y
Basic Information | |
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Species | Brassica rapa |
Cazyme ID | Bra004319 |
Family | GH31 |
Protein Properties | Length: 927 Molecular Weight: 103325 Isoelectric Point: 7.2989 |
Chromosome | Chromosome/Scaffold: 07 Start: 17956724 End: 17960476 |
Description | alpha-xylosidase 1 |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH31 | 268 | 759 | 0 |
HFVAGPSPLDVVDQYTSLIGRPAPMPYWSLGFHQCRWGYRNLSVIEDVVDSYQKAKIPLDVIWNDDDHMDAKKDFTLSPISYPRAKLLNFLDRIHKMGMK YVVINDPGIGVNSSYGVYQRGMANDVFIKYEGKPFLAQVWPGAVHFPDFLNPKTVSWWGEEIRRFHELAPIDGLWIDMNEVSNFCSGLCTIPKGKKCPTG EGPGWICCLDCKNITKTRWDDPPYKINATGVTAPIGFKTIATSALHYNGVREYDAHSIYGFSETVATHKGLLSIEGKRPFILSRSTFVGSGKYAAHWTGD NQGTWQSLQVSISTMLNFGIFGVPMVGSDICGFYPQPTEELCNRWIEVGAFYPFSRDHANYYSPRQELYQWDTVAESARNALGMRYKILPYLYTLNYEAH MTGAPIARPLFFSFPEYTECYGSSRQFLLGSGLMISPVLEQGKTEVEALFPPGSWYHMFDMTQAVVSKKGKRVTLPAPLNFVNVHLYQNTIL |
Full Sequence |
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Protein Sequence Length: 927 Download |
MVTSRIVFSS LSLLVALILC FSPIHSSNTI GKGYRLISIE ESPDGGFIGY LQVKQKNKIY 60 GSDITTLRLY VKHETDTRLR VHITDAKKQR WEVPYNLLPR EQPPPVGKVI GKSRKSPITV 120 QELSGSELIF SYTTDPFSFA VKRKSNGQTI FNTSSNSAFG EMVFKDQYLE ISTSLPKDAS 180 IYGLGENSQA NGIKLVPNEP YTLYTEDVSA INLNTDLYGS HPVYMDLRNV GGKPYAHAVL 240 LLNSNGMDVF YRGTSLTYKV IGGVFDFHFV AGPSPLDVVD QYTSLIGRPA PMPYWSLGFH 300 QCRWGYRNLS VIEDVVDSYQ KAKIPLDVIW NDDDHMDAKK DFTLSPISYP RAKLLNFLDR 360 IHKMGMKYVV INDPGIGVNS SYGVYQRGMA NDVFIKYEGK PFLAQVWPGA VHFPDFLNPK 420 TVSWWGEEIR RFHELAPIDG LWIDMNEVSN FCSGLCTIPK GKKCPTGEGP GWICCLDCKN 480 ITKTRWDDPP YKINATGVTA PIGFKTIATS ALHYNGVREY DAHSIYGFSE TVATHKGLLS 540 IEGKRPFILS RSTFVGSGKY AAHWTGDNQG TWQSLQVSIS TMLNFGIFGV PMVGSDICGF 600 YPQPTEELCN RWIEVGAFYP FSRDHANYYS PRQELYQWDT VAESARNALG MRYKILPYLY 660 TLNYEAHMTG APIARPLFFS FPEYTECYGS SRQFLLGSGL MISPVLEQGK TEVEALFPPG 720 SWYHMFDMTQ AVVSKKGKRV TLPAPLNFVN VHLYQNTILP MQQGGLISKE ARTTPFSLVI 780 TFPAGASEGY AKGKLYLDED ELPEMKLGNG QSTYVDFYAS VGNGTVKMWS EVKEGKFALS 840 KGWVIEMVSV LGLRGGGGQA SEIVINGSPV SNEGKKVEVN LKEHTYVVGL EEEGENKSVM 900 VEVRGLEMLV GKDFNMSWKM SLNGAN* 960 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
pfam01055 | Glyco_hydro_31 | 8.0e-83 | 268 | 454 | 187 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
cd06602 | GH31_MGAM_SI_GAA | 2.0e-90 | 520 | 688 | 170 | + This family includes the following three closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), and lysosomal acid alpha-glucosidase (GAA), also known as acid-maltase. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end, and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). | ||
cd06603 | GH31_GANC_GANAB_alpha | 1.0e-98 | 287 | 670 | 391 | + This family includes the closely related glycosyl hydrolase family 31 (GH31) isozymes, neutral alpha-glucosidase C (GANC) and the alpha subunit of heterodimeric neutral alpha-glucosidase AB (GANAB). Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. The GANC/GANAB family includes the alpha-glucosidase II (ModA) from Dictyostelium discoideum as well as the alpha-glucosidase II (GLS2, or ROT2 - Reversal of TOR2 lethality protein 2) from Saccharomyces cerevisiae. | ||
pfam01055 | Glyco_hydro_31 | 1.0e-107 | 512 | 759 | 252 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
cd06604 | GH31_glucosidase_II_MalA | 1.0e-117 | 287 | 670 | 390 | + Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAD05539.1 | 0 | 20 | 923 | 13 | 907 | alpha-xylosidase precursor [Arabidopsis thaliana] |
RefSeq | NP_177023.1 | 0 | 20 | 923 | 21 | 915 | XYL1 (ALPHA-XYLOSIDASE 1); alpha-N-arabinofuranosidase/ hydrolase, hydrolyzing O-glycosyl compounds / xylan 1,4-beta-xylosidase [Arabidopsis thaliana] |
RefSeq | XP_002311455.1 | 0 | 22 | 924 | 4 | 910 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002315944.1 | 0 | 20 | 924 | 19 | 928 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002531635.1 | 0 | 12 | 924 | 10 | 930 | alpha-glucosidase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3w38_A | 0 | 5 | 925 | 8 | 911 | A Chain A, Structure Of The Thermostable Pectate Lyase Pl 47 |
PDB | 3w37_A | 0 | 5 | 925 | 8 | 911 | A Chain A, Structure Of The Thermostable Pectate Lyase Pl 47 |
PDB | 3lpp_D | 0 | 59 | 918 | 105 | 898 | A Chain A, Structure Of The Thermostable Pectate Lyase Pl 47 |
PDB | 3lpp_C | 0 | 59 | 918 | 105 | 898 | A Chain A, Structure Of The Thermostable Pectate Lyase Pl 47 |
PDB | 3lpp_B | 0 | 59 | 918 | 105 | 898 | A Chain A, Structure Of The Thermostable Pectate Lyase Pl 47 |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
starch degradation I | RXN-2141 | EC-3.2.1.20 | α-glucosidase |