y
Basic Information | |
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Species | Aquilegia coerulea |
Cazyme ID | Aquca_015_00400.4 |
Family | GH13 |
Protein Properties | Length: 793 Molecular Weight: 90251.8 Isoelectric Point: 4.9392 |
Chromosome | Chromosome/Scaffold: 15 Start: 3840311 End: 3849892 |
Description | starch branching enzyme 2.2 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH13 | 290 | 575 | 1.6e-26 |
LPRIQANNYNTVQLMAVMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHSLGLRVLMDVVHSHASNNVTDGLNGFDVGQSAQESYFHAGDRGYHK LWDSRLFNYANWEVLRFLLSNLRWWLEEFKFDGFRFDGVTSMLYHHHGINMAFTGDYNEYFSESTDVDAVVYLMLANLVIHKILPDATVIAEDVSGMPAL GRLVSEGGIGFDYRLAMAIPDKWIDYVMNKKDEEWSMNDIVLTLTNRRYTEKCISYAESHDQMIHFITMALGGDGYLIFMGNEFGH |
Full Sequence |
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Protein Sequence Length: 793 Download |
MMYCFSSSSS SFNLSLSLLP SNKINTKACR DHRCITRRTR AAHGSMEFFG KLSFLSSQTL 60 TVHGKVERNV AFATLLDNEN PTMTSSQDET ENIAIMGLDP GLEGFKDHLK YRTQKYVEQK 120 NLIENFEGSL EDFAQGYLKF GFNRGQDCIV YREWAPAAQE AQLIGDFNGW DGFKHQMEKD 180 QFGVWTLKIP NSGGTPAIPH NSRVKFRFRH GDGTWVDRIP AWIKYATVDP TKFAAAYDGV 240 YWDPTPPERY EFKYPRPPKP MSPRIYEAHV GMSSSEPCIN SYREFADNVL PRIQANNYNT 300 VQLMAVMEHS YYASFGYHVT NFFAVSSRSG TPEDLKYLID KAHSLGLRVL MDVVHSHASN 360 NVTDGLNGFD VGQSAQESYF HAGDRGYHKL WDSRLFNYAN WEVLRFLLSN LRWWLEEFKF 420 DGFRFDGVTS MLYHHHGINM AFTGDYNEYF SESTDVDAVV YLMLANLVIH KILPDATVIA 480 EDVSGMPALG RLVSEGGIGF DYRLAMAIPD KWIDYVMNKK DEEWSMNDIV LTLTNRRYTE 540 KCISYAESHD QMIHFITMAL GGDGYLIFMG NEFGHPDWID FPREGNGWSY EKCRRQWSLV 600 DADHLRYKFL NAFNRAINSL DDRFSFLSST KQIVSSSNEE EKIIVFERGD LVFVFNFHPE 660 NSYDGYKIGC DLPGKYRVAL DSDAWEFGGH GRVAHDVDHF TSPEGIPGVP ETNFNGRPNS 720 FKVLSPPRTC VVYYKVEEDL DDGYGEDFTG ILDPVTGAVA LEENSDKSAS SQEDVGPTPE 780 DMEFQMGDES DD* 840 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PLN02960 | PLN02960 | 2.0e-6 | 86 | 189 | 119 | + alpha-amylase | ||
PLN03244 | PLN03244 | 4.0e-125 | 196 | 733 | 574 | + alpha-amylase; Provisional | ||
PLN02960 | PLN02960 | 3.0e-174 | 196 | 734 | 576 | + alpha-amylase | ||
PLN02447 | PLN02447 | 0 | 79 | 742 | 703 | + 1,4-alpha-glucan-branching enzyme | ||
cd11321 | AmyAc_bac_euk_BE | 0 | 246 | 615 | 409 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABN05321.1 | 0 | 28 | 776 | 25 | 814 | starch branching enzyme I [Populus trichocarpa] |
GenBank | ACM07441.1 | 0 | 2 | 742 | 1 | 784 | starch-branching enzyme I [Nelumbo nucifera] |
EMBL | CAA54308.1 | 0 | 17 | 783 | 15 | 822 | 1,4-alpha-glucan branching enzyme [Manihot esculenta] |
EMBL | CBI18866.1 | 0 | 14 | 763 | 13 | 811 | unnamed protein product [Vitis vinifera] |
RefSeq | XP_002284841.1 | 0 | 34 | 763 | 11 | 788 | PREDICTED: hypothetical protein [Vitis vinifera] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3aml_A | 0 | 83 | 789 | 1 | 752 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3amk_A | 0 | 83 | 742 | 1 | 699 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3vu2_B | 0 | 83 | 742 | 1 | 699 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 3vu2_A | 0 | 83 | 742 | 1 | 699 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 1m7x_D | 4.06377e-44 | 154 | 731 | 32 | 610 | A Chain A, The X-Ray Crystallographic Structure Of Branching Enzyme |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO619167 | 600 | 179 | 738 | 0 |
HO794536 | 719 | 95 | 762 | 0 |
HO777638 | 665 | 149 | 762 | 0 |
HO458123 | 404 | 383 | 736 | 0 |
HO777638 | 47 | 98 | 144 | 4.2 |
Sequence Alignments (This image is cropped. Click for full image.) |
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