| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra004054 |
| Family | GT20 |
| Protein Properties | Length: 864 Molecular Weight: 98040.5 Isoelectric Point: 6.4019 |
| Chromosome | Chromosome/Scaffold: 07 Start: 16559007 End: 16561771 |
| Description | UDP-Glycosyltransferase / trehalose-phosphatase family protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 50 | 559 | 0 |
| SSDLSPKDRIIIVANELPIRAQRKLETSSTSTSTTHCSSKGWTFSLDENSLLLQLKDGLGSEATTTEVIYVGCLKEDIHPNEQEEVYQLLLENFKCVPTF LPLDLYTRYYHGFCKQQLWPLFHYMLPLSPDLGGRFDRSLWQAYVSVNKIFADRIMEVINPEDDFVWIHDYHLMVLPTFLRKRFNRVKLGFFLHSPFPSS EIYKTLPIREELLRALLNADLIGFHTFDYARHFLSCCSRMLGLTYESKRGYIGLEYYGRTVSIKILPVGIHMGQLRSVLSLPETENKVKELIEEYSKKGR RMLLGVDDMDIFKGITLKLLAMEQMLTQHPEWQGKVVLVQIANPARGKGKDVKEMKAETYSTVKRINQAFGRPGYDPIVLIDAPLRFYERVAYYVVAECC LVTAVRDGMNLIPYEYIVSRQGNEKLDKILKVENHHKSMLVVSEFIGCSPSLSGAIRVNPWNVDAVADAMDSALEVAEPEKQLRHEKHYKYVSTHDVGYW ARSFLQDLER | |||
| Full Sequence |
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| Protein Sequence Length: 864 Download |
| MVSRSYSNLL ELASGDSPTF GRMNRQIPRI MAVAGIMSNI DNDSKESSPS SDLSPKDRII 60 IVANELPIRA QRKLETSSTS TSTTHCSSKG WTFSLDENSL LLQLKDGLGS EATTTEVIYV 120 GCLKEDIHPN EQEEVYQLLL ENFKCVPTFL PLDLYTRYYH GFCKQQLWPL FHYMLPLSPD 180 LGGRFDRSLW QAYVSVNKIF ADRIMEVINP EDDFVWIHDY HLMVLPTFLR KRFNRVKLGF 240 FLHSPFPSSE IYKTLPIREE LLRALLNADL IGFHTFDYAR HFLSCCSRML GLTYESKRGY 300 IGLEYYGRTV SIKILPVGIH MGQLRSVLSL PETENKVKEL IEEYSKKGRR MLLGVDDMDI 360 FKGITLKLLA MEQMLTQHPE WQGKVVLVQI ANPARGKGKD VKEMKAETYS TVKRINQAFG 420 RPGYDPIVLI DAPLRFYERV AYYVVAECCL VTAVRDGMNL IPYEYIVSRQ GNEKLDKILK 480 VENHHKSMLV VSEFIGCSPS LSGAIRVNPW NVDAVADAMD SALEVAEPEK QLRHEKHYKY 540 VSTHDVGYWA RSFLQDLERS CGEHGRRRCW GIGFGLSFRV VALDQSFRKL SMEHIVSAYK 600 RTKTRAILLD YDDTLMPQGS IDKRPSSKSI EILNTLCRDK SNLVFIVSAK SRETLSDWFS 660 PCEKLGIAAE HGYFLRLRKD VEWENCVAAA DCSWKQIAEP VMELYTETTD GSTIEDKETA 720 LVWSYEDADP DFGSCQAKEL LDHLESVLAN EPVTVKRGQN YVEVKPQGVS KGLIARRMLS 780 MMQEKGTPPE FVLCIGDDRS DEDMFEVICS STEGPSIAPR AEVFACTVGQ KPSKAKYYLD 840 DTTEIVRLMH GLASVSEQTM SAV* 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03063 | PLN03063 | 1.0e-168 | 58 | 861 | 841 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN02205 | PLN02205 | 0 | 1 | 861 | 866 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming] | ||
| pfam00982 | Glyco_transf_20 | 0 | 58 | 559 | 505 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| PRK14501 | PRK14501 | 0 | 58 | 855 | 805 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| cd03788 | GT1_TPS | 0 | 58 | 558 | 501 | + Trehalose-6-Phosphate Synthase (TPS) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAG52003.1 | 0 | 1 | 858 | 1 | 843 | AC012563_13 putative trehalose-6-phosphate synthase; 46897-44149 [Arabidopsis thaliana] |
| RefSeq | NP_974105.1 | 0 | 1 | 858 | 1 | 856 | ATTPS6; alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups / trehalose-phosphatase [Arabidopsis thaliana] |
| RefSeq | XP_002284972.1 | 0 | 1 | 858 | 1 | 850 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002312472.1 | 0 | 1 | 861 | 1 | 850 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002314777.1 | 0 | 1 | 861 | 1 | 839 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 0 | 158 | 559 | 76 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 0 | 158 | 559 | 76 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 0 | 158 | 559 | 76 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 0 | 158 | 559 | 76 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1gz5_D | 0 | 158 | 559 | 75 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO777775 | 774 | 91 | 861 | 0 |
| HO779327 | 519 | 112 | 628 | 0 |
| HO779327 | 236 | 622 | 857 | 0 |
| HO795560 | 430 | 435 | 861 | 0 |
| HO777775 | 78 | 1 | 75 | 0.000000000008 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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