| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra005269 |
| Family | GH13 |
| Protein Properties | Length: 846 Molecular Weight: 96022 Isoelectric Point: 4.8752 |
| Chromosome | Chromosome/Scaffold: 05 Start: 4424873 End: 4430184 |
| Description | starch branching enzyme 2.1 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH13 | 343 | 664 | 2e-29 |
| LPRIKKLGYNAVQIMAIQEHSYYASFGYHVTNFFAPSSRFGTPDDLKSLIDKAHELGLVVLMDIVHSHASKNTLDGLNMFDGTDGQYFHSGERGYHWMWD SRLFNYGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLQVEFTGNYNEYFGYSTDVDAVVYLMLVNDMIHGLYPEAIVVGEDVSGMPAFCIP VEDGGVGFDYRLHMAVADKWIELLKKRDEDWQVGDIVFTLTNRRWGEKCVVYAESHDQALVGDKTIAFWLMDKDMYDFMAVDKQATPRVDRGIALHKMIR LITMGLGGEGYLNFMGNEFGHP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 846 Download |
| MVYTISGVRF PHLASIKNSS LPSFNDDRRT NAVTFSLRKD TRSSSGKIFA RKPSYDSESS 60 SSIATTASEN LGSHQSDSSS AETQETLSEV TQVHDDVDAQ KAETERQEQT STLSTSGGQS 120 YNENIASMPD SLDQEVVQRK IPPPGDGKKI YDIDPMLKSY DGHLDYRYGQ YIKLREEIDK 180 NEGGLEAFSR GYEIFGFTRS ATGITYREWA PGAKAASLIG DFNNWNSKAD VMTRNEYGVW 240 EIFLPNDADG SPAIPHGSRV KIRMDTTSGI KDSIPAWIKY SVQAPGEIPY DGVYYDPAEE 300 DKYVFKHPRP RKPTSLRIYE SHVGMSSTEP MINTYANFRD DVLPRIKKLG YNAVQIMAIQ 360 EHSYYASFGY HVTNFFAPSS RFGTPDDLKS LIDKAHELGL VVLMDIVHSH ASKNTLDGLN 420 MFDGTDGQYF HSGERGYHWM WDSRLFNYGS WEVLRYLLSN ARWWLEEYKF DGFRFDGVTS 480 MMYTHHGLQV EFTGNYNEYF GYSTDVDAVV YLMLVNDMIH GLYPEAIVVG EDVSGMPAFC 540 IPVEDGGVGF DYRLHMAVAD KWIELLKKRD EDWQVGDIVF TLTNRRWGEK CVVYAESHDQ 600 ALVGDKTIAF WLMDKDMYDF MAVDKQATPR VDRGIALHKM IRLITMGLGG EGYLNFMGNE 660 FGHPEWIDFP RTDQHLPDGR VIPGNNGSYD KCRRRFDLGD AEYLRYHGLQ EFDRAMQHLE 720 EKYGFMTSEH QYISRKDEGD RVIVFEKGNL VFVFNFHWTN SYSDYRIGCS VPGKYKIVLD 780 SDDSSFGGFN RLDDSAEFFT SDGKYDDRPC SLMVYAPCRT AVVYAAVDGD EDSSLVPISL 840 VPDDV* 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN02960 | PLN02960 | 2.0e-8 | 167 | 244 | 84 | + alpha-amylase | ||
| PLN03244 | PLN03244 | 7.0e-136 | 252 | 824 | 581 | + alpha-amylase; Provisional | ||
| PLN02447 | PLN02447 | 0 | 140 | 845 | 711 | + 1,4-alpha-glucan-branching enzyme | ||
| cd11321 | AmyAc_bac_euk_BE | 0 | 299 | 714 | 417 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| PLN02960 | PLN02960 | 0 | 252 | 824 | 577 | + alpha-amylase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| GO:0043169 | cation binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAB03099.1 | 0 | 8 | 845 | 4 | 854 | starch branching enzyme class II [Arabidopsis thaliana] |
| GenBank | ABO31359.1 | 0 | 1 | 835 | 1 | 847 | starch branching enzyme II-2 [Malus x domestica] |
| GenBank | ACA35286.1 | 0 | 1 | 832 | 1 | 874 | starch branching enzyme I [Cucumis sativus] |
| EMBL | CAA56319.1 | 0 | 1 | 840 | 1 | 855 | starch branching enzyme I [Pisum sativum] |
| RefSeq | NP_181180.1 | 0 | 1 | 845 | 1 | 858 | SBE2.1 (starch branching enzyme 2.1); 1,4-alpha-glucan branching enzyme [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3amk_A | 0 | 146 | 832 | 9 | 698 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 3vu2_B | 0 | 146 | 832 | 9 | 698 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 3vu2_A | 0 | 146 | 832 | 9 | 698 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 3aml_A | 0 | 150 | 832 | 13 | 698 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 1m7x_D | 0 | 201 | 790 | 24 | 579 | A Chain A, The X-Ray Crystallographic Structure Of Branching Enzyme |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch biosynthesis | RXN-7710 | EC-2.4.1.18 | 1,4-α-glucan branching enzyme |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO794536 | 692 | 144 | 835 | 0 |
| HO777638 | 636 | 200 | 835 | 0 |
| HO458123 | 395 | 433 | 827 | 0 |
| HO458123 | 296 | 144 | 432 | 0 |
| HO777638 | 47 | 153 | 199 | 0.00000002 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
