| Basic Information | |
|---|---|
| Species | Aquilegia coerulea |
| Cazyme ID | Aquca_043_00083.1 |
| Family | GH16 |
| Protein Properties | Length: 292 Molecular Weight: 32410 Isoelectric Point: 5.0486 |
| Chromosome | Chromosome/Scaffold: 43 Start: 1319009 End: 1320142 |
| Description | xyloglucan endotransglycosylase 6 |
| View CDS | |
| External Links |
|---|
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH16 | 52 | 206 | 3e-24 |
| NNGDLLPLSLDKASGSGFQSKNEYLFGKIDMQLKLVSGNSAGTVTAYYLSSQGTTHDEIDFEFLGNLTGDPYILHTNVFSQGKGNREQQFHLWFDPTADF HTYSILWNPQRIIFSVDGTPMREFKNSESIGNILSLWNADDWATRGGLVKTDWAH | |||
| Full Sequence |
|---|
| Protein Sequence Length: 292 Download |
| MAFSSWSSFS ISKVSSILLV CLMATTFMVA NASNFNQEFD ITWGDGRAKI VNNGDLLPLS 60 LDKASGSGFQ SKNEYLFGKI DMQLKLVSGN SAGTVTAYYL SSQGTTHDEI DFEFLGNLTG 120 DPYILHTNVF SQGKGNREQQ FHLWFDPTAD FHTYSILWNP QRIIFSVDGT PMREFKNSES 180 IGNILSLWNA DDWATRGGLV KTDWAHVPFT ASYQNFNADA CVWSSGASSC SSQSTTSAAA 240 NNAAWLSQEM DSTSQERMRW VQNKYRIYNY CTDVKRFPQG FPSECTSANL S* 300 |
| Functional Domains Download unfiltered results here | ||||||
|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description |
| cd02183 | GH16_fungal_CRH1_transglycosylase | 1.0e-22 | 76 | 218 | 165 | + glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. |
| cd00413 | Glyco_hydrolase_16 | 1.0e-22 | 42 | 198 | 177 | + glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. |
| pfam00722 | Glyco_hydro_16 | 1.0e-73 | 35 | 206 | 181 | + Glycosyl hydrolases family 16. |
| PLN03161 | PLN03161 | 6.0e-92 | 33 | 288 | 266 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional |
| cd02176 | GH16_XET | 1.0e-129 | 30 | 285 | 267 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. |
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005618 | cell wall |
| GO:0005975 | carbohydrate metabolic process |
| GO:0006073 | cellular glucan metabolic process |
| GO:0016762 | xyloglucan:xyloglucosyl transferase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAH36875.1 | 0 | 17 | 288 | 9 | 285 | xyloglucan endotransglucosylase/hydrolase [Rosa hybrid cultivar] |
| EMBL | CAN80250.1 | 0 | 15 | 291 | 4 | 287 | hypothetical protein [Vitis vinifera] |
| EMBL | CAN80252.1 | 0 | 17 | 291 | 4 | 287 | hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002270182.1 | 0 | 17 | 291 | 13 | 296 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002274601.1 | 0 | 10 | 291 | 7 | 297 | PREDICTED: hypothetical protein [Vitis vinifera] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1un1_B | 0 | 35 | 285 | 17 | 272 | A Chain A, Golgi Alpha-Mannosidase Ii |
| PDB | 1un1_A | 0 | 35 | 285 | 17 | 272 | A Chain A, Golgi Alpha-Mannosidase Ii |
| PDB | 1umz_B | 0 | 35 | 285 | 17 | 272 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 1umz_A | 0 | 35 | 285 | 17 | 272 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 2uwb_B | 0 | 35 | 276 | 20 | 259 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
