y
Basic Information | |
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Species | Aquilegia coerulea |
Cazyme ID | Aquca_013_00697.2 |
Family | GH32 |
Protein Properties | Length: 604 Molecular Weight: 66784.4 Isoelectric Point: 5.0021 |
Chromosome | Chromosome/Scaffold: 13 Start: 4539030 End: 4542860 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 126 | 397 | 0 |
STNMLSWQRTAYHFQPQKNWMNDPDGSATLLPDGSIVMIYTGSTNESVQVQNVAFPANLSDPLLIDWFKYSGNPVLVPPPGIGAKDFRDPTTALQVSDKW RIVIGSRFNTTGIALVYETTDFLSYKLLDGLLHEVPTTGMWECVDFYPVSTTAANGLDTSVNGPGVKHVLKASLDDTKKDYYALGTYDSETNTWTPDDPE LDVGIGIRYDYGKYYASKTFYDQNKKRRILWSWIGETDSETADIKKRWSGVQGIPRVVTFDKKTKTNVLQWP |
Full Sequence |
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Protein Sequence Length: 604 Download |
MVEMMKNPFF SFPDLENEYP SSYTPLLQDQ ISKQDHERPR NVQQTSNKGL LLTFSLFICI 60 AFVVVFTGSD HSSMSPVVVI DDDNRPSLSS PSEKLTPIIP RGVSEGVSEK SFHPVSGDGI 120 LLFPWSTNML SWQRTAYHFQ PQKNWMNDPD GSATLLPDGS IVMIYTGSTN ESVQVQNVAF 180 PANLSDPLLI DWFKYSGNPV LVPPPGIGAK DFRDPTTALQ VSDKWRIVIG SRFNTTGIAL 240 VYETTDFLSY KLLDGLLHEV PTTGMWECVD FYPVSTTAAN GLDTSVNGPG VKHVLKASLD 300 DTKKDYYALG TYDSETNTWT PDDPELDVGI GIRYDYGKYY ASKTFYDQNK KRRILWSWIG 360 ETDSETADIK KRWSGVQGIP RVVTFDKKTK TNVLQWPVQE VESLRHSSVE FDKVKVEAGS 420 IIPLDVGTAT QLDIIADLEI DSKALEGTIE ADVIYNCSTS NGAAGRGALG PFGLLVLADE 480 SRSEQTAIYF YVAKGIDGHL TTFFCTDKSR SSKANDVGKE VYGSIVPVLD GERLTMRILV 540 DHSITEAFAQ KGRTCITSRV YPTQAIDGAA RLFLFNNATQ ASVTVTSLKI WHMKSASIHP 600 YPL* 660 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 6.0e-22 | 126 | 562 | 460 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 2.0e-34 | 134 | 595 | 538 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 3.0e-45 | 126 | 400 | 288 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 2.0e-89 | 138 | 397 | 326 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 6.0e-128 | 138 | 552 | 482 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABI17894.1 | 0 | 98 | 602 | 20 | 584 | vacuolar invertase [Coffea canephora] |
DDBJ | BAB82419.1 | 0 | 87 | 602 | 74 | 645 | acid invertase [Citrus unshiu] |
DDBJ | BAF34362.1 | 0 | 87 | 602 | 74 | 645 | beta-fructofuranosidase [Citrus sinensis] |
Swiss-Prot | P80065 | 0 | 83 | 603 | 83 | 658 | INVB_DAUCA RecName: Full=Beta-fructofuranosidase, soluble isoenzyme I; AltName: Full=Sucrose hydrolase; AltName: Full=Invertase; AltName: Full=Saccharase; Flags: Precursor |
RefSeq | XP_002303519.1 | 0 | 82 | 601 | 74 | 657 | predicted protein [Populus trichocarpa] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 118 | 601 | 3 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
PDB | 3ugh_A | 0 | 118 | 601 | 3 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
PDB | 3ugg_B | 0 | 118 | 601 | 3 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
PDB | 3ugg_A | 0 | 118 | 601 | 3 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
PDB | 3ugf_B | 0 | 118 | 601 | 3 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |