| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra004925 |
| Family | CE10 |
| Protein Properties | Length: 325 Molecular Weight: 36032 Isoelectric Point: 6.1667 |
| Chromosome | Chromosome/Scaffold: 05 Start: 2520015 End: 2520989 |
| Description | alpha/beta-Hydrolases superfamily protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CE10 | 50 | 314 | 0 |
| DITINLETGVTVRIFRPTNLPSNDNTIARLPILIHLHGSGWVLYPANSPANSRGCSQMASELTVIIVSVNYRLAPENRLPTQYDDALEALLWVKRQAVDQ TNGEPWLRDYADFSRCYIFGSSTGANLAFQLALRSLDHDLTPLKIDGSVFYQPFYGGKARTKAELKNFADPVMPVPAIDAMWELSLPKGVDRDHRYCNPV GYLPQKEKVGRLGRCLVIGYGGDTLVDHQQDFVEMLVTAGVRVEAKFDDAGFHGIELVDPRRAVA | |||
| Full Sequence |
|---|
| Protein Sequence Length: 325 Download |
| MSRDPVPAFD AYKHLNLSIN PDGSCTRNFE YPIVDPDPSP SPGKLTASKD ITINLETGVT 60 VRIFRPTNLP SNDNTIARLP ILIHLHGSGW VLYPANSPAN SRGCSQMASE LTVIIVSVNY 120 RLAPENRLPT QYDDALEALL WVKRQAVDQT NGEPWLRDYA DFSRCYIFGS STGANLAFQL 180 ALRSLDHDLT PLKIDGSVFY QPFYGGKART KAELKNFADP VMPVPAIDAM WELSLPKGVD 240 RDHRYCNPVG YLPQKEKVGR LGRCLVIGYG GDTLVDHQQD FVEMLVTAGV RVEAKFDDAG 300 FHGIELVDPR RAVALLNMIR EFIN* 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00135 | COesterase | 0.001 | 63 | 174 | 123 | + Carboxylesterase family. | ||
| cd00312 | Esterase_lipase | 6.0e-7 | 60 | 174 | 129 | + Esterases and lipases (includes fungal lipases, cholinesterases, etc.) These enzymes act on carboxylic esters (EC: 3.1.1.-). The catalytic apparatus involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine.These catalytic residues are responsible for the nucleophilic attack on the carbonyl carbon atom of the ester bond. In contrast with other alpha/beta hydrolase fold family members, p-nitrobenzyl esterase and acetylcholine esterase have a Glu instead of Asp at the active site carboxylate. | ||
| PRK10162 | PRK10162 | 3.0e-7 | 47 | 205 | 161 | + acetyl esterase; Provisional | ||
| COG0657 | Aes | 6.0e-32 | 31 | 324 | 297 | + Esterase/lipase [Lipid metabolism] | ||
| pfam07859 | Abhydrolase_3 | 5.0e-52 | 82 | 303 | 222 | + alpha/beta hydrolase fold. This catalytic domain is found in a very wide range of enzymes. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008152 | metabolic process |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAN60859.1 | 0 | 2 | 323 | 4 | 327 | hypothetical protein [Vitis vinifera] |
| RefSeq | NP_182085.1 | 0 | 1 | 324 | 1 | 324 | hydrolase [Arabidopsis thaliana] |
| RefSeq | XP_002301311.1 | 0 | 6 | 324 | 1 | 325 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002511753.1 | 0 | 9 | 324 | 4 | 315 | Gibberellin receptor GID1, putative [Ricinus communis] |
| RefSeq | XP_002511754.1 | 0 | 9 | 323 | 4 | 317 | Gibberellin receptor GID1, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2o7v_A | 0 | 7 | 323 | 14 | 327 | A Chain A, Cocrystal Structure Of The Flagellar SigmaANTI-Sigma Complex, Sigma-28FLGM |
| PDB | 2o7r_A | 0 | 7 | 323 | 14 | 327 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 3ed1_F | 5e-28 | 16 | 293 | 31 | 316 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 3ed1_E | 5e-28 | 16 | 293 | 31 | 316 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 3ed1_D | 5e-28 | 16 | 293 | 31 | 316 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| formononetin biosynthesis | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| formononetin biosynthesis | RXN-3625 | - | 2,7-dihydroxy-4'-methoxyisoflavanone dehydratase |
| isoflavonoid biosynthesis I | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-3303 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-5502 | - | 2,7,5-trihydroxy-4'-methoxyisoflavanone dehydratase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| GR437662 | 193 | 1 | 193 | 0 |
| EH771231 | 271 | 9 | 277 | 0 |
| EB424893 | 266 | 9 | 272 | 0 |
| GR068161 | 235 | 78 | 312 | 0 |
| DY288919 | 320 | 8 | 324 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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