| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra004326 |
| Family | CE10 |
| Protein Properties | Length: 335 Molecular Weight: 37006.4 Isoelectric Point: 6.635 |
| Chromosome | Chromosome/Scaffold: 07 Start: 18000235 End: 18001239 |
| Description | alpha/beta-Hydrolases superfamily protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CE10 | 70 | 312 | 0 |
| TNVWGRLYVPTVTNSTSVSKLPLIVYFHGGGFCAGSAYWSCYHQFLGRLSAKSRCLVMHVNYRLAPENPLPAAYEDGVNAILWLKKTRNDNLWSKQCDFG SIFLAGDSAGGNIANHVAGRLAAADALIKPLKIEGTILIQPFFGGEARTETERRVENNTESSVLTLASSDTWWRLTLPREANREHPYCKPVKITTRTLVC VAEMDVLMDRAMEMCDSDKKMIRCVVYKGVGHAFQVLGESQLA | |||
| Full Sequence |
|---|
| Protein Sequence Length: 335 Download |
| MEATTLITHV TTNNTNNTSI HGPVVEEVEG LIRVYKDGHV ERSQFVPCVD PSLPLELGVA 60 CSDVHIDKLT NVWGRLYVPT VTNSTSVSKL PLIVYFHGGG FCAGSAYWSC YHQFLGRLSA 120 KSRCLVMHVN YRLAPENPLP AAYEDGVNAI LWLKKTRNDN LWSKQCDFGS IFLAGDSAGG 180 NIANHVAGRL AAADALIKPL KIEGTILIQP FFGGEARTET ERRVENNTES SVLTLASSDT 240 WWRLTLPREA NREHPYCKPV KITTRTLVCV AEMDVLMDRA MEMCDSDKKM IRCVVYKGVG 300 HAFQVLGESQ LANTLTLQML CDIDAFIHHS EPLN* 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00135 | COesterase | 2.0e-7 | 71 | 180 | 123 | + Carboxylesterase family. | ||
| cd00312 | Esterase_lipase | 2.0e-7 | 76 | 186 | 127 | + Esterases and lipases (includes fungal lipases, cholinesterases, etc.) These enzymes act on carboxylic esters (EC: 3.1.1.-). The catalytic apparatus involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine.These catalytic residues are responsible for the nucleophilic attack on the carbonyl carbon atom of the ester bond. In contrast with other alpha/beta hydrolase fold family members, p-nitrobenzyl esterase and acetylcholine esterase have a Glu instead of Asp at the active site carboxylate. | ||
| PRK10162 | PRK10162 | 2.0e-14 | 75 | 303 | 238 | + acetyl esterase; Provisional | ||
| COG0657 | Aes | 5.0e-29 | 42 | 307 | 275 | + Esterase/lipase [Lipid metabolism] | ||
| pfam07859 | Abhydrolase_3 | 2.0e-57 | 93 | 304 | 220 | + alpha/beta hydrolase fold. This catalytic domain is found in a very wide range of enzymes. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008152 | metabolic process |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABB89015.1 | 0 | 1 | 318 | 1 | 329 | CXE carboxylesterase [Actinidia deliciosa] |
| RefSeq | NP_564936.1 | 0 | 5 | 334 | 4 | 336 | hydrolase [Arabidopsis thaliana] |
| RefSeq | XP_002278939.1 | 0 | 1 | 327 | 6 | 345 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002311450.1 | 0 | 21 | 327 | 23 | 346 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002529140.1 | 0 | 21 | 327 | 22 | 350 | catalytic, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2o7v_A | 0 | 59 | 305 | 53 | 310 | A Chain A, The Structure Of Endoglucanase From Termite, Nasutitermes Takasagoensis, At Ph 2.5. |
| PDB | 2o7r_A | 0 | 59 | 305 | 53 | 310 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 2zsi_A | 7e-38 | 58 | 289 | 69 | 311 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 2zsh_A | 7e-38 | 58 | 289 | 69 | 311 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| PDB | 3ed1_F | 4e-36 | 43 | 293 | 63 | 320 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| formononetin biosynthesis | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| formononetin biosynthesis | RXN-3625 | - | 2,7-dihydroxy-4'-methoxyisoflavanone dehydratase |
| isoflavonoid biosynthesis I | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-3303 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-5502 | - | 2,7,5-trihydroxy-4'-methoxyisoflavanone dehydratase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| DK480522 | 282 | 47 | 328 | 0 |
| EX095072 | 214 | 1 | 214 | 0 |
| FY439288 | 260 | 19 | 277 | 0 |
| DK540120 | 214 | 1 | 214 | 0 |
| EX097462 | 198 | 1 | 198 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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