| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra000229 |
| Family | AA7 |
| Protein Properties | Length: 441 Molecular Weight: 49266.3 Isoelectric Point: 8.7706 |
| Chromosome | Chromosome/Scaffold: 03 Start: 10045984 End: 10047977 |
| Description | cytokinin oxidase/dehydrogenase 1 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 57 | 240 | 3.8e-27 |
| NRYQLPPLAILHPRSVSDISTMVRHIVHLGSTSNLTVAARGHGHSLQGQSLAHQGVVINMESLRSPDIKIHKGKQPYADVSGGESWINILRETLKYGLSP KSWTDYLHLTVGGTLSNAGISGQAFKYGPQINNVYQLEIITGKGEVMTCSEKQNSELFYSVLGGLGQFGIITRARIALGPAPHM | |||
| Full Sequence |
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| Protein Sequence Length: 441 Download |
| MILVLSCIPG RTNLCSNHSV TTPIDLPPDI RSSLDSLDLE GYISFNDVHN ASKDFGNRYQ 60 LPPLAILHPR SVSDISTMVR HIVHLGSTSN LTVAARGHGH SLQGQSLAHQ GVVINMESLR 120 SPDIKIHKGK QPYADVSGGE SWINILRETL KYGLSPKSWT DYLHLTVGGT LSNAGISGQA 180 FKYGPQINNV YQLEIITGKG EVMTCSEKQN SELFYSVLGG LGQFGIITRA RIALGPAPHM 240 EVEKLLSELS YIPSTLFSSE VPYIEFLDRV HIAEIKLRAK GLWEVPHPWL NLLIPKSSIF 300 EFATEVFNNI LTSNNNGPIL IYPVNQSKWN KHTSLITPAE DIFYLVAFLP SAVPNPNSRT 360 SNLEYLLRQN QRVLNFCSAA NINVKQYLPH YETQREWRSH FGNRWETFAK RKHTYDPLAI 420 LAPGHRIFQK ATQLSPIQLL * 480 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG0277 | GlcD | 2.0e-22 | 40 | 425 | 453 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam01565 | FAD_binding_4 | 4.0e-23 | 63 | 206 | 145 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| pfam09265 | Cytokin-bind | 8.0e-103 | 241 | 428 | 189 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
| PLN02441 | PLN02441 | 4.0e-119 | 239 | 435 | 197 | + cytokinin dehydrogenase | ||
| PLN02441 | PLN02441 | 2.0e-136 | 1 | 240 | 244 | + cytokinin dehydrogenase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009690 | cytokinin metabolic process |
| GO:0016491 | oxidoreductase activity |
| GO:0019139 | cytokinin dehydrogenase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAG16373.1 | 0 | 1 | 240 | 22 | 252 | cytokinin oxidase family protein [Brassica oleracea var. italica] |
| DDBJ | BAG16373.1 | 0 | 241 | 431 | 340 | 524 | cytokinin oxidase family protein [Brassica oleracea var. italica] |
| DDBJ | BAG16379.1 | 0 | 1 | 240 | 13 | 243 | cytokinin oxidase family protein [Brassica rapa var. perviridis] |
| RefSeq | NP_181682.1 | 0 | 1 | 240 | 22 | 265 | CKX1 (CYTOKININ OXIDASE/DEHYDROGENASE 1); cytokinin dehydrogenase [Arabidopsis thaliana] |
| RefSeq | NP_181682.1 | 0 | 241 | 439 | 353 | 551 | CKX1 (CYTOKININ OXIDASE/DEHYDROGENASE 1); cytokinin dehydrogenase [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2q4w_A | 0 | 35 | 265 | 32 | 275 | A Chain A, Characterization And Engineering Of The Bifunctional N- And O-glucosyltransferase Involved In Xenobiotic Metabolism In Plants |
| PDB | 2q4w_A | 0 | 242 | 431 | 335 | 523 | A Chain A, Characterization And Engineering Of The Bifunctional N- And O-glucosyltransferase Involved In Xenobiotic Metabolism In Plants |
| PDB | 2exr_A | 0 | 35 | 265 | 32 | 275 | A Chain A, X-Ray Structure Of Cytokinin OxidaseDEHYDROGENASE (CKX) FROM Arabidopsis Thaliana At5g21482 |
| PDB | 2exr_A | 0 | 242 | 431 | 335 | 523 | A Chain A, X-Ray Structure Of Cytokinin OxidaseDEHYDROGENASE (CKX) FROM Arabidopsis Thaliana At5g21482 |
| PDB | 3kjm_A | 0 | 32 | 238 | 18 | 228 | A Chain A, X-Ray Structure Of Cytokinin OxidaseDEHYDROGENASE (CKX) FROM Arabidopsis Thaliana At5g21482 |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cytokinins degradation | RXN-4621 | EC-1.5.99 | isopentenyladenine:FAD oxidoreductase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EV217277 | 189 | 1 | 189 | 0 |
| EB680403 | 241 | 7 | 238 | 0 |
| DR926188 | 199 | 42 | 240 | 0 |
| FS319654 | 242 | 1 | 240 | 0 |
| GR931884 | 223 | 1 | 223 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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