| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra004590 |
| Family | GH77 |
| Protein Properties | Length: 942 Molecular Weight: 107873 Isoelectric Point: 5.7762 |
| Chromosome | Chromosome/Scaffold: 05 Start: 925034 End: 929735 |
| Description | disproportionating enzyme 2 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH77 | 276 | 924 | 0 |
| VPMFSVRSEDDVGVGEFLDLKLLVDWSVDSGLHLVQLLPVNDTSVHKMWWDSYPYSSLSVFALHPLYLRVQALSESLPADIKEQIEKAKKQLDKNDVDYE ATMETKLSIAKKIFDLEKDQTLSSSSFQKYFSENEGWLKPYAAFCFLRDFFETSDHSQWGTFSDYTEDKLEKLTSKDSLHYNTICFHYYIQYHLHVQLSE ASEYARKRGVVLKGDLPIGVDRNSVDTWVNKNLFRMNTSTGAPPDYFDKNGQNWGFPTYNWEEMSKDNYGWWRARLTQMGNYFTAYRIDHILGFFRIWEL PAHSMTGLVGKFRPSIPLSQEELEKEGIWDFDRLSKPYVQKKFLEEKFGDFWPFIASNFLNETQKDMYEFKEDCNTEKKIAAKLKSLAAKSLLLENEDKV RRDVFDILRNVVLIKDPEDARKFYPRFNIEDTSSFQDLDDHSKNVLKRLYYDYYFHRQEDLWRKNALKTLPALLNSSNMLACGEDLGLVPACVHPVMKEL ALVGLRIQRMPSESDVEFGVPSNYDYMTVCAPSCHDCSTLRAWWEEDEERRQRYFKEVIGLDAIPPSQCVPEITHFILRQHVEAPSMWAIFPLQDMLALK EEYTARPAKEETINDPTNPKHYWRYRVHVTLDSLMKDVDLKSTIKNLVS | |||
| Full Sequence |
|---|
| Protein Sequence Length: 942 Download |
| MMNLGTLSLT TTVKSSKPMV SLSFWIPYFT HWGQSLLVCG SAPGLGSGNV KKGLLLKPSQ 60 QEDQLVWSGS VSVPPGFTCD YCYYVVDDLK NVLRTEFGMK RKLVVPEALT GGESVQLRDL 120 WQSGDQALPF RSAFKDVIFR NTGDVEVGKP LGVFENKSDQ DDSVVVQFKI CCPDVGEGTS 180 VYVLGTSGKL GKWKVESGLK LSYVGDSMWE GDCLIPKAEF PIKYRYCKVP KDGSIGLESG 240 GNRELTLHSS SNKQEYIVMS DGLFREMPWR GAGVAVPMFS VRSEDDVGVG EFLDLKLLVD 300 WSVDSGLHLV QLLPVNDTSV HKMWWDSYPY SSLSVFALHP LYLRVQALSE SLPADIKEQI 360 EKAKKQLDKN DVDYEATMET KLSIAKKIFD LEKDQTLSSS SFQKYFSENE GWLKPYAAFC 420 FLRDFFETSD HSQWGTFSDY TEDKLEKLTS KDSLHYNTIC FHYYIQYHLH VQLSEASEYA 480 RKRGVVLKGD LPIGVDRNSV DTWVNKNLFR MNTSTGAPPD YFDKNGQNWG FPTYNWEEMS 540 KDNYGWWRAR LTQMGNYFTA YRIDHILGFF RIWELPAHSM TGLVGKFRPS IPLSQEELEK 600 EGIWDFDRLS KPYVQKKFLE EKFGDFWPFI ASNFLNETQK DMYEFKEDCN TEKKIAAKLK 660 SLAAKSLLLE NEDKVRRDVF DILRNVVLIK DPEDARKFYP RFNIEDTSSF QDLDDHSKNV 720 LKRLYYDYYF HRQEDLWRKN ALKTLPALLN SSNMLACGED LGLVPACVHP VMKELALVGL 780 RIQRMPSESD VEFGVPSNYD YMTVCAPSCH DCSTLRAWWE EDEERRQRYF KEVIGLDAIP 840 PSQCVPEITH FILRQHVEAP SMWAIFPLQD MLALKEEYTA RPAKEETIND PTNPKHYWRY 900 RVHVTLDSLM KDVDLKSTIK NLVSSSGRSV PASGEDVNNK K* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam02446 | Glyco_hydro_77 | 1.0e-49 | 748 | 926 | 180 | + 4-alpha-glucanotransferase. These enzymes EC:2.4.1.25 transfer a segment of a (1,4)-alpha-D-glucan to a new 4-position in an acceptor, which may be glucose or (1,4)-alpha-D-glucan. | ||
| COG1640 | MalQ | 3.0e-70 | 265 | 577 | 314 | + 4-alpha-glucanotransferase [Carbohydrate transport and metabolism] | ||
| pfam02446 | Glyco_hydro_77 | 8.0e-126 | 276 | 611 | 338 | + 4-alpha-glucanotransferase. These enzymes EC:2.4.1.25 transfer a segment of a (1,4)-alpha-D-glucan to a new 4-position in an acceptor, which may be glucose or (1,4)-alpha-D-glucan. | ||
| PLN02950 | PLN02950 | 0 | 18 | 919 | 904 | + 4-alpha-glucanotransferase | ||
| PLN03236 | PLN03236 | 0 | 257 | 929 | 700 | + 4-alpha-glucanotransferase; Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004134 | 4-alpha-glucanotransferase activity |
| GO:0005975 | carbohydrate metabolic process |
| GO:2001070 | starch binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAR99599.1 | 0 | 22 | 931 | 11 | 919 | 4-alpha-glucanotransferase [Solanum tuberosum] |
| EMBL | CBI32836.1 | 0 | 1 | 935 | 1 | 932 | unnamed protein product [Vitis vinifera] |
| RefSeq | NP_181616.3 | 0 | 1 | 941 | 1 | 942 | DPE2 (DISPROPORTIONATING ENZYME 2); 4-alpha-glucanotransferase/ heteroglycan binding [Arabidopsis thaliana] |
| RefSeq | XP_002308854.1 | 0 | 1 | 935 | 1 | 946 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002323208.1 | 0 | 1 | 935 | 6 | 896 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2x1i_A | 2e-26 | 304 | 587 | 38 | 316 | A Chain A, Glycoside Hydrolase Family 77 4-Alpha-Glucanotransferase From Thermus Brockianus |
| PDB | 1tz7_B | 4e-26 | 270 | 587 | 22 | 329 | A Chain A, Aquifex Aeolicus Amylomaltase |
| PDB | 1tz7_B | 0.00004 | 757 | 930 | 351 | 505 | A Chain A, Aquifex Aeolicus Amylomaltase |
| PDB | 1tz7_A | 4e-26 | 270 | 587 | 22 | 329 | A Chain A, Aquifex Aeolicus Amylomaltase |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-1828 | EC-2.4.1.25 | 4-α-glucanotransferase |
| starch degradation II | RXN-12391 | EC-2.4.1.25 | 4-α-glucanotransferase |
| Hydropathy |
|---|
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO794605 | 533 | 406 | 938 | 0 |
| HO794605 | 305 | 117 | 421 | 0 |
| HO779714 | 519 | 126 | 642 | 0 |
| HO476301 | 430 | 472 | 897 | 0 |
| EG449989 | 284 | 600 | 883 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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