| Basic Information | |
|---|---|
| Species | Aquilegia coerulea |
| Cazyme ID | Aquca_136_00003.4 |
| Family | GT20 |
| Protein Properties | Length: 933 Molecular Weight: 105204 Isoelectric Point: 6.2247 |
| Chromosome | Chromosome/Scaffold: 136 Start: 18237 End: 26285 |
| Description | trehalose-6-phosphate synthase |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 82 | 557 | 0 |
| ERNSGMPFKQRLLVVANRLPVSAVRRGEDSWSLEISAGGLVSALLGVKEFEAKWIGWAGVNVPDEIGQRALSKALAEKRCIPVFLDEEIVHQYYNGYCNN ILWPLFHYLGLPQEDRLATTRSFQSQFDAYKKANQMFADVVNEHYEEGDVVWCHDYHLMFLPKCLKEYNAKMKVGWFLHTPFPSSEIHRTLPSRSELLRS VLAADLVGFHTYDYARHFVSACTRILGLEGTPEGVEDQGKLTRVAAFPIGIDSERFIRALELPKVQDHIKELKERFSGRKVMLGVDRLDMIKGIPQKILA FEKFLEENTSWHDKVVLLQIAVPTRTDVPEYQKLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVACQD SKKGVLILSEFAGAAQSLGAGAILVNPWNITEVASSIGYALNMPAEEREKRHHHNFMHVTTHTAQDWAETFVSELN | |||
| Full Sequence |
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| Protein Sequence Length: 933 Download |
| MPGNKYNGHP SIPPNRVERL LRDRELRRFN RTFNSFEELS DGNRESELLE NDLRDDNNWD 60 VPPGEELFDA EAAVRALNDG YERNSGMPFK QRLLVVANRL PVSAVRRGED SWSLEISAGG 120 LVSALLGVKE FEAKWIGWAG VNVPDEIGQR ALSKALAEKR CIPVFLDEEI VHQYYNGYCN 180 NILWPLFHYL GLPQEDRLAT TRSFQSQFDA YKKANQMFAD VVNEHYEEGD VVWCHDYHLM 240 FLPKCLKEYN AKMKVGWFLH TPFPSSEIHR TLPSRSELLR SVLAADLVGF HTYDYARHFV 300 SACTRILGLE GTPEGVEDQG KLTRVAAFPI GIDSERFIRA LELPKVQDHI KELKERFSGR 360 KVMLGVDRLD MIKGIPQKIL AFEKFLEENT SWHDKVVLLQ IAVPTRTDVP EYQKLTSQVH 420 EIVGRINGRF GTLTAVPIHH LDRSLDFHAL CALYAVTDVA LVTSLRDGMN LVSYEFVACQ 480 DSKKGVLILS EFAGAAQSLG AGAILVNPWN ITEVASSIGY ALNMPAEERE KRHHHNFMHV 540 TTHTAQDWAE TFVSELNDTI VEAQLRMRQV PPLLPVEVAV QHYLGSRNRL LILGFNATLT 600 EPVETPGRRG GDQIKEMELK LHPELKEPLK ILCNDPKTTV VVVSGSDRSV LDDNFGDYNI 660 WLAAENGMFL RPTKGEWMTT MPEHLNMDWV DSVKHVFEYF TERTPRSHFE LRETSLVWNY 720 KYADVEFGRL QARDLLQHLW TGPISNAAVD VVQGSRSVEV RSVGVTKGAA VDRILGEIVH 780 SRSIISPIDY VLCIGHFLGK DEDIFTFFEP ELPAESSTAS RAKTTEAAKS PVERWSSEDL 840 SLGKNGSKTS HGRSHRATES LDRSRNNQTG GSNWRSPQEN ITWNEGSSVL DLRGDNYFSV 900 AVGRKRSNAR YLLGSADDTV TFLKMLAAAT SS* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03064 | PLN03064 | 0 | 1 | 932 | 936 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03063 | PLN03063 | 0 | 90 | 932 | 843 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| TIGR02400 | trehalose_OtsA | 0 | 92 | 558 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| pfam00982 | Glyco_transf_20 | 0 | 91 | 558 | 474 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| PRK14501 | PRK14501 | 0 | 92 | 818 | 734 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABO61742.1 | 0 | 1 | 932 | 1 | 922 | trehalose-phosphate synthase 1 [Solanum lycopersicum] |
| RefSeq | XP_002285632.1 | 0 | 1 | 932 | 1 | 926 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002331300.1 | 0 | 1 | 931 | 1 | 922 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002522296.1 | 0 | 1 | 931 | 1 | 913 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| RefSeq | XP_002531237.1 | 0 | 1 | 931 | 1 | 924 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 0 | 92 | 566 | 3 | 464 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 0 | 92 | 566 | 3 | 464 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 0 | 92 | 566 | 3 | 464 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 0 | 92 | 566 | 3 | 464 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1gz5_D | 0 | 92 | 560 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CX546763 | 305 | 78 | 381 | 0 |
| CV711307 | 293 | 90 | 382 | 0 |
| DW069910 | 288 | 178 | 465 | 0 |
| CB651133 | 281 | 118 | 398 | 0 |
| EL390904 | 268 | 295 | 562 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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