Basic Information | |
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Species | Aquilegia coerulea |
Cazyme ID | Aquca_015_00400.2 |
Family | GH13 |
Protein Properties | Length: 817 Molecular Weight: 93010.9 Isoelectric Point: 4.9214 |
Chromosome | Chromosome/Scaffold: 15 Start: 3840749 End: 3849892 |
Description | starch branching enzyme 2.1 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH13 | 275 | 600 | 1.8e-29 |
LPRIQANNYNTVQLMAVMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHSLGLRVLMDVVHSHASNNVTDGLNGFDVGQSAQESYFHAGDRGYHK LWDSRLFNYANWEVLRFLLSNLRWWLEEFKFDGFRFDGVTSMLYHHHGINMAFTGDYNEYFSESTDVDAVVYLMLANLVIHKILPDATVIAEDVSGMPAL GRLVSEGGIGFDYRLAMAIPDKWIDYVMNKKDEEWSMNDIVLTLTNRRYTEKCISYAESHDQSIVGDKTIAFSLMDKEMYSGMSCLTDASPTVERGVALH KMIHFITMALGGDGYLIFMGNEFGHP |
Full Sequence |
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Protein Sequence Length: 817 Download |
MHDFYFSCYH PQACRDHRCI TRRTRAAHGS MEFFGKLSFL SSQTLTVHGK VERNVAFATL 60 LDNENPTMTS SQDETENIAI MGLDPGLEGF KDHLKYRTQK YVEQKNLIEN FEGSLEDFAQ 120 GYLKFGFNRG QDCIVYREWA PAAQEAQLIG DFNGWDGFKH QMEKDQFGVW TLKIPNSGGT 180 PAIPHNSRVK FRFRHGDGTW VDRIPAWIKY ATVDPTKFAA AYDGVYWDPT PPERYEFKYP 240 RPPKPMSPRI YEAHVGMSSS EPCINSYREF ADNVLPRIQA NNYNTVQLMA VMEHSYYASF 300 GYHVTNFFAV SSRSGTPEDL KYLIDKAHSL GLRVLMDVVH SHASNNVTDG LNGFDVGQSA 360 QESYFHAGDR GYHKLWDSRL FNYANWEVLR FLLSNLRWWL EEFKFDGFRF DGVTSMLYHH 420 HGINMAFTGD YNEYFSESTD VDAVVYLMLA NLVIHKILPD ATVIAEDVSG MPALGRLVSE 480 GGIGFDYRLA MAIPDKWIDY VMNKKDEEWS MNDIVLTLTN RRYTEKCISY AESHDQSIVG 540 DKTIAFSLMD KEMYSGMSCL TDASPTVERG VALHKMIHFI TMALGGDGYL IFMGNEFGHP 600 DWIDFPREGN GWSYEKCRRQ WSLVDADHLR YKFLNAFNRA INSLDDRFSF LSSTKQIVSS 660 SNEEEKIIVF ERGDLVFVFN FHPENSYDGY KIGCDLPGKY RVALDSDAWE FGGHGRVAHD 720 VDHFTSPEGI PGVPETNFNG RPNSFKVLSP PRTCVVYYKV EEDLDDGYGE DFTGILDPVT 780 GAVALEENSD KSASSQEDVG PTPEDMEFQM GDESDD* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PLN02960 | PLN02960 | 2.0e-6 | 71 | 174 | 119 | + alpha-amylase | ||
PLN03244 | PLN03244 | 1.0e-136 | 181 | 757 | 581 | + alpha-amylase; Provisional | ||
PLN02447 | PLN02447 | 0 | 64 | 766 | 703 | + 1,4-alpha-glucan-branching enzyme | ||
cd11321 | AmyAc_bac_euk_BE | 0 | 231 | 639 | 409 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN02960 | PLN02960 | 0 | 181 | 758 | 581 | + alpha-amylase |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABN05321.1 | 0 | 13 | 800 | 25 | 814 | starch branching enzyme I [Populus trichocarpa] |
GenBank | ACM07441.1 | 0 | 12 | 766 | 25 | 784 | starch-branching enzyme I [Nelumbo nucifera] |
EMBL | CAA54308.1 | 0 | 15 | 807 | 30 | 822 | 1,4-alpha-glucan branching enzyme [Manihot esculenta] |
EMBL | CBI18866.1 | 0 | 19 | 787 | 34 | 811 | unnamed protein product [Vitis vinifera] |
RefSeq | XP_002284841.1 | 0 | 19 | 787 | 11 | 788 | PREDICTED: hypothetical protein [Vitis vinifera] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3aml_A | 0 | 68 | 813 | 1 | 752 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3amk_A | 0 | 68 | 766 | 1 | 699 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3vu2_B | 0 | 68 | 766 | 1 | 699 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 3vu2_A | 0 | 68 | 766 | 1 | 699 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 1m7x_D | 7.9874e-44 | 139 | 755 | 32 | 610 | A Chain A, The X-Ray Crystallographic Structure Of Branching Enzyme |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO619167 | 600 | 164 | 762 | 0 |
HO794536 | 719 | 80 | 786 | 0 |
HO777638 | 665 | 134 | 786 | 0 |
CX109187 | 443 | 377 | 813 | 0 |
HO777638 | 47 | 83 | 129 | 4.5 |
Sequence Alignments (This image is cropped. Click for full image.) |
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