| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra004924 |
| Family | CE10 |
| Protein Properties | Length: 325 Molecular Weight: 36117.9 Isoelectric Point: 6.3478 |
| Chromosome | Chromosome/Scaffold: 05 Start: 2518220 End: 2519194 |
| Description | alpha/beta-Hydrolases superfamily protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CE10 | 32 | 301 | 0 |
| KLPPTEHSKDIPLNPNNKTFIRLFKPRNIPPPETKLPILIYFHGGGFILYSAASAPFHESCTKMADRLQTMILSVEYRLSPEHRLPAAYDDGVDAISWLR DQARNGGDCDTWLSDVDFSRCFVMGSSSGGNIVYSVALRVAETDLSPVKIRGLIMNQAFFGGVEPSDSESRLKDDRICPLTATHLLWSLCLPHGVDRDHV YSNPIKSSGAEEREKMGRFPSTLINGYGGDPLVDRQRDVAEMLKARGVHVETRFDKDGFHACELFDENKA | |||
| Full Sequence |
|---|
| Protein Sequence Length: 325 Download |
| MEAPPPSSDP YKFLNITLNS DGSLTRHRDF PKLPPTEHSK DIPLNPNNKT FIRLFKPRNI 60 PPPETKLPIL IYFHGGGFIL YSAASAPFHE SCTKMADRLQ TMILSVEYRL SPEHRLPAAY 120 DDGVDAISWL RDQARNGGDC DTWLSDVDFS RCFVMGSSSG GNIVYSVALR VAETDLSPVK 180 IRGLIMNQAF FGGVEPSDSE SRLKDDRICP LTATHLLWSL CLPHGVDRDH VYSNPIKSSG 240 AEEREKMGRF PSTLINGYGG DPLVDRQRDV AEMLKARGVH VETRFDKDGF HACELFDENK 300 AKALYDTVEG FMKSCSMTAP SSNM* 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00312 | Esterase_lipase | 0.0007 | 54 | 184 | 142 | + Esterases and lipases (includes fungal lipases, cholinesterases, etc.) These enzymes act on carboxylic esters (EC: 3.1.1.-). The catalytic apparatus involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine.These catalytic residues are responsible for the nucleophilic attack on the carbonyl carbon atom of the ester bond. In contrast with other alpha/beta hydrolase fold family members, p-nitrobenzyl esterase and acetylcholine esterase have a Glu instead of Asp at the active site carboxylate. | ||
| COG2272 | PnbA | 1.0e-5 | 63 | 164 | 118 | + Carboxylesterase type B [Lipid metabolism] | ||
| PRK10162 | PRK10162 | 1.0e-10 | 53 | 292 | 244 | + acetyl esterase; Provisional | ||
| COG0657 | Aes | 4.0e-32 | 30 | 297 | 277 | + Esterase/lipase [Lipid metabolism] | ||
| pfam07859 | Abhydrolase_3 | 1.0e-63 | 70 | 292 | 223 | + alpha/beta hydrolase fold. This catalytic domain is found in a very wide range of enzymes. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008152 | metabolic process |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAM65132.1 | 0 | 1 | 323 | 1 | 326 | unknown [Arabidopsis thaliana] |
| EMBL | CAN60859.1 | 0 | 3 | 324 | 5 | 336 | hypothetical protein [Vitis vinifera] |
| RefSeq | NP_566047.1 | 0 | 1 | 323 | 1 | 326 | hydrolase [Arabidopsis thaliana] |
| RefSeq | XP_002320078.1 | 0 | 9 | 322 | 2 | 324 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002511752.1 | 0 | 9 | 313 | 18 | 335 | Gibberellin receptor GID1, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2o7v_A | 0 | 6 | 316 | 14 | 332 | B Chain B, Crystal Structure Of The Complex Between Pectin Methylesterase And Its Inhibitor Protein |
| PDB | 2o7r_A | 0 | 6 | 316 | 14 | 332 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 3ed1_F | 4e-36 | 62 | 283 | 107 | 317 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 3ed1_E | 4e-36 | 62 | 283 | 107 | 317 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 3ed1_D | 4e-36 | 62 | 283 | 107 | 317 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| formononetin biosynthesis | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| formononetin biosynthesis | RXN-3625 | - | 2,7-dihydroxy-4'-methoxyisoflavanone dehydratase |
| isoflavonoid biosynthesis I | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-3303 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-5502 | - | 2,7,5-trihydroxy-4'-methoxyisoflavanone dehydratase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EV566807 | 229 | 1 | 228 | 0 |
| DK520487 | 219 | 110 | 325 | 0 |
| CD843898 | 201 | 15 | 213 | 0 |
| EL587730 | 200 | 5 | 202 | 0 |
| EL628854 | 183 | 68 | 250 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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