Basic Information | |
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Species | Aquilegia coerulea |
Cazyme ID | Aquca_039_00169.1 |
Family | GH32 |
Protein Properties | Length: 348 Molecular Weight: 38883.9 Isoelectric Point: 7.9529 |
Chromosome | Chromosome/Scaffold: 39 Start: 2006034 End: 2007683 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 1 | 133 | 8.1e-23 |
MWECVDLYPVSLTDTNGLDISVRGPHVKHVLKASIDEQQRDYYAIGSYNVTTDHWTPDDPAVDVGIELRYDYGKFYASKSFYDPIKQRRVLWGYVRETDS KTADKNKGWACVQIVPREIILDNKTMTNVLQWP |
Full Sequence |
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Protein Sequence Length: 348 Download |
MWECVDLYPV SLTDTNGLDI SVRGPHVKHV LKASIDEQQR DYYAIGSYNV TTDHWTPDDP 60 AVDVGIELRY DYGKFYASKS FYDPIKQRRV LWGYVRETDS KTADKNKGWA CVQIVPREII 120 LDNKTMTNVL QWPVKELESL RSNKKEFNPV KLGAGSIVPL KVGRQASQLD ITVDFEIDKN 180 ALEAATGQPG RVGYNCTRSH GAAGRSVLGP FGLLVHANDK RSEQTAIYFY VTKGTDGNLK 240 TFFCTDQSRS SKSTDITKEN YGSTVPVLDN EKFSLRVLVD HSIVEAFAMG GRRVITSRLY 300 PTEAIAGAER VFLFNNATAT SVTATSIKIW EMNPASMKFY FDDQKNS* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
pfam08244 | Glyco_hydro_32C | 7.0e-15 | 210 | 291 | 85 | + Glycosyl hydrolases family 32 C terminal. This domain corresponds to the C terminal domain of glycosyl hydrolase family 32. It forms a beta sandwich module. | ||
COG1621 | SacC | 3.0e-16 | 1 | 307 | 312 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 7.0e-20 | 1 | 136 | 137 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 4.0e-38 | 1 | 133 | 139 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 1.0e-76 | 1 | 291 | 295 | + Glycosyl hydrolases family 32. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABI17894.1 | 0 | 1 | 342 | 248 | 585 | vacuolar invertase [Coffea canephora] |
EMBL | CAA53098.1 | 0 | 1 | 342 | 321 | 659 | beta-fructofuranosidase [Daucus carota] |
EMBL | CAA53099.1 | 0 | 1 | 342 | 321 | 659 | beta-fructofuranosidase [Daucus carota] |
EMBL | CBI31885.1 | 0 | 1 | 346 | 130 | 471 | unnamed protein product [Vitis vinifera] |
Swiss-Prot | P80065 | 0 | 1 | 342 | 321 | 659 | INVB_DAUCA RecName: Full=Beta-fructofuranosidase, soluble isoenzyme I; AltName: Full=Sucrose hydrolase; AltName: Full=Invertase; AltName: Full=Saccharase; Flags: Precursor |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 1 | 338 | 209 | 542 | B Chain B, The Avrptob-Bak1 Complex Reveals Two Structurally Similar Kinaseinteracting Domains In A Single Type Iii Effector |
PDB | 3ugh_A | 0 | 1 | 338 | 209 | 542 | B Chain B, The Avrptob-Bak1 Complex Reveals Two Structurally Similar Kinaseinteracting Domains In A Single Type Iii Effector |
PDB | 3ugg_B | 0 | 1 | 338 | 209 | 542 | B Chain B, The Avrptob-Bak1 Complex Reveals Two Structurally Similar Kinaseinteracting Domains In A Single Type Iii Effector |
PDB | 3ugg_A | 0 | 1 | 338 | 209 | 542 | B Chain B, The Avrptob-Bak1 Complex Reveals Two Structurally Similar Kinaseinteracting Domains In A Single Type Iii Effector |
PDB | 3ugf_B | 0 | 1 | 338 | 209 | 542 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
DR928909 | 263 | 1 | 263 | 0 |
DR922887 | 265 | 5 | 269 | 0 |
JZ011116 | 255 | 86 | 340 | 0 |
JZ000784 | 239 | 1 | 239 | 0 |
JZ006248 | 236 | 5 | 240 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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