| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra003862 |
| Family | AA3 |
| Protein Properties | Length: 595 Molecular Weight: 65477.1 Isoelectric Point: 10.2903 |
| Chromosome | Chromosome/Scaffold: 07 Start: 15423224 End: 15426183 |
| Description | Glucose-methanol-choline (GMC) oxidoreductase family protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA3 | 43 | 587 | 0 |
| ASTFASSSSSSFSSNGRDSTYDYIVIGGGTAGCPLAATLSRNFSVLVLERGGVPFTNANVSFLRNFHIGLADTSASSASQAFVSTDGVYNARARVLGGGS CINAGFYSRADAAFVKRAGWDPKLVNESYPWVEREIVHQPKLTLWQKALRDSLLEVGVRPFNGYTYDHVSGTKIGGTIFDRFGRRHTAAELLAYANPQKL RVLIYATVQKIVFDTSGARPRVTGVIFKDENGNQHQALLSNRKGSEVILSSGAIGSPQMLMLSGIGPKRELQRLKIPLVLENEHVGKGMADNPMNTILVP SKQPIEQSLIQTVGITKMGVYVEASTGFGQSPESIHTHYGVMSDKNELFSTIPAKQRRPEATQAYIRRNKYQLHEAFNGSFILEKLAYPISRGHLSLLNT NVDDNPSVTFNYFKHPVDLQRCVEAIRLVSKVVTSKRFLNYTQCDKQNVHEMLSLSVKANINLRPKQVNDTKSMAQFCKDTVVTIWHYHGGCLVGKVVSP DRKVLGVNRLRVIDGSTFDESPGTNPQATVMMMGRYMGVKILRKR | |||
| Full Sequence |
|---|
| Protein Sequence Length: 595 Download |
| MALKLFLFAL LISLPTSLSS TASKGKEQKL KFNPYRYTFI DKASTFASSS SSSFSSNGRD 60 STYDYIVIGG GTAGCPLAAT LSRNFSVLVL ERGGVPFTNA NVSFLRNFHI GLADTSASSA 120 SQAFVSTDGV YNARARVLGG GSCINAGFYS RADAAFVKRA GWDPKLVNES YPWVEREIVH 180 QPKLTLWQKA LRDSLLEVGV RPFNGYTYDH VSGTKIGGTI FDRFGRRHTA AELLAYANPQ 240 KLRVLIYATV QKIVFDTSGA RPRVTGVIFK DENGNQHQAL LSNRKGSEVI LSSGAIGSPQ 300 MLMLSGIGPK RELQRLKIPL VLENEHVGKG MADNPMNTIL VPSKQPIEQS LIQTVGITKM 360 GVYVEASTGF GQSPESIHTH YGVMSDKNEL FSTIPAKQRR PEATQAYIRR NKYQLHEAFN 420 GSFILEKLAY PISRGHLSLL NTNVDDNPSV TFNYFKHPVD LQRCVEAIRL VSKVVTSKRF 480 LNYTQCDKQN VHEMLSLSVK ANINLRPKQV NDTKSMAQFC KDTVVTIWHY HGGCLVGKVV 540 SPDRKVLGVN RLRVIDGSTF DESPGTNPQA TVMMMGRYMG VKILRKRLGN KAGV* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR03970 | Rv0697 | 4.0e-19 | 64 | 335 | 314 | + dehydrogenase, Rv0697 family. This model describes a set of dehydrogenases belonging to the glucose-methanol-choline oxidoreductase (GMC oxidoreductase) family. Members of the present family are restricted to Actinobacterial genome contexts containing also members of families TIGR03962 and TIGR03969 (the mycofactocin system), and are proposed to be uniform in function. | ||
| pfam05199 | GMC_oxred_C | 7.0e-27 | 431 | 576 | 151 | + GMC oxidoreductase. This domain found associated with pfam00732. | ||
| TIGR01810 | betA | 3.0e-39 | 64 | 576 | 565 | + choline dehydrogenase. Choline dehydrogenase catalyzes the conversion of exogenously supplied choline into the intermediate glycine betaine aldehyde, as part of a two-step oxidative reaction leading to the formation of osmoprotectant betaine. This enzymatic system can be found in both gram-positive and gram-negative bacteria. As in Escherichia coli , Staphylococcus xylosus , and Sinorhizobium meliloti, this enzyme is found associated in a transciptionally co-induced gene cluster with betaine aldehyde dehydrogenase, the second catalytic enzyme in this reaction. Other gram-positive organisms have been shown to employ a different enzymatic system, utlizing a soluable choline oxidase or type III alcohol dehydrogenase instead of choline dehydrogenase. This enzyme is a member of the GMC oxidoreductase family (pfam00732 and pfam05199), sharing a common evoluntionary origin and enzymatic reaction with alcohol dehydrogenase. Outgrouping from this model, Caulobacter crescentus shares sequence homology with choline dehydrogenase, yet other genes participating in this enzymatic reaction have not currently been identified [Cellular processes, Adaptations to atypical conditions]. | ||
| COG2303 | BetA | 5.0e-46 | 59 | 585 | 575 | + Choline dehydrogenase and related flavoproteins [Amino acid transport and metabolism] | ||
| PLN02785 | PLN02785 | 0 | 26 | 594 | 569 | + Protein HOTHEAD | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0016614 | oxidoreductase activity, acting on CH-OH group of donors |
| GO:0050660 | flavin adenine dinucleotide binding |
| GO:0055114 | oxidation-reduction process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAA77842.1 | 0 | 15 | 594 | 15 | 594 | ACE [Arabidopsis thaliana] |
| RefSeq | NP_001154469.1 | 0 | 15 | 594 | 15 | 567 | HTH (HOTHEAD); FAD binding / aldehyde-lyase/ mandelonitrile lyase [Arabidopsis thaliana] |
| RefSeq | NP_565050.1 | 0 | 15 | 594 | 15 | 594 | HTH (HOTHEAD); FAD binding / aldehyde-lyase/ mandelonitrile lyase [Arabidopsis thaliana] |
| RefSeq | XP_002329018.1 | 0 | 32 | 594 | 29 | 591 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002527404.1 | 0 | 34 | 593 | 38 | 596 | glucose-methanol-choline (gmc) oxidoreductase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3gdp_B | 0 | 60 | 587 | 24 | 517 | A Chain A, Solution Structure Of The C-Terminal Domain Ole E 9 |
| PDB | 3gdp_A | 0 | 60 | 587 | 24 | 517 | A Chain A, Solution Structure Of The C-Terminal Domain Ole E 9 |
| PDB | 3gdn_B | 0 | 60 | 587 | 24 | 517 | A Chain A, Almond Hydroxynitrile Lyase In Complex With Benzaldehyde |
| PDB | 3gdn_A | 0 | 60 | 587 | 24 | 517 | A Chain A, Almond Hydroxynitrile Lyase In Complex With Benzaldehyde |
| PDB | 1ju2_B | 0 | 60 | 587 | 24 | 517 | A Chain A, Crystal Structure Of The Hydroxynitrile Lyase From Almond |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cutin biosynthesis | RXN-2121 | EC-1.1.1 | 18-hydroxyoleate dehydrogenase |
| cutin biosynthesis | RXN-9802 | EC-1.1.1 | 16-hydroxypalmitate dehydrogenase |
| suberin biosynthesis | RXN-2121 | EC-1.1.1 | 18-hydroxyoleate dehydrogenase |
