Basic Information | |
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Species | Aquilegia coerulea |
Cazyme ID | Aquca_001_00480.1 |
Family | AA7 |
Protein Properties | Length: 568 Molecular Weight: 62851.3 Isoelectric Point: 8.1617 |
Chromosome | Chromosome/Scaffold: 1 Start: 5051565 End: 5057261 |
Description | FAD-binding Berberine family protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
AA7 | 94 | 551 | 0 |
QIFTRSTFPRPLAIILPESKDQLANAISCCTKGSWTIRLRSGGHSYEGLSHIADSPFVIIDLMNLNGISIDLDTQTAWAESGATLGEIYHAIGASSNVLG FSAGYCPTVGSGGHISGGGYGMMSRKYGLAADNVVDALLVDGNGVVLDRKSMGEDVFWAIRGGGGGVWGAVYAWKLQLVPVPKNVTMFRLMKHTSEVEDA SKLIHKWQHVATKLEDDFSLAVLAGANKDSFWLLFLGLYLGPKELASSSIHQKFPELNLLLEDCMEMSWVEASAQLAGLKSVSELNDRFLKYDDRAFKTK VDFTKEAIPLEGIKGALEILKKEHRGFMVMNGQGGMMDKISTDATPFPHRSGTLSMIEYIVAWDKHEDLNSIEFINWLHQLYDYMGKFVSNNPRVGYVNH VDLELGTIDWANKTISSSRAIEIARTWGEKYFLSNYDRLVKAKTLIDPKNVFNHPQSI |
Full Sequence |
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Protein Sequence Length: 568 Download |
MGPRNNVRLC RMSNGKAAAG VVAKRKTREI MATGYRRFKA RGAVEVELVA TEVNRKMCAV 60 FQKINAFHCF HNVNLVDVTE KKIAWLLYVS MQNQIFTRST FPRPLAIILP ESKDQLANAI 120 SCCTKGSWTI RLRSGGHSYE GLSHIADSPF VIIDLMNLNG ISIDLDTQTA WAESGATLGE 180 IYHAIGASSN VLGFSAGYCP TVGSGGHISG GGYGMMSRKY GLAADNVVDA LLVDGNGVVL 240 DRKSMGEDVF WAIRGGGGGV WGAVYAWKLQ LVPVPKNVTM FRLMKHTSEV EDASKLIHKW 300 QHVATKLEDD FSLAVLAGAN KDSFWLLFLG LYLGPKELAS SSIHQKFPEL NLLLEDCMEM 360 SWVEASAQLA GLKSVSELND RFLKYDDRAF KTKVDFTKEA IPLEGIKGAL EILKKEHRGF 420 MVMNGQGGMM DKISTDATPF PHRSGTLSMI EYIVAWDKHE DLNSIEFINW LHQLYDYMGK 480 FVSNNPRVGY VNHVDLELGT IDWANKTISS SRAIEIARTW GEKYFLSNYD RLVKAKTLID 540 PKNVFNHPQS IPPLQGNHVG CIGNCDL* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
pfam08031 | BBE | 2.0e-14 | 490 | 552 | 63 | + Berberine and berberine like. This domain is found in the berberine bridge and berberine bridge- like enzymes which are involved in the biosynthesis of numerous isoquinoline alkaloids. They catalyze the transformation of the N-methyl group of (S)-reticuline into the C-8 berberine bridge carbon of (S)-scoulerine. | ||
pfam01565 | FAD_binding_4 | 2.0e-24 | 104 | 241 | 139 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
COG0277 | GlcD | 7.0e-25 | 103 | 554 | 474 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] |
Gene Ontology | |
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GO Term | Description |
GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
GO:0016491 | oxidoreductase activity |
GO:0050660 | flavin adenine dinucleotide binding |
GO:0055114 | oxidation-reduction process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3FW9 | 0 | 86 | 554 | 28 | 494 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With (S)- Scoulerine |
GenBank | AAD17487.1 | 0 | 86 | 554 | 49 | 516 | berberine bridge enzyme [Berberis stolonifera] |
GenBank | AAU20769.1 | 0 | 53 | 554 | 11 | 518 | berberine bridge enzyme [Thalictrum flavum subsp. glaucum] |
GenBank | ACJ76783.1 | 0 | 86 | 554 | 65 | 531 | berberine bridge enzyme 1 [Argemone mexicana] |
GenBank | ACJ76784.1 | 0 | 86 | 554 | 60 | 526 | berberine bridge enzyme 2 [Argemone mexicana] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3fw9_A | 0 | 86 | 554 | 28 | 494 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With (S)-Scoulerine |
PDB | 4ec3_A | 0 | 86 | 554 | 34 | 500 | A Chain A, Structure Of Berberine Bridge Enzyme, H174a Variant In Complex With (S)-Reticuline |
PDB | 3gsy_A | 0 | 86 | 554 | 34 | 500 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With Dehydroscoulerine |
PDB | 3d2j_A | 0 | 86 | 554 | 53 | 519 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With Dehydroscoulerine |
PDB | 3d2h_A | 0 | 86 | 554 | 53 | 519 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With Dehydroscoulerine |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
DR919677 | 231 | 334 | 564 | 0 |
DT740104 | 260 | 86 | 345 | 0 |
DT740103 | 209 | 354 | 562 | 0 |
JG654441 | 233 | 250 | 481 | 0 |
DT740104 | 37 | 346 | 382 | 0.0000002 |
Sequence Alignments (This image is cropped. Click for full image.) |
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