y
Basic Information | |
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Species | Aquilegia coerulea |
Cazyme ID | Aquca_035_00234.1 |
Family | GH32 |
Protein Properties | Length: 657 Molecular Weight: 73771.8 Isoelectric Point: 5.3086 |
Chromosome | Chromosome/Scaffold: 35 Start: 2185492 End: 2188894 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 128 | 446 | 0 |
HFRPYRNWQSDPSGPMFYKGWYHFFYQHNPEAAVWGNIVWGHAVSRNLINWFYLPHALVPEHWYDIGGCFTGAATMLQDGSIVMLYTGATKDKVQIINQA YPADLSDPLLINWVKFSGNPILVQPPGIGLTEFRDPSTAWSTPTGTWRFTIGTKRNTTGISLMYETENFMNYTLLDEVLHGVPHTGMWECVDLYPVSLTG MNGLDTSVNGPLVKHVLKASIDEQQRDYYAVGSYDVKKDAWTPDDPDVDVGIELRYDYGKFYASRTFYDEKKQRRVLWGFSRETDSEDADRKKGWACLQI VPREITYDNKTKANVLQWP |
Full Sequence |
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Protein Sequence Length: 657 Download |
MDSIKNVEGA NTKSSYTTPL LDQTNSTKKN NWKPLKGLLI FSGVLLVLCI SVAFVINYNY 60 NYNYRSEDHV DEQNPKLETE EFIPQVVVVP EEDDAEKENR LFNDPGALHP SSYEWTNAML 120 SSQKSGFHFR PYRNWQSDPS GPMFYKGWYH FFYQHNPEAA VWGNIVWGHA VSRNLINWFY 180 LPHALVPEHW YDIGGCFTGA ATMLQDGSIV MLYTGATKDK VQIINQAYPA DLSDPLLINW 240 VKFSGNPILV QPPGIGLTEF RDPSTAWSTP TGTWRFTIGT KRNTTGISLM YETENFMNYT 300 LLDEVLHGVP HTGMWECVDL YPVSLTGMNG LDTSVNGPLV KHVLKASIDE QQRDYYAVGS 360 YDVKKDAWTP DDPDVDVGIE LRYDYGKFYA SRTFYDEKKQ RRVLWGFSRE TDSEDADRKK 420 GWACLQIVPR EITYDNKTKA NVLQWPVEEV ESLRSNKKEF SNVKVGAGSI VPLNVGKTTQ 480 LDITAEFEID KNALEGVEGG AVAYNCTTSH GASARSALGP FGLLVLANND HSEQTAVYFY 540 IAKGTDGNLR TFFCTDQSRS TKATDVRTEN YGSAVRVLDN EKFSVRILVD HSIVEAFAQG 600 GRTVITSRIY PTEAIEGAAR VFLFNNATRI GVTATSVKVW EMDSASLKFY PGLEKV* 660 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 1.0e-45 | 117 | 611 | 516 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 7.0e-68 | 127 | 617 | 504 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 2.0e-79 | 134 | 449 | 322 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 6.0e-128 | 128 | 446 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 3.0e-164 | 128 | 601 | 484 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABI17894.1 | 0 | 82 | 653 | 20 | 586 | vacuolar invertase [Coffea canephora] |
DDBJ | BAB82419.1 | 0 | 95 | 651 | 94 | 645 | acid invertase [Citrus unshiu] |
DDBJ | BAF34362.1 | 0 | 95 | 651 | 94 | 645 | beta-fructofuranosidase [Citrus sinensis] |
EMBL | CAA53099.1 | 0 | 112 | 651 | 119 | 657 | beta-fructofuranosidase [Daucus carota] |
RefSeq | XP_002303519.1 | 0 | 113 | 650 | 121 | 657 | predicted protein [Populus trichocarpa] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 113 | 648 | 8 | 542 | A Chain A, The Structure Of Alpha-N-Acetylgalactosaminidase |
PDB | 3ugh_A | 0 | 113 | 648 | 8 | 542 | A Chain A, The Structure Of Alpha-N-Acetylgalactosaminidase |
PDB | 3ugg_B | 0 | 113 | 648 | 8 | 542 | A Chain A, The Structure Of Alpha-N-Acetylgalactosaminidase |
PDB | 3ugg_A | 0 | 113 | 648 | 8 | 542 | A Chain A, The Structure Of Alpha-N-Acetylgalactosaminidase |
PDB | 3ugf_B | 0 | 113 | 648 | 8 | 542 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |