Basic Information | |
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Species | Aquilegia coerulea |
Cazyme ID | Aquca_008_00159.1 |
Family | PL4 |
Protein Properties | Length: 688 Molecular Weight: 79097.5 Isoelectric Point: 7.4625 |
Chromosome | Chromosome/Scaffold: 8 Start: 4294789 End: 4299857 |
Description | Rhamnogalacturonate lyase family protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
PL4 | 51 | 669 | 0 |
TDVKLEVGDDYVVIDNGLVRVTLSNPRGSVTRIQYNNVDNLLETHNEEENRGYWDLGWSKPEKAHHGIYDRIYGTNFTVIMKDPDQVELSFVRYWDPSFG NKSVPLNIDIRYVMLHGIPGLYSYAIYEHLKGWPDFDLAETRIVLKPSKDKFHYMAISDDRQRTMPMPEDRATGQTLGYKEAVRLTNPSNPELKGEVDDK YQYSCENKDCKVHGWISNDSFTGFWIIIPSNEFQSDGPFKQDLTSHVGPTTLVMFHSLHYSGEDVVLKFRDGEHWKKVFGPVFIYFNKVVDDHQEVPYSK LWKDAKNQMMNEVQSWPYQFPKSKDYPHLEQRGTVTGRLFVQDRYISKDYISAKSAYVGMALPGVAGSWEREGKGYQFWTKADASGSFCINNVREGKYSL YAYVPRFIGDYKYDVDITITPGCSCVIDVGDIVYKPPRNGPTVWEIGIADRSSGEFYIPDPAPKYINKLYLTRPNRRQYGLWERYTELYPDGLDILYVID ASDYSNDWFYAHVTRKNDNRTYKATTWQVLFELHNVDRNRGNYTLRLALASATYADLHVRVNNPQANPPLFKTGLIGKDNAIARHGIRGLYRLFNVNVPS KLLLKGSNSIYLTQSRGSS |
Full Sequence |
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Protein Sequence Length: 688 Download |
MQGFTKTNQI YVIDIGLWMM PWNPTLLFLT GKFLPGLDCR ESPAGKDKLA TDVKLEVGDD 60 YVVIDNGLVR VTLSNPRGSV TRIQYNNVDN LLETHNEEEN RGYWDLGWSK PEKAHHGIYD 120 RIYGTNFTVI MKDPDQVELS FVRYWDPSFG NKSVPLNIDI RYVMLHGIPG LYSYAIYEHL 180 KGWPDFDLAE TRIVLKPSKD KFHYMAISDD RQRTMPMPED RATGQTLGYK EAVRLTNPSN 240 PELKGEVDDK YQYSCENKDC KVHGWISNDS FTGFWIIIPS NEFQSDGPFK QDLTSHVGPT 300 TLVMFHSLHY SGEDVVLKFR DGEHWKKVFG PVFIYFNKVV DDHQEVPYSK LWKDAKNQMM 360 NEVQSWPYQF PKSKDYPHLE QRGTVTGRLF VQDRYISKDY ISAKSAYVGM ALPGVAGSWE 420 REGKGYQFWT KADASGSFCI NNVREGKYSL YAYVPRFIGD YKYDVDITIT PGCSCVIDVG 480 DIVYKPPRNG PTVWEIGIAD RSSGEFYIPD PAPKYINKLY LTRPNRRQYG LWERYTELYP 540 DGLDILYVID ASDYSNDWFY AHVTRKNDNR TYKATTWQVL FELHNVDRNR GNYTLRLALA 600 SATYADLHVR VNNPQANPPL FKTGLIGKDN AIARHGIRGL YRLFNVNVPS KLLLKGSNSI 660 YLTQSRGSSP FIGIMYDYIR LEGPPQK* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
pfam09284 | RhgB_N | 0.002 | 259 | 336 | 78 | + Rhamnogalacturonase B, N-terminal. Members of this family are found in prokaryotic Rhamnogalacturonase B, and adopt a structure consisting of a beta supersandwich, with eighteen strands in two beta-sheets. The exact function of the domain is unknown, but a putative role includes carbohydrate-binding. | ||
cd10316 | RGL4_M | 1.0e-29 | 381 | 482 | 102 | + Middle domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle domain represented by this model and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
cd10317 | RGL4_C | 5.0e-48 | 494 | 682 | 191 | + C-terminal domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
cd10320 | RGL4_N | 9.0e-70 | 53 | 344 | 296 | + N-terminal catalytic domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold; the middle and C-terminal domains are both putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
pfam06045 | Rhamnogal_lyase | 9.0e-82 | 57 | 247 | 193 | + Rhamnogalacturonate lyase family. Rhamnogalacturonate lyase (EC:4.2.2.-) degrades the rhamnogalacturonan I (RG-I) backbone of pectin. This family contains mainly members from plants, but also contains the plant pathogen Erwinia chrysanthemi. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CBI19298.1 | 0 | 53 | 685 | 6 | 645 | unnamed protein product [Vitis vinifera] |
RefSeq | NP_172460.6 | 0 | 64 | 684 | 1 | 616 | lyase [Arabidopsis thaliana] |
RefSeq | XP_002285626.1 | 0 | 64 | 686 | 1 | 617 | PREDICTED: hypothetical protein isoform 1 [Vitis vinifera] |
RefSeq | XP_002527352.1 | 0 | 53 | 684 | 6 | 631 | lyase, putative [Ricinus communis] |
RefSeq | XP_002527353.1 | 0 | 53 | 685 | 6 | 634 | lyase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3njv_A | 0.003 | 276 | 669 | 174 | 490 | A Chain A, Family Gh5 Endo-Beta-Mannanase From Lycopersicon Esculentum (Tomato) |
PDB | 2xhn_B | 0.003 | 276 | 669 | 174 | 490 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus K150a Active Site Mutant |
PDB | 2xhn_A | 0.003 | 276 | 669 | 174 | 490 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus K150a Active Site Mutant |
PDB | 1nkg_A | 0.003 | 276 | 669 | 174 | 490 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus |
PDB | 3njx_A | 0.003 | 276 | 669 | 174 | 490 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus Mutant H210a |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
GW864372 | 316 | 193 | 506 | 0 |
DY293973 | 361 | 50 | 406 | 0 |
JG640880 | 274 | 201 | 474 | 0 |
DW479599 | 290 | 53 | 339 | 0 |
DW479600 | 292 | 53 | 341 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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