |
| View CDS | |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CBM20 | 73 | 157 | 9.5e-22 |
| KVRLNVRLDHQVNFGDHVAMFGSAKEIGSWKKKSPLNWSENGWVCELELDGGQVLEYKFVIVKNDGSLSWESGDNRVLKVPNSGN | |||
| Full Sequence |
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| Protein Sequence Length: 866 Download |
| MESIGSHCCS SPFTFITRNS SSSLPRLVNI THRVNLSHQS HRLRNSNSRL TCTATSSSTI 60 EEQRKKKDGS GTKVRLNVRL DHQVNFGDHV AMFGSAKEIG SWKKKSPLNW SENGWVCELE 120 LDGGQVLEYK FVIVKNDGSL SWESGDNRVL KVPNSGNFSV VCHWDATRET LDLPQEVGND 180 DDVGDGGHER DNHDVGDDRV VGSENGAQLQ KSTLGGQWQG KDASFMRSND HGNREVGRNW 240 DTSGLEGTAL KMVEGDRNSK NWWRKLEMVR EVIVGSVERE ERLKALIYSA IYLKWINTGQ 300 IPCFEDGGHH RPNRHAEISR LIFRELEHIC SKKDATPEEV LVARKIHPCL PSFKAEFTAA 360 VPLTRIRDIA HRNDIPHDLK QEIKHTIQNK LHRNAGPEDL IATEAMLQRI TETPGKYSGD 420 FVEQFKIFHN ELKDFFNAGS LTEQLDSMKI SMDDRGLSAL NLFFECKKRL DTSGESSNVL 480 ELIKTMHSLA SLRETIIKEL NSGLRNDAPD TAIAMRQKWR LCEIGLEDYF FVLLSRFLNA 540 LETMGGADQL AKDVGSRNVA SWNDPLDALV LGVHQVGLSG WKQEECLAIG NELLAWRERD 600 LLEKEGEEDG KTIWAMRLKA TLDRARRLTA EYSDLLLQIF PPNVEILGKA LGIPENSVKT 660 YTEAEIRAGI IFQISKLCTV LLKAVRNSLG SEGWDVVVPG STSGTLVQVE SIVPGSLPAT 720 SGGPIILLVN KADGDEEVSA ANGNIAGVML LQELPHLSHL GVRARQEKIV FVTCDDDDKV 780 ADIRRLVGKF VRLEASPSHV NLILSTEGRS RTSKSSATKK TDKNSLSKKK TDKKSLSIDD 840 EESKPGSSSS NSLLYSSKWR NHSTC* 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd02856 | E_set_GDE_Isoamylase_N | 3.0e-10 | 74 | 155 | 89 | + N-terminal Early set domain associated with the catalytic domain of Glycogen debranching enzyme and bacterial isoamylase (also called glycogen 6-glucanohydrolase). E or "early" set domains are associated with the catalytic domain of the glycogen debranching enzyme at the N-terminal end. Glycogen debranching enzymes have both 4-alpha-glucanotransferase and amylo-1,6-glucosidase activities. As a transferase, it transfers a segment of the 1,4-alpha-D-glucan to a new 4-position in an acceptor, which may be glucose or another 1,4-alpha-D-glucan. As a glucosidase, it catalyzes the endohydrolysis of 1,6-alpha-D-glucoside linkages at points of branching in chains of 1,4-linked alpha-D-glucose residues. Bacterial isoamylases are also included in this subfamily. Isoamylase is one of the starch-debranching enzymes that catalyze the hydrolysis of alpha-1,6-glucosidic linkages specific in alpha-glucans such as amylopectin or glycogen. Isoamylase contains a bound calcium ion, but this is not in the same position as the conserved calcium ion that has been reported in other alpha-amylase family enzymes. The N-terminal domain of glycogen debranching enzyme may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase, among others. | ||
| pfam00686 | CBM_20 | 2.0e-17 | 72 | 162 | 97 | + Starch binding domain. | ||
| smart01065 | CBM_2 | 8.0e-18 | 72 | 154 | 89 | + Starch binding domain. | ||
| cd05467 | CBM20 | 4.0e-25 | 73 | 164 | 98 | + The family 20 carbohydrate-binding module (CBM20), also known as the starch-binding domain, is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch. | ||
| cd05818 | CBM20_water_dikinase | 2.0e-54 | 73 | 164 | 92 | + Phosphoglucan water dikinase (also known as alpha-glucan water dikinase), N-terminal CBM20 (carbohydrate-binding module, family 20) domain. This domain is found in the chloroplast-encoded phosphoglucan water dikinase, one of two enzymes involved in the phosphorylation of plant starches. In addition to the CBM20 domain, phosphoglucan water dikinase contains a C-terminal pyruvate binding domain. The CBM20 domain is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005975 | carbohydrate metabolic process |
| GO:0005982 | starch metabolic process |
| GO:0005983 | starch catabolic process |
| GO:0009507 | chloroplast |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAU93516.1 | 0 | 1 | 858 | 1 | 858 | chloroplast alpha-glucan water dikinase isoform 3 [Arabidopsis thaliana] |
| EMBL | CBI39424.1 | 0 | 36 | 805 | 38 | 808 | unnamed protein product [Vitis vinifera] |
| RefSeq | NP_001119280.1 | 0 | 1 | 865 | 1 | 865 | ATGWD3; carbohydrate kinase/ catalytic/ phosphoglucan, water dikinase [Arabidopsis thaliana] |
| RefSeq | NP_198009.3 | 0 | 1 | 858 | 1 | 858 | ATGWD3; carbohydrate kinase/ catalytic/ phosphoglucan, water dikinase [Arabidopsis thaliana] |
| RefSeq | XP_002265211.1 | 0 | 36 | 805 | 38 | 808 | PREDICTED: hypothetical protein [Vitis vinifera] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1kum_A | 0.000001 | 72 | 168 | 5 | 108 | A Chain A, Glycoside Hydrolase Family 15 Glucoamylase From Hypocrea Jecorina |
| PDB | 1kul_A | 0.000001 | 72 | 168 | 5 | 108 | A Chain A, Glycoside Hydrolase Family 15 Glucoamylase From Hypocrea Jecorina |
| PDB | 1acz_A | 0.000001 | 72 | 168 | 5 | 108 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, 5 Structures |
| PDB | 1ac0_A | 0.000001 | 72 | 168 | 5 | 108 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, Minimized Average Structure |
| PDB | 2vn7_A | 0.000002 | 86 | 153 | 509 | 583 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, Minimized Average Structure |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation II | RXN-12204 | EC-2.7.9.5 | phosphoglucan, water dikinase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO778000 | 505 | 317 | 806 | 0 |
| EV093738 | 256 | 554 | 809 | 0 |
| HO794745 | 350 | 462 | 806 | 0 |
| DK535862 | 247 | 561 | 807 | 0 |
| HO794745 | 45 | 424 | 468 | 0.0000000008 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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