Basic Information | |
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Species | Aquilegia coerulea |
Cazyme ID | Aquca_027_00415.1 |
Family | GH13 |
Protein Properties | Length: 852 Molecular Weight: 96890.9 Isoelectric Point: 5.0129 |
Chromosome | Chromosome/Scaffold: 27 Start: 3334460 End: 3351617 |
Description | starch branching enzyme 2.2 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH13 | 366 | 686 | 9.4e-28 |
LPRIKMLGYNAVQLMAIQEHSYYASFGYHVTNFFAPSSRCGTPDDLKSLIDKAHELGLLVLMDIVHSHASNNVLDGLNMFDGTDGQYFHSGARGYHWMWD SRLFNYGSWEVLRFLLSNARWWLDEYKFDGFRFDGVTSMMYTHHGLQMAFTGNYDEYFGLATDVDAVVYLMLVNDLIHGLFPEAVTVGEDVSGMPTFGIP VQDGGVGFDYRLHMAVADKWIELLKKRDEDWQMGDIVHTLTNRRWSEKCVVYAESHDQALVGDKTIAFWLMDKDMYDFMALDRPSTPLIDRGIALHKMIR LITMGLGGEGYLNFMGNEFGH |
Full Sequence |
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Protein Sequence Length: 852 Download |
MAYSLSGVHL PTVPSICNSS RSSFLSNRKT SNLPILFKKN STCTWKICAG KSSYDSDSPS 60 ITVTTSEKVL VPGSKTDASA SAIEESEVVE EVDPLAMEDD VEVESTEELF SSELISVDKN 120 VDDEEETVSV NLVSEIGELE GARASNDLDA VKSGKAFRTT KRSIPPPGTG KKIYQIDPYL 180 ETHRPHLDYR FAQYKKMREE IDKYEGGLEI FSRGYEKLGF NRSTTGVTYR EWAPGAKSAS 240 LIGDFNNWNP NADVMTQNEF GVWEVFLPNN ADGSSPIPHG SRVKIHMDTP TGIKDSIPAW 300 IRFSVQAPGE IPYNGIYYDP PEEERYVFQH PRPKRPKSLR IYEAHVGMSS TEPKINTYAE 360 FRDDVLPRIK MLGYNAVQLM AIQEHSYYAS FGYHVTNFFA PSSRCGTPDD LKSLIDKAHE 420 LGLLVLMDIV HSHASNNVLD GLNMFDGTDG QYFHSGARGY HWMWDSRLFN YGSWEVLRFL 480 LSNARWWLDE YKFDGFRFDG VTSMMYTHHG LQMAFTGNYD EYFGLATDVD AVVYLMLVND 540 LIHGLFPEAV TVGEDVSGMP TFGIPVQDGG VGFDYRLHMA VADKWIELLK KRDEDWQMGD 600 IVHTLTNRRW SEKCVVYAES HDQALVGDKT IAFWLMDKDM YDFMALDRPS TPLIDRGIAL 660 HKMIRLITMG LGGEGYLNFM GNEFGHPEWI DFPRGEQHLP DGRVIPGNNN SYDKCRRRFD 720 LGDAEYLRYH GMQQFDQAMQ HLEDRYGFMT SQHQYISRKD EGDRVIVFER GDLVFVFNFH 780 WTTSYSDYRV GCLKPGKYKV VLDSDSKLFG GFNRIDPDAD YFSMEGMYDN RPNSFMLYTP 840 CRTAVVYALV D* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PLN02960 | PLN02960 | 6.0e-9 | 190 | 267 | 84 | + alpha-amylase | ||
PLN03244 | PLN03244 | 9.0e-139 | 277 | 849 | 581 | + alpha-amylase; Provisional | ||
PLN02447 | PLN02447 | 0 | 163 | 851 | 693 | + 1,4-alpha-glucan-branching enzyme | ||
cd11321 | AmyAc_bac_euk_BE | 0 | 322 | 737 | 417 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN02960 | PLN02960 | 0 | 277 | 850 | 579 | + alpha-amylase |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABO31358.1 | 0 | 1 | 851 | 1 | 840 | starch branching enzyme II-1 [Malus x domestica] |
GenBank | ABO31359.1 | 0 | 1 | 851 | 1 | 840 | starch branching enzyme II-2 [Malus x domestica] |
DDBJ | BAB64912.1 | 0 | 1 | 850 | 1 | 857 | starch branching enzyme II [Ipomoea batatas] |
EMBL | CBI30261.1 | 0 | 1 | 848 | 1 | 849 | unnamed protein product [Vitis vinifera] |
RefSeq | XP_002277213.1 | 0 | 31 | 848 | 239 | 1058 | PREDICTED: hypothetical protein [Vitis vinifera] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3amk_A | 0 | 173 | 851 | 13 | 694 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3aml_A | 0 | 173 | 851 | 13 | 694 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3vu2_B | 0 | 173 | 851 | 13 | 694 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 3vu2_A | 0 | 173 | 851 | 13 | 694 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 1m7x_D | 0 | 213 | 813 | 9 | 579 | A Chain A, The X-Ray Crystallographic Structure Of Branching Enzyme |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO794536 | 685 | 167 | 851 | 0 |
HO777638 | 629 | 223 | 851 | 0 |
HO458123 | 395 | 456 | 850 | 0 |
HO458123 | 296 | 167 | 455 | 0 |
HO777638 | 47 | 176 | 222 | 0.000000001 |
Sequence Alignments (This image is cropped. Click for full image.) |
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