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Basic Information | |
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Species | Aquilegia coerulea |
Cazyme ID | Aquca_066_00053.1 |
Family | GH13 |
Protein Properties | Length: 891 Molecular Weight: 102862 Isoelectric Point: 6.5836 |
Chromosome | Chromosome/Scaffold: 66 Start: 1149363 End: 1160038 |
Description | Alpha amylase family protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH13 | 410 | 675 | 1.9e-22 |
FIENVLPHVKNAAYNAIQLMGVIEHKDYFTVGYRVTNLFAVSSRFGTPDDFKRLVDEAHGLGLLVLLDVAHSYSAADEMVGLSLFDGSNDCYFHTGKRGH HKYWGTRMFKYGDIDVLQYLLSNLKWWVSEYQIDGYQFHSISSMMYTHNGFASFTGDLEEYCNQYVDKDALIYLILANEMLHYLHPNIITIAEDATHYPG LCEPTSEGGLGFDYYVNLSVSEMWLWFLENVPDDQWSMNKIVNTLVGNRQSADKMLMYAENHNQSI |
Full Sequence |
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Protein Sequence Length: 891 Download |
MASFLLPSNL SFLSTDNFLP FQFQKKTITI STHKWKCSAT EQQKQGFNRK TTSSIDVDKG 60 TNPVGFLTKH GITHKAFAQF LRERHKAMKD LQDQIFNRFA NIMELASGYE LMGLHRNVQH 120 RADYMEWAPG ARYCALIGDF NGWSPTQDSA REGHFGHDDY GYWFIILEDK LRDGEKPDDY 180 YFQQYNYVDD YDKGDSGVTM EEIFKRMDEE YWEPGEDHYM KTRFEVPAKL YEQIFGPNGP 240 QTEEELPEIL DAKTRYEAYK EKHKDDPKSN LPPLDVIDNG KEYDIFNVVT DPAWKKKFRS 300 KKPPFPYWLE LRKGRKAWLK KYAPAVPHGS RYRVYFNTPS GSLERVPAWA TYVLPDVDGK 360 QSCAVHWDPP PEIAYKWKHM RPKKPNSLRI YECHVGISGS EPKISSFHEF IENVLPHVKN 420 AAYNAIQLMG VIEHKDYFTV GYRVTNLFAV SSRFGTPDDF KRLVDEAHGL GLLVLLDVAH 480 SYSAADEMVG LSLFDGSNDC YFHTGKRGHH KYWGTRMFKY GDIDVLQYLL SNLKWWVSEY 540 QIDGYQFHSI SSMMYTHNGF ASFTGDLEEY CNQYVDKDAL IYLILANEML HYLHPNIITI 600 AEDATHYPGL CEPTSEGGLG FDYYVNLSVS EMWLWFLENV PDDQWSMNKI VNTLVGNRQS 660 ADKMLMYAEN HNQSISGGQS FAEILFGDTK QNSGDSDYLL LRGSSLHKMI KLITFSIGGR 720 AYLNFMGNEF GHPKRVEFPM AGNDFSFSLA NRQWDLLMDE GIHNGLFSFD KDMMMLDEAQ 780 RVLSRGLPNV HHVNDATMVI SYIRGPFLFI FNFHPKGSYE NYIVGVEEAG EYQVILNTDE 840 IKYGGRGLLQ HEKYVQRTIS KRVDGFRNCL KVTLPSRSAQ VYKLTRILRI * 900 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PLN02447 | PLN02447 | 2.0e-11 | 77 | 177 | 101 | + 1,4-alpha-glucan-branching enzyme | ||
cd11321 | AmyAc_bac_euk_BE | 1.0e-170 | 371 | 771 | 407 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN02960 | PLN02960 | 0 | 19 | 890 | 878 | + alpha-amylase | ||
PLN03244 | PLN03244 | 0 | 34 | 890 | 862 | + alpha-amylase; Provisional | ||
PLN02447 | PLN02447 | 0 | 323 | 882 | 571 | + 1,4-alpha-glucan-branching enzyme |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
DDBJ | BAB02827.1 | 0 | 56 | 890 | 64 | 903 | starch-branching enzyme-like protein [Arabidopsis thaliana] |
EMBL | CBI26672.1 | 0 | 1 | 890 | 1 | 896 | unnamed protein product [Vitis vinifera] |
RefSeq | NP_001154629.1 | 0 | 56 | 890 | 64 | 899 | alpha-amylase/ catalytic/ cation binding [Arabidopsis thaliana] |
RefSeq | XP_002278858.1 | 0 | 1 | 890 | 1 | 866 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002529457.1 | 0 | 11 | 881 | 3 | 892 | 1,4-alpha-glucan branching enzyme, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3amk_A | 0 | 324 | 852 | 114 | 653 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3aml_A | 0 | 324 | 852 | 114 | 653 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3vu2_B | 0 | 324 | 852 | 114 | 653 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
JG636329 | 270 | 332 | 601 | 0 |
BF272517 | 278 | 345 | 622 | 0 |
CO104525 | 273 | 372 | 644 | 0 |
DY965821 | 293 | 254 | 546 | 0 |
EH718768 | 263 | 350 | 612 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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