| Basic Information | |
|---|---|
| Species | Aquilegia coerulea |
| Cazyme ID | Aquca_136_00003.7 |
| Family | GT20 |
| Protein Properties | Length: 571 Molecular Weight: 63666.1 Isoelectric Point: 6.2675 |
| Chromosome | Chromosome/Scaffold: 136 Start: 18237 End: 23510 |
| Description | trehalose-6-phosphate synthase |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 1 | 195 | 0 |
| MLGVDRLDMIKGIPQKILAFEKFLEENTSWHDKVVLLQIAVPTRTDVPEYQKLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALV TSLRDGMNLVSYEFVACQDSKKGVLILSEFAGAAQSLGAGAILVNPWNITEVASSIGYALNMPAEEREKRHHHNFMHVTTHTAQDWAETFVSELN | |||
| Full Sequence |
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| Protein Sequence Length: 571 Download |
| MLGVDRLDMI KGIPQKILAF EKFLEENTSW HDKVVLLQIA VPTRTDVPEY QKLTSQVHEI 60 VGRINGRFGT LTAVPIHHLD RSLDFHALCA LYAVTDVALV TSLRDGMNLV SYEFVACQDS 120 KKGVLILSEF AGAAQSLGAG AILVNPWNIT EVASSIGYAL NMPAEEREKR HHHNFMHVTT 180 HTAQDWAETF VSELNDTIVE AQLRMRQVPP LLPVEVAVQH YLGSRNRLLI LGFNATLTEP 240 VETPGRRGGD QIKEMELKLH PELKEPLKIL CNDPKTTVVV VSGSDRSVLD DNFGDYNIWL 300 AAENGMFLRP TKGEWMTTMP EHLNMDWVDS VKHVFEYFTE RTPRSHFELR ETSLVWNYKY 360 ADVEFGRLQA RDLLQHLWTG PISNAAVDVV QGSRSVEVRS VGVTKGAAVD RILGEIVHSR 420 SIISPIDYVL CIGHFLGKDE DIFTFFEPEL PAESSTASRA KTTEAAKSPV ERWSSEDLSL 480 GKNGSKTSHG RSHRATESLD RSRNNQTGGS NWRSPQENIT WNEGSSVLDL RGDNYFSVAV 540 GRKRSNARYL LGSADDTVTF LKMLAAATSS * 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR02400 | trehalose_OtsA | 5.0e-114 | 1 | 196 | 196 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| PRK14501 | PRK14501 | 2.0e-127 | 2 | 456 | 456 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| PLN03064 | PLN03064 | 0 | 1 | 570 | 571 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03063 | PLN03063 | 0 | 1 | 570 | 570 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | EAY98715.1 | 0 | 1 | 570 | 275 | 838 | hypothetical protein OsI_20647 [Oryza sativa Indica Group] |
| RefSeq | XP_002285632.1 | 0 | 1 | 570 | 359 | 926 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002331300.1 | 0 | 1 | 569 | 359 | 922 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002522296.1 | 0 | 1 | 569 | 354 | 913 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| RefSeq | XP_002531237.1 | 0 | 1 | 569 | 362 | 924 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 4.99997e-41 | 1 | 204 | 258 | 464 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 4.99997e-41 | 1 | 204 | 258 | 464 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 4.99997e-41 | 1 | 204 | 258 | 464 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 4.99997e-41 | 1 | 204 | 258 | 464 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1gz5_D | 4.99997e-41 | 1 | 198 | 257 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| ES813375 | 362 | 91 | 452 | 0 |
| CX545499 | 306 | 33 | 338 | 0 |
| GE582665 | 283 | 66 | 348 | 0 |
| EL352176 | 252 | 1 | 252 | 0 |
| GO265628 | 304 | 85 | 388 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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