Basic Information | |
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Species | Aquilegia coerulea |
Cazyme ID | Aquca_072_00084.2 |
Family | GH32 |
Protein Properties | Length: 514 Molecular Weight: 57797.4 Isoelectric Point: 7.6594 |
Chromosome | Chromosome/Scaffold: 72 Start: 559851 End: 563300 |
Description | cell wall invertase 2 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 1 | 309 | 0 |
MYYKGFYHLFYQYNPYGSTWGNIVWAHSYSKDLINWIPLEPAIYPSAPFDINGTWSGSATILPGNKPIILYTGLDTEYRQVQNIAFPKNASDPLLREWFK PDYNPVINPNKYMNATAFRDPTTAWYGKDGFWRVSVGHKRRNRGIAEVYRTRDFKTWTRGKHPLHSAADTGMWECPDFFPVSLKGTEGLETSVNGNHVKH ILKVSLDLTRFDIYMIGSYNAGKERFIPDATSVDNSTGLRYDYGNFYASKTFFDEKNNRRILFGWANESDTKIDDIRKGWAGIQVILFLYAIPRTLWLDQ SGKQLIQWP |
Full Sequence |
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Protein Sequence Length: 514 Download |
MYYKGFYHLF YQYNPYGSTW GNIVWAHSYS KDLINWIPLE PAIYPSAPFD INGTWSGSAT 60 ILPGNKPIIL YTGLDTEYRQ VQNIAFPKNA SDPLLREWFK PDYNPVINPN KYMNATAFRD 120 PTTAWYGKDG FWRVSVGHKR RNRGIAEVYR TRDFKTWTRG KHPLHSAADT GMWECPDFFP 180 VSLKGTEGLE TSVNGNHVKH ILKVSLDLTR FDIYMIGSYN AGKERFIPDA TSVDNSTGLR 240 YDYGNFYASK TFFDEKNNRR ILFGWANESD TKIDDIRKGW AGIQVILFLY AIPRTLWLDQ 300 SGKQLIQWPI KELESIRGNA VHMTNVPLAE GARIEIKGIT SAQADVEVTF SLPSLEYAEP 360 FDPTWVDAEQ ACGSKDPNTL AGIGPFGLLT LASQNVEEYT PVFFHVFKFE DKPVVLMCSG 420 GKMSSLDPDN YKPSFGGYVN VDVKDGSLSL RSLIDHSVVE SFGAGGKTCI TSRSYPTIAK 480 GANTHLYLFN NGSIPVTVKN LDAWSMGKPR MNI* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 1.0e-41 | 2 | 325 | 342 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 7.0e-51 | 1 | 477 | 495 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 2.0e-81 | 1 | 312 | 318 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 6.0e-131 | 1 | 309 | 317 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 5.0e-161 | 1 | 466 | 476 | + Glycosyl hydrolases family 32. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CAA49162.1 | 0 | 1 | 512 | 79 | 592 | beta-fructofuranosidase [Daucus carota] |
Swiss-Prot | P26792 | 0 | 1 | 512 | 79 | 592 | INV1_DAUCA RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme 1; AltName: Full=Sucrose hydrolase 1; AltName: Full=Invertase 1; AltName: Full=Cell wall beta-fructosidase 1; Flags: Precursor |
RefSeq | XP_002282171.1 | 0 | 1 | 512 | 1 | 505 | PREDICTED: hypothetical protein isoform 1 [Vitis vinifera] |
RefSeq | XP_002530337.1 | 0 | 1 | 512 | 67 | 573 | Beta-fructofuranosidase, insoluble isoenzyme 3 precursor, putative [Ricinus communis] |
RefSeq | XP_002530338.1 | 0 | 1 | 512 | 62 | 568 | Beta-fructofuranosidase, insoluble isoenzyme 1 precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2qqw_A | 0 | 1 | 512 | 24 | 537 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (D23a) From Arabidopsis Thaliana In Complex With Sucrose |
PDB | 2xqr_K | 0 | 1 | 512 | 24 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
PDB | 2xqr_I | 0 | 1 | 512 | 24 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
PDB | 2xqr_G | 0 | 1 | 512 | 24 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
PDB | 2xqr_E | 0 | 1 | 512 | 24 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
CT842376 | 520 | 1 | 512 | 0 |
DR943300 | 261 | 254 | 514 | 0 |
JZ007869 | 264 | 123 | 386 | 0 |
DR935393 | 239 | 276 | 514 | 0 |
DY273985 | 327 | 1 | 325 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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